+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Unique targeting of cytosolic phospholipase A2 to plasma membranes mediated by the NADPH oxidase in phagocytes

, : Unique targeting of cytosolic phospholipase A2 to plasma membranes mediated by the NADPH oxidase in phagocytes. Journal of Cell Biology 162(4): 683-692

Cytosolic phospholipase A2 (cPLA2)-generated arachidonic acid (AA) has been shown to be an essential requirement for the activation of NADPH oxidase, in addition to its being the major enzyme involved in the formation of eicosanoid at the nuclear membranes. The mechanism by which cPLA2 regulates NADPH oxidase activity is not known, particularly since the NADPH oxidase complex is localized in the plasma membranes of stimulated cells. The present study is the first to demonstrate that upon stimulation cPLA2 is transiently recruited to the plasma membranes by a functional NADPH oxidase in neutrophils and in granulocyte-like PLB-985 cells. Coimmunoprecipitation experiments and double labeling immunofluorescence analysis demonstrated the unique colocalization of cPLA2 and the NADPH oxidase in plasma membranes of stimulated cells, in correlation with the kinetic burst of superoxide production. A specific affinity in vitro binding was detected between GST-p47phox or GST-p67phox and cPLA2 in lysates of stimulated cells. The association between these two enzymes provides the molecular basis for AA released by cPLA2 to activate the assembled NADPH oxidase. The ability of cPLA2 to regulate two different functions in the same cells (superoxide generation and eicosanoid production) is achieved by a novel dual subcellular localization of cPLA2 to different targets. Reprinted by permission of the publisher.


PMID: 12913107

DOI: 10.1083/jcb.200211056

Other references

Osborn, T.J.; Hulme, M., 2003: Evidence for trends in heavy rainfall events over the UK. Daily precipitation in the UK has changed over the period 1961-2000, becoming on average more intense in winter and less intense in summer. Recent increases in total winter precipitation are shown to be mainly due to an increase in the amount of p...

Alvarez, G.; Visitación Bartolomé, M.; Miana, Mía.; Jurado-López, R.; Martín, R.; Zuluaga, P.; Martinez-Martinez, E.; Nieto, M.Luisa.; Alvarez-Sala, L.A.; Millán, Jús.; Lahera, V.; Cachofeiro, V., 2012: The effects of adiponectin and leptin on human endothelial cell proliferation: a live-cell study. The effect of adiponectin and leptin on the proliferation of the human microvascular endothelial cell line (HMEC-1) was studied in the absence or presence of fetal bovine serum (FBS). The participation of extracellular signal-regulated kinase (ERK...

Dong ShangSheng; Luo YaoPing; W.J.nJie; T.Y.uYing; Tong QiQing, 2000: Effect of shading and organic fertilizer on the alcoholic aroma production in summer tea leaves. The amounts of beta -glucosidase and 17 free or bound alcoholic aroma compounds in summer tea leaves were determined. The amount of bound aroma compounds was markedly higher than that of free aroma compounds in summer tea. Both shading and basal a...

Heimansohn, H., 1966: A dental order peg board. Dental Survey 42(11): 49-50

Light, D.W., 2007: Is G8 putting profits before the world's poorest children?. Lancet 370(9584): 297-298

Takahashi M.; Deguchi Y.; Kaneto H., 1988: Blockade of the development of analgesic tolerance to morphine by concurrent treatment with opioid mediated but not non opioid mediated stress in mice. Studies have been carried out to determine how the analgesic effect of morphine and the development of tolerance to the effect would be influenced by concurrent exposure to stresses in mice. Application of footshock (FS) stress, which produces ana...

Røiri, P., 2003: Marked increase of the number of myocardial infarctions. Tidsskrift for den Norske Laegeforening 123(24): 3628; Author Reply 3628-3628; Author Reply 3628

Kennedy, C.; Sokoloff, L., 1957: An adaptation of the nitrous oxide method to the study of the cerebral circulation in children; normal values for cerebral blood flow and cerebral metabolic rate in childhood. The nitrous oxide method for measuring cerebral blood flow has been adapted for use in normal children without requirement for sedation. On comparing the modified procedure with the original in adults, all cerebral circulatory functions were compa...

Geyer, G.; Linss, W.; Stibenz, D., 1973: Ultrahistochemical demonstration of positively charged groups at the surface of human red blood cells. Acta Histochemica 46(2): 244-252

Hirt, R.P.; Alsmark, C.; Embley, T.Martin., 2015: Lateral gene transfers and the origins of the eukaryote proteome: a view from microbial parasites. Our knowledge of the extent and functional impact of lateral gene transfer (LGT) from prokaryotes to eukaryotes, outside of endosymbiosis, is still rather limited. Here we review the recent literature, focusing mainly on microbial parasites, indic...