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Assessment of eosinophil granule proteins in various body fluids: Is there a relation to clinical variables in childhood asthma?

, : Assessment of eosinophil granule proteins in various body fluids: Is there a relation to clinical variables in childhood asthma?. Clinical & Experimental Allergy 29(6): 786-793, June

Background The eosinophil plays a central role in the inflammatory process in bronchial asthma. Recent studies have indicated that the assessment of eosinophil-derived proteins in various body fluids could be used for monitoring disease activity of childhood asthma. Till now, no study exists which compared the levels of eosinophil-derived proteins in various body fluids such as serum, nasal lavage fluid (NALF) and urine. Objective To investigate whether eosinophil granule proteins in different compartments were correlated and whether there is a relationship betweeen disease activity, pulmonary function and bronchial hyperreactivity. Methods Twenty-eight children with atopic bronchial asthma were recruited. Serum, NALF and urine samples were obtained and assessed for eosinophil cationic protein (ECP) and eosinophil protein X (EPX). The levels of eosinophil proteins were analysed for a relationship with lung function variables, bronchial hyperreactivity and disease activity. Eleven healthy control subjects were used as controls. Results Median ECP and EPX concentrations in serum (31.4 and 74.8 mug/L vs 15.8 and 24.3 mug/L, respectively), NALF (9.9 and 44.9 mug/L vs 0 and 2.5 mug/L, respectively) and urine (49.4 vs 16.5 mug/mmol creatinine) were significantly raised in children with bronchial asthma compared with healthy control subjects. In addition, ECP and EPX levels in serum and urine samples were significantly higher in symptomatic patients compared with asymptomatic subjects with asthma. Although no relationship between eosinophil-derived proteins in serum, NALF or urine and the level of nonspecific bronchial hyperreactivity could be detected, the concentrations of EPX in serum and urine were correlated with variables of pulmonary function. Conclusion Our findings demonstrate increased eosinophil activity in serum, NALF and urine derived from children with bronchial asthma. Due to the relationship between levels of eosinophil proteins in serum/urine samples and lung function, as well as significant concentration differences between symptomatic and asymptomatic asthmatic children, the assessment of eosinophil proteins in serum or urine samples appear to be more appropriate in monitoring disease activity than measurement of ECP or EPX in NALF. Thus, the determination of serum ECP/EPX or urinary EPX may be preferentially used in monitoring eosinophilic inflammation in childhood asthma.


PMID: 10336595

DOI: 10.1046/j.1365-2222.1999.00546.x

Other references

Moreira, D.M.; Bañez, L.L.; Freedland, S.J., 2010: Editorial comment. Journal of Urology 183(5): 1796-1796

Su, Z.Z.; Shi, Y.; Goldstein, N.I.; Fisher, P.B., 1998: Selective expression of the prostate carcinoma tumor antigen gene, PCTA-1, in human prostate carcinoma cell lines and tissues. Proceedings of the American Association for Cancer Research Annual Meeting 39: 414, March

Goldberg, D.; Tan, M.; Dale Sarradet, M.; Gordon, M., 2003: Nonablative dermal remodeling with a 585-nm, 350-microsec, flashlamp pulsed dye laser: clinical and ultrastructural analysis. A nonablative 350-microsec, 585-nm, flashlamp pulsed dye laser is currently being used for the treatment of rhytides. To analyze both clinical rhytid improvement and electron microscopic evidence of ultrastructural changes after treatment with a n...

Dzhamalbekov, E.U.; Kozybaeva, F.E.; Faizullina, A.K., 1994: Results of land reclamation in the arid zone of Kazakhstan. The reclamation of disturbed lands in arid Kazakhstan was studied. It was concluded that: (1) Quaternary loamy and loamy-sand sediments low in humus and nutrients on the Mangyshlak Peninsula are suitable for trees, shrubs and leguminous herbs unde...

Chang, X.; Bian, Y.; Wu, Y.; Huang, Y.; Wang, K.; Duan, T., 2016: Endocan of the maternal placenta tissue is increased in pre-eclampsia. Pre-eclampsia (PE) is associated with intravascular inflammation and endothelial dysfunction. Interestingly, endocan plays a predominant role in the vascular inflammation and is considered as a biomarker of endothelial dysfunction. The aim of this...

Van Oene J.C.; D.V.ies J.B.; Dijkstra D.; Horn A.S., 1986: N n di n propyl 7 hydroxy 2 aminotetralin dp 7 at a selective dopamine autoreceptor agonist. Woodruff, G N , J A Poat And P J Roberts (Ed ) Dopaminergic Systems And Their Regulation; Satellite Symposium Of The Iuphar (International Union Of Pharmacology) 9th International Congress Of Pharmacology, Southampton, England, Uk, August 1984 Xv+501p Vch Verlagsgesellschaft: Weinheim, West Germany (Dist For Continental Europe And Japan By Vch Verlagsgesellschaft: Weinheim, West Germany; Dist For Usa And Canada By Vch Publishers, Deerfield Beach, Florida, Usa) Illus 381-382, 19

Hermelin, T., 1977: Are there genetic consequences from the use of agrochemical substances in the maintenance breeding of cereals?. Bericht 8) 67-76

Moore, W.; Dickens, C., 2012: Doctors in Dickens. Bmj 344: E1193-E1193

Talks, JS., 1979: A survey of natural history of Halvergate Marshes. Report of the Rugby School Natural History Society, 1978- 30-37

Renfordt, E.; Busch, H.; von Cranach, M.; Gulbinat, W.; Tegeler, J., 1983: Particular aspects of the interrater reliability of the AMDP Psychopathology Scale. Modern Problems of Pharmacopsychiatry 20: 125-142