geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cloning and determination of S1 gene of IBV GD05 strain and its expression in E. coli


, : Cloning and determination of S1 gene of IBV GD05 strain and its expression in E. coli. Virologica Sinica 17(3): 266-269, August

Acording to Avian Infectious Brochitis virus (IBV) Beaudette strain S1 gene sequence, a pair of primers were designed and synthesized. With the primers, IBV Guangdong isolation strain GD05 S1 gene was successively amplified by RT-PCR. PCR product was digested with BstY I, Hae III and Pst I respectively, the result showed RFLP pattern of was the same as that of M41 S1 gene. IBV GD05 strain was thought as Mass serotype primaryly. IBV GD05 S 1 gene was cloned into pGEM - T vector and sequenced, its sequence was consisted of 1611 base pairs. By comparison, the nucleotide sequence was 97.14% identical to that of IBV M41. IBV GD05 S1 gene was subcloned into expression vector pET21d. SDS-PAGE experiment showed that it expressed in E. coli.

US$29.90


Other references

Nakano, H.; Ashizawa, M.; Akahoshi, Y.; Ugai, T.; Wada, H.; Yamasaki, R.; Ishihara, Y.; Kawamura, K.; Sakamoto, K.; Sato, M.; Terasako-Saito, K.; Kimura, S-Ichi.; Kikuchi, M.; Nakasone, H.; Kako, S.; Kanda, J.; Yamazaki, R.; Tanihara, A.; Nishida, J.; Kanda, Y., 2016: Assessment of the ovarian reserve with anti-Müllerian hormone in women who underwent allogeneic hematopoietic stem cell transplantation using reduced-intensity conditioning regimens or myeloablative regimens with ovarian shielding. Conditioning regimens that include cyclophosphamide (CY) and total body irradiation (TBI) induce severe gonadal toxicity and permanent infertility in approximately 90 % of female patients who undergo hematopoietic stem cell transplantation (HSCT)...

Kasabian A.K.; Colen S.R.; Shaw W.W.; Pachter H.L., 1990: The role of microvascular free flaps in salvaging below amputation stumps a review of 22 cases. Journal of Trauma 30(7): 923

Weston, R.; Brooks, R.; Gladman, J.; Senior, K.; Denley, L.; Silove, D.; Whyman, N.; Kickett, M.; Bryant, R.; Files, J., 2009: Ethical research in partnership with an Indigenous community. The aim of this paper is to describe the implementation of the Community Safety Research Project (CSRP) focusing on violence prevention among Aboriginal communities in western NSW in order to examine how practice converges with contemporary ethica...

Li, Q.; Li, Y.; Xie, Y.; Ren, D., 1995: Expression of chemically synthesized hybrid genes of Plasmodium falciparum and preliminary characterization of expressed products. The chemically synthesized hybrid Plasmodium falciparum genes HGFC and HGFCAC were recombined with the expression vector pWR450-1 and transferred to Escherichia coli. After adding IPTG to the culture medium, bacteria containing the recombinant pla...

Cheng, H.J.ng; Flanagan, J.G., 1994: Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases. Mek4 and Sek are tyrosine kinases with expression patterns in the mouse embryo that suggest important functions In early development. However, like all Eph family kinases, both were identified as orphan receptors without known ligands. We show tha...

Lathe R.; Balland A.; Kohli V.; Lecocq J P., 1982: Fusion of restriction termini using synthetic adapter oligo nucleotides. A method is proposed for linking a DNA fragment possessing a 5' single-stranded extension to one carrying a 3' extension. Synthetic oligonucleotide adaptors can be used to change the site specificity at the termini of a fragment generate...

Hrishi, N.; Marimuthammal, S., 1969: A selective strain to trace pollen tube in Graminaceous style. J. A simple and quick technique is described for tracing the pollen tube inside the style of sugar cane by rendering the surrounding stylar tissue colourless in contrast to the deep-purple stained pollen tube. The technique works satisfactorily with...

Burdzik, W., 1999: Flexible shear connections in composite construction - a spring model. A method is presented whereby the bending and axial stiffness of composite members may be calculated if the stiffness of the connectors is known.

Sewerin, I., 1983: From cathode-tube to computer tomography. Tandlaegebladet 87(11): 383-388

Kendrick, A.; Lyver, P.O.b; Lutsel-Ke-Dene-First-Nation, 2005: Denesoline (Chipewyan) knowledge of barren-ground caribou (Rangifer tarandus groenlandicus) movements. Semi-directed interviews relating to the traditional knowledge (TK) of barren-ground caribou (Rangifer tarandus groenlandicus) movements were conducted with elders and hunters from the Denesqline (Chipewyan) community of Lutsel K'e, Northwest...