geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Delayed transplantation of olfactory ensheathing glia promotes sparing/regeneration of supraspinal axons in the contused adult rat spinal cord


, : Delayed transplantation of olfactory ensheathing glia promotes sparing/regeneration of supraspinal axons in the contused adult rat spinal cord. Journal of Neurotrauma 20(1): 1-16

The aim of this study was to determine the preferred time and environment for transplantation of olfactory ensheathing glia (OEG) into the moderately contused adult rat thoracic spinal cord. Purified OEG were suspended in culture medium with or without fibrinogen and injected into the contused cord segment at 30 min or 7 days after injury. Control animals received a contusion injury only or injection of only medium 7 days after contusion. The effects on axonal sparing/regeneration and functional recovery were evaluated 8 weeks after injury. The grafts largely filled the lesion site, reducing cavitation, and appeared continuous with the spinal nervous tissue. Whereas in 7d/medium only animals, 54% of spinal tissue within a 2.5-mm-long segment of cord centered at the injury site was spared, significantly more tissue was spared in 0 d/OEG-medium (73%), 0 d/OEG-fibrin (66%), 7 d/OEG-medium (70%), and 7 d/OEG-fibrin (68%) grafted animals. Compared with controls, the grafted animals exhibited more serotonergic axons within the transplant, the surrounding white matter, and the spinal cord up to at least 20 mm caudal to the graft. Retrograde tracing revealed that all but the 0 d/OEG-fibrin graft promoted sparing/regeneration of supraspinal axons compared with controls. Overall, the 7 d/OEG-medium group resulted in the best response, with twice as many labeled neurons in the brain compared with 7 d/medium only controls. Of the labeled neurons, 68% were located in the reticular formation, and 4% in the red, 4% in the raphe, and 5% in the vestibular nuclei. Hindlimb performance was modestly but significantly improved in the 7 d/OEG-medium group. Our results demonstrate that transplantation of OEG into the moderately contused adult rat thoracic spinal cord promotes sparing/regeneration of supraspinal axons and that 7 d transplantation is more effective than acute transplantation of OEG. Our results have relevant implications for future surgical repair strategies of the contused spinal cord.

US$19.90

PMID: 12614584

DOI: 10.1089/08977150360517146


Other references

Paul, A.; Acre, K.; Todd, K.J.; Wallace, D.; Todd, K.J.; Jernigan, A.; Wallig, M., 1992: Efficacy of ivermectin against Dirofilaria immitis in cats 30 and 45 days post-infection. Proceedings of the Heartworm Symposium '92: Austin Texas March 27-29-1992 119

Tsakris, A.; Papa, A.; Douboyas, J.; Antoniadis, A., 1997: Neonatal meningitis due to multi-resistant Listeria monocytogenes. Journal of Antimicrobial ChemoTherapy 39(4): 553-554

Sykora Y.; Charles A.W., 1989: Purification and characterization of phosphoenolpyruvate carboxylase from thiobacillus novellus atcc 8093. Abstracts of the Annual Meeting of the American Society for Microbiology 89: 269

Ledirac, N.; Delescluse, C.; Lesca, P.; Piechocki, M.P.; Hines, R.N.; de Sousa, G.; Pralavorio, M.; Rahmani, R., 2000: Diflubenzuron, a benzoyl-urea insecticide, is a potent inhibitor of TCDD-induced CYP1A1 expression in HepG2 cells. The effect of diflubenzuron (DFB) on cytochrome p450 1A1 (CYP1A1) expression and induction in human hepatoma cells (HepG2) was investigated. Treatment of HepG2 cells over 72 h with non-cytotoxic concentrations of DFB (0.5 to 25 micro M) strongly d...

Byers P.H.; Cohn D.H.; Wenstrup R.J.; Starman B.; Willing M.C.; Bonadio J.F., 1987: Secretion mutants of type i collagen. Journal of Cellular Biochemistry Supplement (11 PART A): 271

Templeman, C., 2004: Ovarian cysts. Journal of Pediatric and Adolescent Gynecology 17(4): 297-298

Hose, J.E.; Puffer, H.W., 1984: Oxygen consumption rates of grunion (Leuresthes tenuis) embryos exposed to the petroleum hydrocarbon, benzo[a]pyrene. Bioconcentration of [14C]benzo[a]pyrene and effects of unlabeled benzo[a]pyrene (BaP) accumulation on the routine oxygen consumption of embryonic grunion (Leuresthes tenuis) were studied. At Day 15, bioconcentration factors over dissolved BaP leve...

Raab-Traub, N.; Webster-Cyriaque, J., 1997: Epstein-Barr virus infection and expression in oral lesions. The prevalence of Epstein-Barr virus (EBV) and the recently discovered Kaposi's sarcoma associated herpes virus, human herpesvirus 8 (KSHV or HHV8), was determined within oral lesions common to HIV infection including OHL, pseudoOHL (PHL), or...

Del-Rio-Zaragoza, O.; Hernandez-Rodriguez, M.; Bueckle-Ramirez, L., F., 2008: Thermal stress effect on tilapia Oreochromis mossambicus Pisces Cichlidae blood parameters. The effect of thermal stress on blood parameters of Oreochromis mossambicus (Peters, 1852) was evaluated based on the critical thermal maximum (CTMax) and the upper incipient lethal temperature (UILT). The CTMax values in tilapia acclimated at 24...

Yang, C.P.; Guillan, R.A., 1982: Cardiovascular characteristics of Marfan's syndrome: inference for management. Southern Medical Journal 75(2): 230-231