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Cyclooxygenase 2 expression in Barrett's esophagus and adenocarcinoma: Ex vivo induction by bile salts and acid exposure


, : Cyclooxygenase 2 expression in Barrett's esophagus and adenocarcinoma: Ex vivo induction by bile salts and acid exposure. Gastroenterology 10(5): 487-496, March 9

Background and Aims: Barrett's esophagus (BE) results from chronic, severe gastroesophageal reflux and pre-disposes to esophageal adenocarcinoma. Cyclooxygenase (COX)-2 is involved in chronic inflammation and epithelial cell growth. We investigated COX-2 expression in BE and esophageal adenocarcinoma to explore a potential relation between COX-2 expression and metaplasia or carcinogenesis. Methods: Endoscopic mucosal biopsy specimens of Barrett's intestinal metaplasia (n = 30), Barrett's dysplasia (n = 11), and esophageal adenocarcinoma (n = 5) were compared with normal esophagus (n = 46) and duodenum (n = 46) and analyzed by Western blotting and immunohistochemistry. Results: Immunoblots revealed constitutive expression of COX-2 in normal esophagus and duodenum. COX-2 protein expression was significantly higher in patients with Barrett's metaplasia, dysplasia, and adenocarcinoma compared with normal squamous esophageal or columnar duodenal epithelia and was heterogenous in different regions of the BE surface. Immunohistochemistry revealed prominent staining in the glands of BE, dysplasia, and adenocarcinoma and faint staining in the basal layers of squamous esophagus and the surface of the duodenum. In response to pulses of acid or bile salts in an ex vivo organ culture system, COX-2 expression increased significantly in BE tissues, and this effect was attenuated by the selective COX-2 inhibitor NS-398. Conclusions: The results show COX-2 expression in normal esophagus, which increases significantly in BE and esophageal adenocarcinoma. COX-2 is regulated ex vivo by exposure to acid or bile salts.

US$19.90

PMID: 10702199

DOI: 10.1016/s0016-5085(00)70254-x


Other references

Kaku, R., 1976: Experimental evaluation of assisted extracorporeal circulation using Landé-Edwards membrane oxygenator for induced acute pulmonary insufficiency. Nihon Geka Hokan. Archiv für Japanische Chirurgie 45(2): 123-133

Merrick, B.Alex.; Madenspacher, J.H., 2005: Complementary gene and protein expression studies and integrative approaches in toxicogenomics. Parallel transcript and protein profiling is a strategy to gain further insight into the mechanisms of toxicity and disease. The technologies used to measure expression at the transcript and protein levels each convey different information and hav...

Whitley, M., 1983: Administrative use of systems analysis to troubleshoot a problematic work situation. The deinstitutionalized mentally ill continue to seek housing. The present private nursing home industry has sought to fill this population's needs by expanding their business endeavors to low cost residential facilities. The industry, of cou...

Auclin, F.; Dufier, J.L.; Manderieux, N.; Sitruk, F.; Benoît, B.; Maroteaux, P.; Couly, G.; Aicardi, J.; Haye, C., 1989: Optic atrophy in children in Pyle's craniometaphyseal dysplasia. Bulletin des Societes D'ophtalmologie de France 89(8-9): 923-924

Wendrock-Shiga, G.; Mechtel, D.; Uhrlaß, S.; Koch, D.; Krüger, C.; Nenoff, P., 2017: Tinea barbae profunda due to Trichophyton mentagrophytes after journey to Thailand : Case report and review. Tinea barbae represents a very rare dermatophytosis. We report on a tinea barbae profunda following a journey to Southeast Asia. After travel to Thailand, a businessman was affected by a foudroyant proceeding abscessing infection of the upper...

Michaud V.; Clocchiatti R.; Sbrana S., 2000: The Minoan and post-Minoan eruptions, Santorini (Greece), in the light of melt inclusions; chlorine and sulphur behaviour. Mineralogical and melt inclusion studies were carried out on plinian tephra (Pumice fall unit, Phira) ejected during the first phase of the Minoan eruption, as well as on the most basic pyroclastic ejecta emitted during the recent activity of Nea...

Hale, R.W., 1987: Phasic approach to oral contraceptives. Oral contraceptives were initially very high in estrogen and progestogen and had a uniform dosage schedule throughout the entire cycle. These early oral contraceptives were associated with high rates of side effects and complications. As a result,...

Fanshawe, D.B., 1973: Vegetation of Chinsali district. Describes the vegetation, and lists the trees, shrubs and climbers found in the various forest and woodland types that occur in the district.

Romaire, R.; Merry, G., 2007: Effects of paddlewheel aeration on water quality in crawfish ponds. Journal of applied aquaculture 19(3): 61-75

O'neil M.B.; Hooper S.L.; Wagner R.J.; Marder E., 1985: Homologous pyloric muscles are innervated by different motor neurons that use different neurotransmitters in panulirus and cancer. Society for Neuroscience Abstracts 11(2): 1021