geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Anion selectivity of apical membrane conductance of Calu 3 human airway epithelium


, : Anion selectivity of apical membrane conductance of Calu 3 human airway epithelium. Pfluegers Archiv European Journal of Physiology 437(6): 812-822, May

Anion selectivity of the cystic fibrosis conductance transmembrane conductance regulator (CFTR) and other channels and parallel pathways expressed endogenously in apical membranes of polarized Calu-3 epithelial monolayers was studied under control conditions and during cAMP stimulation. Basolateral membranes were eliminated using alpha-toxin. The cAMP-stimulated, gradient-driven currents had the sequence Br>/=Cl>/=NO3>SCN> I>/=F>formate>HCO3>acetate>propionate=butyrate=ATP= PPi=PO4=SO4=0. Selectivity of parallel cAMP-independent pathway(s) was Br>Cl=SCN=NO3>I>formate=F >HCO3>acetate>propionate. SCN, I, F or formate blocked cAMP-stimulated, but not control, Cl currents. Anions >0.53 nm in diameter were impermeant, suggesting that the apical CFTR channel has a limiting diameter of 0.53 nm. The selectivity, blocking patterns and pore size of the cAMP-stimulated conductance pathway were very similar to those in previous reports in which CFTR was heterologously expressed in non-epithelial cells. Thus, CFTR appears to be the major apical anion conductance pathway in Calu-3 cells, and its conduction properties are independent of the expression system. CFTR in Calu-3 cells also conducts physiologically relevant anions, but not ATP, PO4 or SO4. A pathway parallel (probably a tight junction) showed a different selectivity than CFTR.

US$19.90

PMID: 10370058

DOI: 10.1007/s004240050850


Other references

Anonymous, 2012: Abstracts of the 11th Congress of the French Society of Vascular Medicine, 2012, Lyon, France. Journal des Maladies Vasculaires 37(5): 235-280

Yan, H.; Wang, C.; Marx, S.O.; Pitt, G.S., 2017: Calmodulin limits pathogenic Na+ channel persistent current. Increased "persistent" current, caused by delayed inactivation, through voltage-gated Na<sup>+</sup> (Na<sub>V</sub>) channels leads to cardiac arrhythmias or epilepsy. The underlying molecular contributors to the...

Yin, L.; Gater, S.T.; Karrer, K.M., 2010: A developmentally regulated gene, ASI2, is required for endocycling in the macronuclear anlagen of Tetrahymena. Ciliated protozoa contain two types of nuclei, germ line micronuclei (Mic) and transcriptionally active macronuclei (Mac). During sexual reproduction, the parental Mac degenerates and a new Mac develops from a mitotic product of the zygotic Mic. M...

Juarez, A.; Härtlein, M.; Goebel, W., 1984: Study of regulation and transport of hemolysin by using fusion of the beta-galactosidase gene (lacZ) to hemolysin genes. Operon and gene fusions between lacZ and the hemolysin genes, hlyC and hlyA, were performed. These two genes are essential for the synthesis of active hemolysin and are transcribed from a common promoter (p1). Whereas the amount of hemolysin produ...

Negrete, L.M.; Middleton, M.S.; Clark, L.; Wolfson, T.; Gamst, A.C.; Lam, J.; Changchien, C.; Deyoung-Dominguez, I.M.; Hamilton, G.; Loomba, R.; Schwimmer, J.; Sirlin, C.B., 2014: Inter-examination precision of magnitude-based MRI for estimation of segmental hepatic proton density fat fraction in obese subjects. To prospectively describe magnitude-based multi-echo gradient-echo hepatic proton density fat fraction (PDFF) inter-examination precision at 3 Tesla (T). In this prospective, Institutional Review Board-approved, Health Insurance Portability and Ac...

Chen T.R., 1985: Modal karyotype of human leukemia cell line k 562 american type culture collection ccl 243. The chromosome composition of the human leukemic cell line, K562, deposited at the American Type Culture Collection (ATCC) (CCL 243), was studied and further compared with those reported by other laboratories. In CCL 243, the modal karyotype had 5...

James; Chang, 1988: Absorption saturation of heavy- to light-hole band transitions in p-type InSb. Physical Review. B, Condensed Matter 38(14): 9778-9789

Dumont, B.L., 1977: Relationships between conformation and composition of cattle carcasses. 40 carcasses from cows, steers and bulls of various breeds were scored for conformation with the method proposed by the European Association for Animal Production [see ABA 42, 4754]. Carcass composition was estimated by the ratio of muscle + fat w...

Anonymous, 1944: Transplanting of trees and shrubs in the Northeastern and North Central United States. This extremely practical article was prepared for the U.S. Army Camouflage Branch Engineer Board. The whole process of moving big and small trees is considered in great detail and with very clear, explanatory illustrations. Directions and hints ar...

Macleod, A.M.rray; Showell, G.A.drew; Street, L.J.seph, 2001: Aminocyclohexane derivatives as 5-HT receptor agonists. A class of substituted aminocyclohexane derivatives are selective agonists of 5-HT1 -like receptors, being potent agonists of the human 5-HT1D.sup.alpha receptor subtype while possessing at least a 10-fold selective affinity for the 5-HT1D.sup.alp...