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Crystallization and preliminary X-ray crystallographic analysis of NAD+-dependent DNA ligase from Thermus filiformis


, : Crystallization and preliminary X-ray crystallographic analysis of NAD+-dependent DNA ligase from Thermus filiformis. Acta Crystallographica Section D Biological Crystallography 56(3): 357-358, March

A highly thermostable DNA ligase from Thermus filiformis has been crystallized at room temperature using methoxypolyethylene glycol 5000 as a precipitant. The crystal belongs to the monoclinic space group P2(1), with unit-cell parameters a = 90.63, b = 117.80, c = 98. 65 A, beta = 115.56 degrees. Two molecules of DNA ligase are present in the asymmetric unit, giving a crystal volume per protein mass (V(m)) of 3.1 A(3) Da(-1) and a solvent content of 61%. A native data set extending to 3.0 A resolution has been collected at 100 K using synchrotron X-rays.

US$19.90

PMID: 10713526

DOI: 10.1107/s090744490000010x


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