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The preference of tryptophan for membrane interfaces


, : The preference of tryptophan for membrane interfaces. Biochemistry 37(42): 14713-8

One of the ubiquitous features of membrane proteins is the preference of tryptophan and tyrosine residues for membrane surfaces that presumably arises from enhanced stability due to distinct interfacial interactions. The physical basis for this preference is widely believed to arise from amphipathic interactions related to imino-group hydrogen bonding and/or dipole interactions. We have examined these and other possibilities for tryptophan's interfacial preference by using 1H magic angle spinning (MAS) chemical shift measurements, two-dimensional (2D) nuclear Overhauser effect spectroscopy (2D-NOESY) 1H MAS NMR, and solid state 2H NMR to study the interactions of four tryptophan analogues with phosphatidylcholine membranes. We find that the analogues reside in the vicinity of the glycerol group where they all cause similar modest changes in acyl chain organization and that hydrocarbon penetration was not increased by reduction of hydrogen bonding or electric dipole interaction ability. These observations rule out simple amphipathic or dipolar interactions as the physical basis for the interfacial preference. More likely, the preference is dominated by tryptophan's flat rigid shape that limits access to the hydrocarbon core and its pi electronic structure and associated quadrupolar moment (aromaticity) that favor residing in the electrostatically complex interface environment.

US$19.90

PMID: 9778346

DOI: 10.1021/bi980809c


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Ramos, O.; Boguszewski, César.Luiz.; Teixeira, S.; D.B.m, R.; Parolim, B.; Prolla, Jão.Carlos., 2009: Performance of computed tomographic colonography for the screening of colorectal polyp in acromegalic patients: a prospective study. Acromegalic patients have better chances to develop colorectal polyps and cancer and, considered a high-risk group, need to undergo frequent screening examinations. Moreover, in acromegalia, the increased bowel length and the intestinal loop compl...

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Raghow, R.; Grace, T.D., 1974: Studies on a nuclear polyhedrosis virus in Bombyx mori cells in vitro. 1. Multiplication kinetics and ultrastructural studies. Journal of Ultrastructure Research 47(3): 384-399

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Sysoev, I.V., 1967: A study of trichocephaliasis in swine. Veterinariia 44(2): 60-62

Oprea, C.; Oprea, D.D., 1968: Morphological observations on the seeds of some species of the Vitaceae. Examples are given from the genera Vitis, Parthenocissus and Ampelopsis of differences in seed structure and morphology which are characteristics of the genus and in some cases of the species. It is suggested that seed types may serve as useful cr...

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Ott, G., 1971: Nur fur Spezialisten. Aquar-u Terrar Z: 24: 75-77

Harrison, G.; Lapp, N.L., 1972: Electron microscopic findings in the lungs of miners. Annals of the New York Academy of Sciences 200: 73-93

Chatterjee, S.N.; Chaudhuri, K., 2003: Lipopolysaccharides of Vibrio cholerae. I. Physical and chemical characterization. Vibrio cholerae is the causative organism of the disease cholera. The lipopolysaccharide (LPS) of V. cholerae plays an important role in eliciting the antibacterial immune response of the host and in classifying the vibrios into some 200 or more s...