geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cloning and characterization of Pseudomonas putida genes encoding the phosphate-specific transport system


, : Cloning and characterization of Pseudomonas putida genes encoding the phosphate-specific transport system. Journal of Bioscience & Bioengineering 87(3): 273-279, March

The pstSCAB genes of Pseudomonas putida PRS2000, encoding the phosphate (Pi)-specific transport (Pst) system, were cloned. The pstS gene of Pseudomonas aeruginosa PAO1, of which the pstCAB genes had been cloned previously, was also cloned (Nikata, T. et al., Mol. Gen. Genet., 250, 692-698, 1996). The predicted translation products of the P. putida pstSCAB genes showed 83, 75, 78 and 88% amino acid identity with their P. aeruginosa counterparts. Two well-conserved Pho box sequences were found in the region upstream of the pstS gene (15/18 base identity with the consensus Pho box sequence) and in the intercistronic region between the pstS and pstC genes (11/18 base identity) of P. putida PRS2000. To investigate the functions of PstSCAB, the pstSC genes were inactivated by inserting a kanamycin resistance gene cassette into the chromosome of P. putida PRS2000. The resultant mutant, designated PNT1, failed to take up 32Pi even under conditions of Pi limitation. Strain PNT1 was also constitutive for alkaline phosphatase synthesis, as well as chemotaxis toward Pi, indicating that the Pst system is involved in the negative regulation of the pho regulon in P. putida. Although overexpression of the pstSCAB genes in P. putida PRS2000 resulted in decreased cell growth, this recombinant strain could remove Pi at a rate similar to that seen with the control strain.

US$19.90

DOI: 10.1016/s1389-1723(99)80031-0


Other references

Linxue, L.; Nohara, R.; Makita, S.; Hosokawa, R.; Hata, T.; Okuda, K.; Hamazaki, H.; Fujita, M.; Sasayama, S., 1999: Effect of long-term exercise training on regional myocardial perfusion changes in patients with coronary artery disease. The cardiac rehabilitation of patients with coronary artery disease (CAD) promotes exercise tolerance, improves left ventricular function, and decreases the heart rate and systolic blood pressure at the same load intensity. Several studies have sh...

Muramatsu I., 1986: Evidence for sympathetic purinergic transmission in the mesenteric artery of the dog. Electrical transmural stimulation of isolated mesenteric artery of the dog produced a transient contraction which consisted of adrenergic and non-adrenergic components. In contrast to the adrenergic component, the nonadrenergic component was resis...

Saner, E.; Nitsch, D.; Descoeudres, C.; Frey, F.J.; Uehlinger, D.E., 2005: Outcome of home haemodialysis patients: a case-cohort study. Background. Randomized, controlled comparisons between home haemodialysis (HHD) and centre haemodialysis (CHD) have not been performed to date. Reported survival benefits of HHD as compared with CHD from uncontrolled studies have been attributed l...

Braniewski, S.; Chrzanowska, E., 1988: Effect of dusts from electro-filters of different industrial works on the vegetation. Numbers of species of mosses, herbaceous plants, shrubs and trees, and stand volume increment, percentage of dead trees, pine crown characteristics and plant element contents were recorded in 1980-84 on plots in forest in Poland dominated by 40-yr...

Wang, J.; Liu, Z.; Chen, C.L.Philip.; Zhang, Y., 2017: Fuzzy Adaptive Compensation Control of Uncertain Stochastic Nonlinear Systems With Actuator Failures and Input Hysteresis. Hysteresis exists ubiquitously in physical actuators. Besides, actuator failures/faults may also occur in practice. Both effects would deteriorate the transient tracking performance, and even trigger instability. In this paper, we consider the pro...

Kober, B.; Müller-Sievers, K.; Schröder, U.G.; Stratmann, H., 1990: A computer-supported method for the direct production of shielding blocks and compensators for radiotherapy. The homogeneous irradiation of irregularly shaped targets with high doses and the simultaneous protection of the equally irregular surrounding high risk regions can be carried out with the help of shielding blocks and compensation filters speciall...

Nilsson, Jytte R., 1993: Effects of a gold salt and its intracellular distribution in Tetrahymena. In studies on effects of heavy metals and drugs containing these, Tetrahymena was exposed to aurothiomalate (GSTM), a drug used in therapy of rheumatoid arthritis. The drug has been reported to affect lysosomal enzymes, to accumulate in lysosomes,...

Harrell, G.T., 1980: The first years of the University of Florida College of Medicine. Journal of the Florida Medical Association 67(3): 247-250

Guillermo Loria, L.; Jimenez, R.; Badilla, M., 2002: Gamma spectrometry analysis of fertilizers used in Costa Rica. The specific activity of natural and artificial isotopes present in national consumption fertilizers, supplied by local dealers in Costa Rica, was quantified using the low level gamma spectrometry technique. The most outstanding isotope found in s...

Keys, A., 1994: 1944 symposium on convalescence and rehabilitation. Faseb Journal 8(14): 1201-1202