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Comparison of the growth and hydrogen peroxide production by vaginal probiotic lactobacilli under different culture conditions


, : Comparison of the growth and hydrogen peroxide production by vaginal probiotic lactobacilli under different culture conditions. American Journal of Obstetrics & Gynecology 188(1): 35-44, January

OBJECTIVE: The purpose of this study was to determine the optimal conditions for the growth and hydrogen peroxide production by vaginal lactobacilli. STUDY DESIGN: Two vaginal lactobacillus strains were cultured under different growth conditions (temperature, pH, agitation, and growth media) with a fractional factorial experimental design. RESULTS: The optimal growth conditions of Lactobacillus paracasei CRL (Centro de Referencia para Lactobacilus Culture Collection) 1289 and Lactobacillus crispatus CRL 1266 were temperature 37degreeC and initial pH 6.5, nonagitated cultures, in autolysat de levure; peptone, tryptone, Tween 80 et glucose (LAPTg) broth for L paracasei or in de Man-Rogosa-Sharpe (MRS) and LAPTg broths for L crispatus. The hydrogen peroxide production, detected only in agitated cultures, was higher at 37degreeC and pH 6.5. The oxidative metabolite produced the self-inhibition of the lactobacilli growth. In mixed cultures of lactobacilli and Staphylococcus aureus under the optimal conditions of hydrogen peroxide production, the pathogen growth was inhibited. CONCLUSION: The results provided information about the factors that affect the hydrogen peroxide production and about the most favorable conditions with which to obtain the highest biomass in the shortest possible time.

US$19.90

PMID: 12548193

DOI: 10.1067/mob.2003.123


Other references

Morikawa, T.; Tanaka, T.; Tatsuno, K.; Imamura, E.; Imai, Y., 1973: Radical surgery in transposition of great vessels--Rastelli operation. Kyobu Geka. Japanese Journal of Thoracic Surgery 26(9): 609-618

Mattei J.; Combemorel R.; Enay R., 1987: Presence of middle liassic atractites aulacoceratida in the causses southern massif central france. Various Middle Liassic (essentially Domerian) biosedimentary sites of the Causses yield phragmocone elements (17 in total) of Atractites. Their presence had been mentioned (without description) by Mattei (1971, 1985) for the first time in this reg...

Prakken, R., 1942: A spontaneous haploid of Nicotiana Tabacum. The cytology of a spontaneous haploid of N. Tabacum is described. Up to two bivalents may be produced, also occasional restitution nuclei. The significance of these results for the question of the presumed allopolyploid origin of the species is di...

Wagner, H., 1953: Functional thoughts on therapy of leukorrhea. Therapie der Gegenwart 92(10): 371-377

Jaspers, A.W.; Cox, J.L.; Krumholz, H.M., 2017: Copy Fees and Limitation of Patients' Access to Their Own Medical Records. JAMA Internal Medicine 177(4): 457-458

Anonymous, 2012: Brainwave training boosts network for cognitive control and affects mind-wandering. A breakthrough study conducted at Western University, Canada, and Lawson Health Research Institute has found that training of the well-known brainwave in humans, the alpha rhythm, enhances a brain network responsible for cognitive-control. The tra...

Hofer, S.; Andersson, R.; Sommer, H., 1993: Milk screening tests for herd health controlling and for milk quality determination. The BIPS (Bonner Information and Preventive System) herd control programme for cattle is aimed at the early detection of risk factors for mastitis, and their elimination. This chapter deals only with screening tests for udder health and metabolic...

Meador, W.E.; Quiocho, F.A., 1978: Preliminary crystallographic data for a leucine, isoleucine, valine-binding protein from Escherichia coli K12. Journal of Molecular Biology 123(3): 499-502

Macedo, R.S.; Lombardi, A.T.; Omachi, C.Y.; Rörig, L.R., 2008: Effects of the herbicide bentazon on growth and photosystem II maximum quantum yield of the marine diatom Skeletonema costatum. Chlorophyll fluorescence techniques are used for the detection of toxic substances in samples of photosynthetic cells by measuring chlorophyll a fluorimetric parameters, which are a response of the PSII physiological status. This work was conducte...

Kjellgren, O.; Motarjeme, A.; Feld, S.; Mishkel, D.C.; Underwood, C.; Kirkeeide, R.L.; Smalling, R.W., 1996: Rotational atherectomy with a new device: initial clinical experience. The Bard Atherectomy Catheter is a new rotational atherectomy device that consists of a flexible, hollow, thin-walled cutting catheter that, while rotated at 1,500 revolutions per minute, is advanced across the lesion over a special spiral guidewi...