+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Computational and experimental investigation of flow and particle settling in a roller bottle bioreactor

, : Computational and experimental investigation of flow and particle settling in a roller bottle bioreactor. Biotechnology & Bioengineering 63(2): 185-196, April 20

It is shown that cell settling is a key factor affecting the performance of roller bottle bioreactors. The two-dimensional cross-sectional flow at the center of a roller bottle is simulated using a finite difference method, and the settling behavior of cells is simulated using particle dynamics algorithms and validated experimentally using fluorescent particles. The settling behavior of particles in the roller bottle flow is studied using both steady and time dependent rotation rates. Under steady flow conditions the flow is divided into two regions: one where the particles settle to the wall and one where the particles remain suspended indefinitely. The relative size of these two regions depends on the ratio of the settling velocity to the rotation rate of the bottle. For unsteady flows generated by periodic changes of the bottle rotation direction, the settling of cells is accelerated significantly, leading to complete deposition in just a few turns of the bottle.


PMID: 10099595

DOI: 10.1002/(sici)1097-0290(19990420)63:2<185::aid-bit7>;2-c

Other references

Mahler, AE., 1984: Thermoregulatory posturing in a population of Argiope argentata. American Arachnology, 12 No. 30

Collins, HL., 1972: Mouth brooding behavior in the substrate spawning cichlid, Tilapia sparrmani. Journal Minn Acad Sci, 381: 17-18

Resch G.; Simpson C.W., 1989: Thermogenic stimuli elicit autonomic and behavioral responses from the same heat gain site in rats. FASEB Journal 3(3): A531

Kiss, E.; Baji, Ió.; Mayer, László.; Skultéti, Dóra.; Benák, Ián.; Vetró, A., 2007: Validity and psychometric properties of a quality of life questionnaire in a Hungarian child and adolescent population. The study of the quality of life in various strata of society is a very important task. No quality of life research has been done on the Hungarian child population until now. In the present study, we assessed the life quality in a sample of elemen...

Brown W.E.; Chow L.C., 1981: Effects of neutral salts on caries mechanisms. Journal of Dental Research 60(SPEC ISSUE A): 382

Ucisik, A.Suheyl.; Trapp, S.; Kusk, K.Ole., 2007: Uptake, accumulation, phytotoxicity, and removal of 2,4-dichlorophenol in willow trees. The relation between uptake, accumulation, toxicity and removal of 2,4-dichlorophenol (2,4-DCP) in willow trees (Salix viminalis) and the feasibility of implementing phytoremediation as a treatment method for 2,4-DCP contamination in wastewater a...

Rubiales, D.; Ballesteros, J.; Martin, A., 1991: The reaction of X Tritordeum and its Triticum spp. and Hordeum chilense parents to rust diseases. Hexaploid and octoploid tritordeums and their parents Hordeum chilense and Triticum spp. were screened for resistance to isolates of wheat and barley yellow and brown rusts. All H. chilense lines were highly resistant to both wheat and barley brow...

Enright, J.T., 1996: Sequential stereopsis: A simple demonstration. Most people strongly prefer to use back-and-forth eye movements in order to discriminate 3-dimensional distances among targets that are widely separated from each other in direction. This viewing strategy permits sequential stereopsis: a compariso...

Kang, E.; Rapkin, B.D.; DeAlmeida, C., 2006: Are psychological consequences of stigma enduring or transitory? A longitudinal study of HIV stigma and distress among Asians and Pacific Islanders living with HIV illness. Cross-sectional findings have shed considerable light on the relationships between illness stigma and psychological outcomes among persons living with HIV/AIDS in the United States. However, no studies have examined the possible long-term conseque...

Hamre, K.M.; Tharp, R.; Fernandez Gonzalez, A.; Smeyne, R.J., 1997: The role of genotype and sex on MPTP-induced degeneration in the substantia nigra of mice. Society for Neuroscience Abstracts 23(1-2): 1654