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Crystallization and preliminary X-ray crystallographic analysis of NAD+-dependent DNA ligase from Thermus filiformis

, : Crystallization and preliminary X-ray crystallographic analysis of NAD+-dependent DNA ligase from Thermus filiformis. Acta Crystallographica Section D Biological Crystallography 56(3): 357-358, March

A highly thermostable DNA ligase from Thermus filiformis has been crystallized at room temperature using methoxypolyethylene glycol 5000 as a precipitant. The crystal belongs to the monoclinic space group P2(1), with unit-cell parameters a = 90.63, b = 117.80, c = 98. 65 A, beta = 115.56 degrees. Two molecules of DNA ligase are present in the asymmetric unit, giving a crystal volume per protein mass (V(m)) of 3.1 A(3) Da(-1) and a solvent content of 61%. A native data set extending to 3.0 A resolution has been collected at 100 K using synchrotron X-rays.


PMID: 10713526

DOI: 10.1107/s090744490000010x

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Marks, D.M.; Han, C.; Krulewicz, S.; Pae, C-Un.; Peindl, K.; Patkar, A.A.; Masand, P.S., 2009: History of depressive and anxiety disorders and paroxetine response in patients with irritable bowel syndrome: post hoc analysis from a placebo-controlled study. Primary Care Companion to the Journal of Clinical Psychiatry 10(5): 368-375

Buckwalter, J.A.; Mower, D.; Schaeffer, J., 1987: Differences in matrix vesicle concentration among growth plate zones. We studied the proximal tibial growth plates of 15-day-old mice to determine if matrix vesicle concentration varies among growth plate zones or between the pericellular and territorial matrix compartment and the interterritorial matrix compartment...

Takadera, T.; Leung, S.; Gernone, A.; Koga, Y.; Takihara, Y.; Miyamoto, N.G.; Mak, T.W., 1989: Structure of the two promoters of the human lck gene: differential accumulation of two classes of lck transcripts in T cells. The human T-cell- or lymphocyte-specific gene, lck, encodes a tyrosine kinase and is a member of the src family. In this report we demonstrate that there are two classes of human lck transcripts (types I and II), containing different 5'-untra...

Sapienza, C., 1990: Genome imprinting, cellular mosaicism and carcinogenesis. Molecular Carcinogenesis 3(3): 118-121

Wang, Y.Z.; Miller, J.D., 1989: Study on the resistance of wheat cultivars to the mycotoxin produced by Fusarium graminearum. An etiolated wheat coleoptile bioassay was used to determine the effects of 7 mycotoxins produced by F. graminearum [Gibberella zeae] on 14 spring and 7 winter wheat cultivars at 10-3 to 10-6 M. The mycotoxins were: deoxynivalenol [vomitoxin], 3-a...

Villeneuve, J.P.; Rocheleau, F.; Raymond, G., 1984: Triamterene kinetics and dynamics in cirrhosis. Triamterene is extensively metabolized by the liver and undergoes important presystemic elimination in normal subjects after oral doses. We examined triamterene disposition in eight healthy controls and seven patients with cirrhosis and ascites. A...

Leon, J.; Ibarra, G.; Acosta, N.; Flores, M., 1984: Nutritive value of king grass. I. Bromatological composition and in vitro digestibility. Pennisetum purpureum X P. americanum was sown on a greyish Pardo soil, a cut taken immediately it was established and the forage was examined 30, 45 and 60 days later. DM, ash and in vitro digestibility decreased with increasing age (P< 0.05) b...

Rehman, A.; Nijhof, A.M.; Sauter-Louis, C.; Schauer, B.; Staubach, C.; Conraths, F.J., 2017: Distribution of ticks infesting ruminants and risk factors associated with high tick prevalence in livestock farms in the semi-arid and arid agro-ecological zones of Pakistan. Tick infestation is the major problem for animal health that causes substantial economic losses, particularly in tropical and subtropical countries. To better understand the spatial distribution of tick species and risk factors associated with tic...

Holowiecki, J.; Wojnar, J.; Krawczyk, M.; Kruzel, T.; Giebel, S.; Kachel, L.; Wojciechowska, M.; Holowiecka, B.; Jagoda, K., 1998: ABMT using bone marrow stored at 4 degrees C and the CAV conditioning regimen Promising results in adult ALL. Blood 92(10 SUPPL 1 PART 1-2): 366B, Nov 15

Rank, G.H.; Bech-Hansen, N.T., 1973: Single nuclear gene inherited cross resistance and collateral sensitivity to 17 inhibitors of mitochondrial function in S. cerevisiae. Molecular & General Genetics 126(2): 93-102