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Contact fluorescence imaging of reentry in monolayers of cultured neonatal rat ventricular myocytes


, : Contact fluorescence imaging of reentry in monolayers of cultured neonatal rat ventricular myocytes. Journal of Cardiovascular Electrophysiology 11(6): 665-676, June

Introduction: We present a novel contact fluorescence imaging (CFI) approach to monitor transmembrane potentials in monolayers of cultured neonatal rat ventricular cells. We apply CFI to demonstrate, for the first time, long-term recordings as well as electrical induction and termination of reentrant activity in this in vitro model. Methods and Results: CFI was performed in confluent cell monolayers stained with di-8-ANEPPS. An anatomic obstacle (6 X 0.5 mm) was created in the center of the monolayers. Reentry was induced with a premature stimulus after pacing at 2 Hz (both via field stimulation). Seven sustained (>3 min) reentrant episodes, anchored to the anatomic obstacle, were observed in three monolayers. Field stimulation (30 V/cm) was applied to successfully terminate 6 of the 7 reentries. Analysis of reentrant activity showed similarities with anatomic reentry in tissue preparations, such as reduced conduction velocity around the core, variable conduction velocity along the reentrant pathway due to wavefront curvature effects, and field-induced activation at the obstacle borders leading to reentry termination (cardioversion). Conclusion: This study demonstrates the feasibility of CFI for macroscopic optical mapping of transmembrane potentials in a single layer of cultured cells. Our results suggest that the monolayer cell culture model is an attractive complement to tissue models of reentry and cardioversion.

US$19.90

PMID: 10868740

DOI: 10.1111/j.1540-8167.2000.tb00029.x


Other references

Weski, O., 1953: In memoriam Oskar Weski. Deutsche Stomatologie 3(2): 33-33

Fisher A.B.; Chander A.; Dodia C.; Reicherter J., 1988: Uptake of protein and lipid components of natural surfactant by granular pneumocytes. Journal of Cell Biology 107(6 PART 3): 351A

Barnett, P.G.; Chow, A.; Joyce, V.R.; Bayoumi, A.M.; Griffin, S.C.; Nosyk, B.; Holodniy, M.; Brown, S.T.; Sculpher, M.; Anis, A.H.; Owens, D.K., 2011: Determinants of the cost of health services used by veterans with HIV. The effect of adherence, treatment failure, and comorbidities on the cost of HIV care is not well understood. To characterize the cost of HIV care including combination antiretroviral treatment (ART). Observational study of administrative data. To...

Zipper, J., 1968: Striated muscles in the brain. Zentralblatt für Allgemeine Pathologie U. Pathologische Anatomie 111(1): 21-23

Misiewicz, J.; Afonin, S.; Grage, S.L.; van den Berg, J.; Strandberg, E.; Wadhwani, P.; Ulrich, A.S., 2016: Action of the multifunctional peptide BP100 on native biomembranes examined by solid-state NMR. Membrane composition is a key factor that regulates the destructive activity of antimicrobial peptides and the non-leaky permeation of cell penetrating peptides in vivo. Hence, the choice of model membrane is a crucial aspect in NMR studies and sh...

Engel, W.K., 2001: In type-2 diabetes , diabetic neuropathy and diabeto-2-genetic neuropathy are often dysimmune and respond to intravenous IgG treatment. "Diabetic neuropathy" is commonly considered metabolic or ischemic and untreatable (Griffin '00). We previously found (Engel and Prentice '93, Engel '97) that the neuropathy in most of our D2 patients responds to antidysim...

Ives J.D., 1962: Iron mining in permafrost, central Labrador-Ungava. The decision to undertake large-scale mining of Fe ore in the vicinity of Schefferville was partly based upon the proving of 400 million tons of ore reserves. This essential economic consideration set in motion one of the largest commercial develo...

Sathasivan, K.; Haughn, G.W.; Murai, N., 1990: Nucleotide sequence of a mutant acetolactate synthase gene from an imidazolinone-resistant Arabidopsis thaliana var. Columbia. Acetolactate synthase (ALS) is the key enzyme in the biosynthesis of leucine, isoleucine and valine. ALS is inhibited by 3 groups of herbicides: imidazolinones, sulfonylureas and triazolo pyrimidines. The mutant plant and its ALS are 100 times mor...

Anonymous, 2007: Wanted: studies on mortality estimation methods for humanitarian emergencies, suggestions for future research. Measuring rates and circumstances of population mortality (in particular crude and under-5 year mortality rates) is essential to evidence-based humanitarian relief interventions. Because prospective vital event registration is absent or deteriorat...

Dustan, G.G.; Putman, W.L., 1949: Particle Size of DDT wettable Powders in Relation to Toxicity. The relative toxicity of three wettable spray powders that contained 50 per cent. DDT, but differed in average particle size as a result of the processing methods involved, was tested in the laboratory and greenhouse in Ontario. They comprised mic...