geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cloning, expression, and characterization of human metalloprotease 1: a novel member of the pitrilysin family of metalloendoproteases


, : Cloning, expression, and characterization of human metalloprotease 1: a novel member of the pitrilysin family of metalloendoproteases. Dna and Cell Biology 18(5): 369-380

A novel cDNA, designated human metalloendoprotease 1 (hMP1), was identified on the basis of homology to known metalloendoproteases of the pitrilysin family. The full-length MP1 codes for a protein with an open reading frame of 1038 amino acids. The N-terminal region contains the HXXEH(X)76E catalytic domain that is conserved in the members of pitrilysin family, namely insulin-degrading enzyme and NRD convertase. The hMP1 mRNA is expressed in a number of cell lines and tissues as a single species of about 3.4 kb. The expression of hMP1 mRNA is higher in muscle and heart than in brain, pancreas, liver, lung, and placenta. The full-length hMP1 was expressed in the baculovirus system and purified to homogeneity using isoelectrofocusing and ion-exchange chromatography. The enzyme exhibited a neutral pH optimum and high sensitivity to thiol reagents. HMP1 was inactivated by 1,10-phenanthroline, a specific inhibitor of Zn(+2)-dependent metalloproteases. The enzyme was not inhibited by agents that inhibit neutral metalloendoproteases of the thermolysin family such as thimet endo-oligopeptidase, enkephalinase, or angiotensin-converting enzyme. HMP1 cleaved a prodynorphin-derived peptide, leumorphin, N-terminal to Arg in the monobasic processing site, as evidenced by MALDI-TOF mass spectrometry. However, the enzyme did not exhibit strict monobasic cleavage specificity, as peptide substrates with amino acid substitutions around the monobasic site was cleaved efficiently by hMP1. Taken together, these results suggest that hMP1 is a novel member of the metalloendoprotease superfamily with ubiquitous distribution that could play a broad role in general cellular regulation.

US$19.90

PMID: 10360838

DOI: 10.1089/104454999315268


Other references

ten Duis, H.J., 1995: Acute electrical burns. Electric current can damage an individual by thermal heating of the tissues; by disregulating autonomously functioning organ systems, such as the circulatory and respiratory systems; or by once-only or continuing stimulation of the nerves and stri...

Fox, M., 1993: Vasectomy reversal by microsurgery. Approximately 90,000 vasectomies are carried out in the United Kingdom every year, and in 1982 it was reported that 3% of vasectomized men were asking for reversal. Vasectomy reversal (vasovasostomy) can be achieved in some cases by approximating...

Kyank, H., 1957: Body temperature of the newborn in normal and pathological pregnancy. Archiv für Gynakologie 189: 213-217

Blangero, A.; Ota, H.; Delporte, L.; Revol, P.; Vindras, P.; Rode, G.; Boisson, D.; Vighetto, A.; Rossetti, Y.; Pisella, L., 2007: Optic ataxia is not only 'optic': impaired spatial integration of proprioceptive information. Optic ataxia is considered to be a specific visuo-manual guidance deficit, which combines pointing errors due to the use of the contralesional hand ("hand effect") and to the presentation of the visual target in the contralesional field...

Wang, L.; D.L.cia, G.; Weinmann, S.M., 2013: On the scatter in the relation between stellar mass and halo mass random or halo formation time dependent?. The empirical traditional halo occupation distribution (HOD) model of Wang et al. fits, by construction, both the stellar mass function and correlation function of galaxies in the local Universe. In contrast, the semi-analytical models of De Lucia...

Torday J.S.; Hua J.; Lamont C.; Rubin L., 1991: The role of cyclic amp as a mediator of glucocorticoid stimulated fetal rat lung fibroblast maturation. Pediatric Research 29(4 PART 2): 53A

Guagliardo, V.; Bouhnik, A-D.; Verger, P., 2007: Evaluation of vaccine coverage in children from 2 to 4 years old in South-Eastern France. Few studies have evaluated vaccination coverage in early childhood in South-Eastern France and spatial and social factors associated to it. We carried out a study in children aged 3.5-4.5 years and attending 112 nursery schools randomly selected i...

Forasiepi, A.M.; Martinelli, A.G.; Goin, F.J., 2007: Taxonomic revision of Parahyaenodon argentinus Ameghino and its implicances for the knowledge of the Mio-Pliocene large carnivorous mammals of South America Revision taxonomica de Parahyaenodon argentinus Ameghino y sus implicancias en el conocimiento de los grandes mamiferos carnivoros del Mio-Plioceno de America de Sur. Parahyaenodon argentinus, from the Late Miocene-Early Pliocene of Monte Hermoso (Buenos Aires Province, Argentina), was recognized by Ameghino in 1904, who regarded it as Hyaenodontidae (Eutheria, Creodonta). Based on its dental features, later au...

Anonymous, 1988: Spanish wine. Horticultural products review FHORT US Department of Agriculture Foreign Agricultural Service: (8-88) 19-21

Sánchez-Burgos, G.; Ramos-Castañeda, Jé.; Cedillo-Rivera, R.; Dumonteil, E., 2010: Immunogenicity of novel Dengue virus epitopes identified by bioinformatic analysis. We used T cell epitope prediction tools to identify epitopes from Dengue virus polyprotein sequences, and evaluated in vivo and in vitro the immunogenicity and antigenicity of the corresponding synthetic vaccine candidates. Twenty-two epitopes wer...