geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Two apical multidrug transporters, P-gp and MRP2, are differently altered in chronic renal failure


, : Two apical multidrug transporters, P-gp and MRP2, are differently altered in chronic renal failure. American Journal of Physiology. Renal Physiology 280(4): F636-F645

Tubular function is altered in chronic renal failure (CRF). Whether drug secretion by renal tubules is modified in CRF is questioned because of frequent accumulation of various toxins in CRF. This function mainly involves ATP-dependent drug transporters, particularly P-glycoprotein (P-gp) and multidrug resistance-associated protein (MRP) 2, both present in apical membrane of epithelial cells. The present study was aimed at determining the changes in P-gp and MRP2 expression induced by experimental CRF in kidney and liver. The relationship between MRP2 and glutathione metabolism changes was examined because MRP2 transports GSSG and glutathione conjugates. Rats underwent either 80% subtotal nephrectomy (Nx) or sham operation, and determinations were performed 3 and 6 wk later. CRF induced a 70-200% rise in protein and mRNA expression of MRP2 after 3 and 6 wk post-Nx in remnant kidney and after 6 wk in liver. However, P-gp expression was unchanged by CRF. Relative to whole kidney mass, total MRP2 levels decreased by only 27% in Nx rats whereas total P-gp levels were reduced by 60%. Renal GSSG and total glutathione levels were increased by 30% in Nx rats, but glutathione-S-transferase (GST) activity was normal; liver GSSG levels and GST activity were reduced in Nx rats. In conclusion, CRF resulted in specific overexpression of MRP2 in kidney and liver. This could be an adaptative response to some elevated circulating toxins. The later MRP2 induction and different glutathione changes in liver compared with kidney suggest different mechanisms for MRP2 induction and/or action in these two tissues. Reprinted by permission of the publisher.

US$29.90

PMID: 11249855


Other references

Beardmore, J.M.; Richards, R.C., 1983: Concentrations of epidermal growth factor in mouse milk throughout lactation. A radioimmunoassay was used to measure the concentration of epidermal growth factor (EGF) in mouse milk throughout lactation. The EGF content of mouse milk increased steadily from birth to a concentration of 427 micrograms/l at day 8 of lactation....

Hovell, R., 2015: Dr yes or Dr no?. Veterinary Record 176(7): 178-179

Tsvelikhovsky, D.; Gelman, D.; Molander, G.A.; Blum, J., 2004: Lanthanide-promoted ethylation of Schiff bases by triethylaluminum. [reaction: see text] Schiff bases of aromatic aldehydes and anilines that fail to react with triethylaluminum are smoothly alkylated at room temperature in the presence of lanthanide catalysts. The alkylation takes place selectively at the vinylic...

Accardo-Palumbo, A.; Triolo, G.; Carbone, M.C.; Ferrante, A.; Ciccia, F.; Giardina, E.; Triolo, G., 2000: Polymorphonuclear leukocyte myeloperoxidase levels in patients with Behçet's disease. Myeloperoxidase was measured in the plasma and in the supernatants of polymorphonuclear granulocyte cultures from patients with Behçet's disease. High levels were found in both plasma and supernatants from patients with active disease. The a...

Amaral, A. d., 1927: Studies of Nearctic Ophidia. 2. Crotalus pricei Van Denburgh 1896 a synonym of C. triseriatus (Wagler) 1830. Bulletin of the Antivenin Institute of America Glenolden, 12: 48-54

Rivas-Vilchis, Jé.F.; Hernández-Sánchez, F.; González-Camarena, Rón.; Suárez-Rodríguez, L.D.; Escorcia-Gaona, R.; Cervantes-Reyes, J.A.; Román-Ramos, Rén., 2007: Assessment of the vascular effects of PC6 (Neiguan) using the second derivative of the finger photoplethysmogram in healthy and hypertensive subjects. Indices obtained from the second derivative of the digital volume pulse waveform have been proposed to characterize vascular aging, arterial rigidity, and the effects of vasoactive drugs. The purpose of this study was to assess the effects of manu...

Tavoussi M.; Avramis V.I., 1986: Pharmacodynamics of fludarabine phosphate in p 388 leukemia bearing mice after toxic and non toxic regimens. Proceedings American Society of Clinical Oncology Annual Meeting 5: 45

Landman, J.; Davidson, V.; Brown, R.; Hayward, G.; Otten, L., 1994: Modelling of a continuous peanut roasting process. Developments in food science: 07-214

Tanaka, N.; Shimuzu, Y.; Arima, S.; Harada, J., 1994: Fractal analysis of the structures of plant root systems. Plan and cubic fractal analyses were carried out on the root systems of soyabeans, groundnuts, maize, Phaseolus vulgaris, Medicago sativa, Vigna angularis, and Vigna unguiculata.

Egor'kov N.A.; Maksimova N.M.; Potemkina E.E.; Platonova T.V., 1989: Humoral immune status in vaccinated children who have had diphtheria. Zhurnal Mikrobiologii Epidemiologii i Immunobiologii (1): 94-95