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Cloning and expression of curcin, a ribosome-inactivating protein from the seeds of Jatropha curcas


, : Cloning and expression of curcin, a ribosome-inactivating protein from the seeds of Jatropha curcas. Acta Botanica Sinica 45(7): 858-863, July

Curcin, a ribosome-inactivating protein with a molecular weight of about 28.2 kD, which strongly inhibits the protein synthesis in rabbit reticulocyte lysate system with an IC50 value of about (0.19+-0.01) nmol/L, was purified from the seeds of Jatropha curcas L. The protein has the activity of rRNA N-glycosidase. Degenerate primers were designed based on the N-terminal partial sequence from purified curcin. The full-length curcin cDNA by RT-PCR and 5'-RACE was cloned. The deduced amino acids sequence indicates that a preprotein with 293 amino acid residues is first translated and then processed to a mature protein with 251 amino acids. The deduced amino acids sequence shares homology of 33% and 57% to those of type I ribosome-inactivating proteins (RIPs) and A chain of type II RIPs, respectively. The sequence encoding mature curcin was integrated into the pQE-30 vector for expression in Escherichia coli strain M15 (pREP4). The purified recombinant curcin was able to inhibit protein synthesis in rabbit reticulocyte lysate system.

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Other references

Roetert E.P.; Garrett G.E.; Brown S.W.; Camaione D.N., 1991: Development of a performance profile to assess nationally ranked junior tennis players. Medicine & Science in Sports & Exercise 23(4 SUPPL): S42

Heard, I.; Tassie, J-Michel.; Kazatchkine, M.D.; Orth, Gérard., 2002: Highly active antiretroviral therapy enhances regression of cervical intraepithelial neoplasia in HIV-seropositive women. Objective: This study was undertaken to investigate the impact of highly active antiretroviral therapy (HAART) on the regression of cervical intraepithelial neoplasia (CIN) in HIV-infected women. Design: Prospective study of 168 HIV-infected women...

Botella Llusia, J.; Del Tornero, M.C.; Fernandez Sanchez, L., 1955: Intermediary metabolism of androgens in the normal woman. III. Report. Archivos de Medicina Experimental; Trabajos del Instituto Nacional de Ciencias Medicas 18(2): 145-156

Favre, M.; Goldmann, H., 1956: Genesis of posterior vitreus body detachment. Ophthalmologica. Journal International D'ophtalmologie. International Journal of Ophthalmology. Zeitschrift für Augenheilkunde 132(2): 87-97

Brodie, I.A.; Denham, R.A., 1974: The treatment of unstable ankle fractures. Journal of Bone and Joint Surgery. British Volume 56(2): 256-262

Sadettanh, K.; Kumar, S., 2004: Renewable energy resources potential in Lao PDR. Renewable energy resources provide a large share of the total energy consumption of many developing countries. Evaluation of these resources has not been made in many countries and in many cases, data necessary to estimate the resource availabilit...

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Förderreuther, S., 2004: Symptomatic headache. Essential differential diagnosis. A patient is suspected of suffering from a potentially life threatening headache according to the following typical warning signs: sudden onset headache, worst headache, neurological findings, fever, neck stiffness, epileptic seizures and deterior...

Masner, P.; Sláma, K.; Zdárek, J.; Landa, V., 1970: Natural and synthetic materials with insect hormone activity. X. A method of sexually spread insect sterility. The following is virtually the authors' abstract. Substances with juvenile hormone activity appear to possess the characteristics of ideal chemosterilants for insects. During investigations in Czechoslovakia on the sexual spread of sterility,...

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