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Cloning and expression of a new Tibetan hulless barley (Hordeum vulgare) beta-1,3-glucanase gene


, : Cloning and expression of a new Tibetan hulless barley (Hordeum vulgare) beta-1,3-glucanase gene. Biotechnology Letters 25(8): 617-622

A new full-length beta-1,3-glucanase cDNA was obtained by RT-PCR and RACE techniques from Tibet hulless barley and its complete gene sequence obtained by DNA Walking. Sequence alignment with the BLAST program showed that cDNA has high similarity with barley beta-1,3-glucanase II. The gene was functionally expressed in E. coli and the recombinant protein catalysed the hydrolysis of beta-1,3-glucan with an action pattern characteristic of a beta-1,3-glucan endohydrolase (EC 3.2.1.39). Southern blot analysis indicated that the gene is a member of a small gene family. RT-PCR and northern blot analysis indicated it is constitutively expressed in barley shoots.

US$29.90

PMID: 12882155


Other references

M.Hong ching, 1982: The spatial distribution of the b-values before large and moderate earthquakes. Diqiu Wulixue Bao = Acta Geophysica Sinica 25(2): 163-171

Werner John; Stein Seth; Engelin Joseph F., 1986: Kinematic models for microplate evolution. Eos, Transactions, American Geophysical Union 67(16): 361

Skirkevicius, A., 1984: Behavior of worker honeybees (Apis mellifera L.) after licking quenn pheromones. Khemoretseptsiia nasekomykh = Insect chemoreception: ) 103-117

Gertner, J.M.; Tamborlane, W.V.; Hintz, R.L.; Horst, R.L.; Genel, M., 1981: The effects on mineral metabolism of overnight growth hormone infusion in growth hormone deficiency. To determine whether alterations in mineral handling induced by acute GH [growth hormone] infusion were associated with changes in vitamin D metabolism or parathyroid function, 11 GH-deficient patients were studied. Basal serum Ca, P, glucose, ins...

Campbell, A.H., 1934: Zone lines in plant tissues. II. The black lines formed by Armillaria mellea (Vahl) Quel. The results of the investigation described in some detail in this paper showed that, as in Xylaria polymorpha, the black lines formed in wood infected by Armillaria mellea are the sectional outlines of closed bodies, enclosing an extensive matrix...

Villegas-Borrel, J.L.; Pascual-Góngora, C.; Ramírez-Velasco, J.A.; Villalpando-Canchola, R., 1987: Medico-surgical management of vesicoureteral reflux. Boletin Medico del Hospital Infantil de Mexico 44(3): 180-183

Inaba, T.; Nagano, Y.; Sakakibara, T.; Sasaki, Y., 1999: Identification of a cis-regulatory element involved in phytochrome down-regulated expression of the pea small GTPase gene pra2. The pra2 gene encodes a pea (Pisum sativum) small GTPase belonging to the YPT/rab family, and its expression is down-regulated by light, mediated by phytochrome. We have isolated and characterized a genomic clone of this gene and constructed a fus...

Kissick, D.J.; Wanapun, D.; Simpson, G.J., 2011: Second-order nonlinear optical imaging of chiral crystals. Second-order nonlinear optical imaging of chiral crystals (SONICC) is an emerging technique for crystal imaging and characterization. We provide a brief overview of the origin of second harmonic generation signals in SONICC and discuss recent stud...

Liu, S.Q.ng; Chen, Y.; Chen, H.Y.an, 2002: Electrochemical studies on a zirconium hexacyanoferrate modified electrode and utility for the determination of rubidium. Zirconium hexacyanoferrate (ZrHCF), a new Prussian blue analogue, was immobilized on the surface of a gold electrode by potential scan. The results showed that the peak current is proportional to scan rate up to 200 mVs-1, showing the surface reac...

Eskildsen, J., 1985: Is it a new era for storks in Denmark?. Fugle, 55: 17-18