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Correlative microscopy of ultrathin cryosections is a powerful tool for placental research

, : Correlative microscopy of ultrathin cryosections is a powerful tool for placental research. Placenta 24(5): 557-565, May

In this report, we describe procedures for correlative fluorescence and electron microscopy in immunocytochemical studies on the human placenta. Ultrathin cryosections of placenta were used for detection of the distribution of antigens by immunofluorescence and subsequently by immunoelectron microscopy of the same ultrathin cryosection. This methodology has certain advantages over conventional immunohistochemistry and immunoelectron microscopy. The advantages are, most notably, that the same exact structures are examined by both imaging modalities. In addition, since the tissue is physically sectioned (50-100 nm thickness), greater resolution for fluorescence can be obtained in the z-dimension than can be obtained by optical sectioning in confocal microscopy. This last point is of particular importance for discriminating between structures closely stacked in the z-dimension. In this report, we have determined the distribution of caveolin-1 in ultrathin cryosections of terminal villi of the human term placenta. We demonstrate that the use of ultrathin cryosections is a powerful approach for immunofluorescence and correlative microscopy for the in situ localization of antigens.


PMID: 12744933

DOI: 10.1053/plac.2002.0942

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Suc, J.-Pierre; Bertini, A.; Combourieu-Nedbout, N.; Diniz, F.; Leroy, S.; Russo-Ermolli, E.; Zheng, Z.; Bessais, E.; Ferrier, J., 1995: Structure of West Mediterranean vegetation and climate since 5.3 ma. Pollen analyses of 31 selected localities from the West Mediterranean region, spanning from the Early Pliocene to the present, are discussed on the basis of latitude, altitude and physiography. Northwestern and southwestern vegetation provinces ar...

Shang, C., 2001: The future of integrative medicine. Archives of Internal Medicine 161(4): 613-614

Ganz P.A., 1988: Patient education as a moderator of psychological distress. Journal Of Psychosocial Oncology: 181-197

Webb Fred Jr.; Raymond Loren A., 1989: Evaporite texture in Knox Group chert and the Knox-Middle Ordovician unconformity, Nebo Quadrangle, Virginia; implications for the Ordovician history of southwestern Virginia. Abstracts with Programs - Geological Society of America 21(3): 64

Burstedde V.; Scharf R.E.; Kind P.; Goerz G.; Wehmeier A.; Schneider W., 1986: Platelet secretion and thrombin activity in patients with progressive sclerosis pss. Haemostasis 16(SUPPL 5): 55-56

Struve, R., 1942: Aphidon dor Nordseeinsel Borkum. Arb Physiol Angew Ent Berlin-Dahlem, 9: 51-54

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