+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Chiral RP-HPLC assay for propafenone enantiomers in rat liver microsomal incubates and its application in vitro metabolism

, : Chiral RP-HPLC assay for propafenone enantiomers in rat liver microsomal incubates and its application in vitro metabolism. Yaoxue Xuebao 35(5): 370-373, May

AIM To separate propafenone enantiomers in rat liver microsomal incubates and investigate probable stereoselectivity in their N-dealkylation. METHODS The concentration of each enantiomer in rat liver microsomal incubates was determined through precolumn derivatization with 2,3,4,6-tetra-O-acetyl-beta-glucopyranosyl isothiocyanate (GITC), followed by RP-HPLC assay. RESULTS A baseline separation of propafenone enantiomers was achieved on C18-ODS column, with methanol - water - glacial acetic acid (67:33:0.05) as mobile phase. The assay was linear from 0.5 to 320 mugcntdotmL-1 for each enantiomer, and the limit of detection was 100 ngcntdotmL-1. The method affords the average recoveries of 77.1% for R-propafenone and 76.0% for S-propafenone. Stereoselectivity was observed for the phase I metabolism of racemic propafenone in dexamethasone, beta-naphthoflavone induced rat liver microsomal incubates, but not in controls. CONCLUSION The method is simple, cheap and can be applied to study the metabolism of R- and S-propafenone in vitro. Stereoselectivity exists in their N-dealkylation.


Other references

Piercy J.W.; Lohr J.M., 1989: Progressive relaxation in the treatment of an elderly patient with insomnia. Clinical Gerontologist 8(4): 3-11

Poletaev, A.B.; Morozov, S.G.; Generalova, G.A.; Krylova, Y.V.; Yakunina, N.A.; Prokudina, O.V., 2000: Human papilloma virus and ontogenetic malformation of central nervous system. The evidences for interdependencies between: 1) HPV infection in pregnant women; 2) anomalous production of natural anti-S100 autoantibodies; and 3) fetal nervous system malformations has been obtained. Based upon experimental data we suppose HPV...

Harris, R.V., 1945: V. Plant pathology. Raspberry Grafting tests of the reaction of new seedling varieties to mosaic are reported. Investigations in Scotland on the raspberry viruses were continued. Strawberry Seedlings and commercial varieties were tested for tolerance to yellow-edge.

Sugrue, M.F.; Funk, H.A.; Lazarides, E.; Mallorga, P.; O'neill Davis, L.A.; Ritter, P.J., 1994: Evidence for a role of membrane bound carbonic anhydrase in intraocular pressure regulation in rabbits. Investigative Ophthalmology & Visual Science 35(4): 1400

Liu, R.; Gutierrez, A.M.; Ring, A.; Persson, A.E.G., 2000: Nitric oxide induces resensitization of P2Y2 nucleotide receptors in cultured rat mesangial cells. Acta Physiologica Scandinavica 170(4): A95-A96, December

Barnes, B.N., 1983: A shipping container for dipteran pupae. A simple container for shipping pupae of Diptera (especially fruit-flies) is described from South Africa. It has its own food and water supplies for adults that emerge in transit, and was tested with Pterandrus rosa (Karsch) and used for Ceratitis...

Santhappa, M., 1980: Present status and future prospects of fur industry in India. Lamb pelt production and processing technology in India: ub 1980) 1-3

de Vonne, T.L.; Versavel, C.; Jousset-Stevenet, B.; Mouray, H., 1977: Isolation of alpha-1-macroglobulin (alpha 1 M) and alpha-2-macroblogulin (alpha 2 M) from rabbit blood. A purification process of rabbit alpha 1 M and alpha 2 M from plasma was described: first the platelets, the fibrinogen, the plasminogen and the low-density lipoproteins were eliminated; then alpha 1 M and alpha 2 M were purified by gel filtration...

Nagar, R.C., 1981: Congenital jejunal diverticulum leading to diverticulitis and volvulus. Journal of the Indian Medical Association 76(7-8): 137-138

Nanjareddy, K.; Blanco, L.; Arthikala, M-Kumar.; Affantrange, X.Alvarado.; Sánchez, F.; Lara, M., 2014: Nitrate regulates rhizobial and mycorrhizal symbiosis in common bean (Phaseolus vulgaris L.). Nitrogen-limited conditions are considered to be a prerequisite for legume-rhizobial symbiosis, but the effects of nitrate-rich conditions on symbiotic status remain poorly understood. We addressed this issue by examining rhizobial (Rhizobim tropi...