geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Detection of endogenous agmatine content in rat brain and NG108-15 cells using high performance liquid chromatography


, : Detection of endogenous agmatine content in rat brain and NG108-15 cells using high performance liquid chromatography. Zhongguo Yaolixue Yu Dulixue Zazhi 16(6): 459-463, December

AIM: To develop a method to determine endogenous agmatine (Agm) content in rat brains and cultured NG108-15 cells. METHODS: High performance liquid chromatography (HPLC) with fluorescence detector was used to determine the endogenous Agm concentration in different regions (frontal cortex, thalamus, hippocampus, striatum and cerebellum) of rat brain and NG108-15 cells. RESULTS: The concentration of endogenous Agm was 0.8-1.71 mugcntdotg-1 wet tissues in different rat brain regions, and 0.044 mgcntdotg-1 protein in NG108-15 cells, respectively. CONCLUSION: This gradient HPLC procedure is applicable for routine detection of Agm in vertebrate tissues and cells.

US$29.90


Other references

Tierney, S., M.; Gonzales-Ojeda, T.; Wcislo, W., T., 2008: Nesting biology and social behavior of Xenochlora bees Hymenoptera Halictidae Augochlorini from Peru. The augochlorine (Halictidae) bee genus Xenochlora is the diurnal sister group to the nocturnal Megalopta, both of which are stem-nesting halictid bees. Here we present notes on the nesting biology and social behavior in four nests of Xenochlora...

Pratt, L.M.; Mai, B.Khanh.; Ramachandran, B.Ramu., 2012: Carbenoid alkene insertion reactions of oxiranyllithiums. The first computational investigations of the carbenoid reactions of α-lithiated dimethyl ether (methoxymethyllithium) and the intramolecular and intermolecular reactions of lithiated epoxides with the alkene double bond to yield cyclopropane rin...

Burke, G.; McCaughan, B.; Glanville, A., 2015: Metachronous tracheal squamous cell carcinoma treated with Nd: YAG laser. Tracheal squamous cell carcinoma detected early in a high-risk patient has been treated twice with local neodymium-yttrium aluminium garnet laser therapy with good result so far. Ongoing bronchoscopic monitoring continues.

Griff, S.; Ammenwerth, W.; Schönfeld, N.; Bauer, T.T.; Mairinger, T.; Blum, T-Gerriet.; Kollmeier, J.; Grüning, W., 2016: Erratum to: Morphometrical analysis of transbronchial cryobiopsies. Diagnostic Pathology 11(1): 64-64

Dumas B.R.; Brignon G.; Grosclaude F.; Mercier J C., 1971: The primary structure of bovine beta casein the alignment of 65 amino acid residues of the carboxyl terminus. European Journal of Biochemistry 20(2): 258-263

Katz, I., 1968: Automobile engines: pollution and power. Science 162(3859): 1221-1222

Vredevoe, D.; Kim, A.C.; Dambacher, B.; Call, J.D., 1969: Aggressive post-operative play responses of hospitalized preschool children. Nursing Research Report 4(2): 1 Passim-1 Passim

Hutchinson, J.B., 1952: The dissemination of cotton in Africa. Two types of Gossypium arboreum are established in Africa, race soudanense in the Sudan and W. Africa, and race indicum on the Tanganyika coast, and formerly in Zambesia. Of G. herbaceum, the annual typical form was the 1st cotton of the Nile Delt...

Olafson, K.M.; Ferraro, F.R., 2001: Effects of emotional state on lexical decision performance. The effect of emotional state on lexical processing was investigated. Subjects were randomly assigned to either a happy or sad mood condition. Emotional state was then induced by listening to 8 min of classical music previously rated to induce hap...

Barna, S.F.; Jacobs, K.E.; Mensing, G.A.; Ferreira, P.M., 2017: Electrochemical direct writing and erasing of silver nanostructures on phosphate glass using atomic force microscopy. This paper reports a liquid-free, mask-less electrochemical direct-write lithographic technique using an atomic force microscopy (AFM) probe for writing silver nanostructures in minutes on an optically transparent substrate. Under ambient conditio...