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Detection of endogenous agmatine content in rat brain and NG108-15 cells using high performance liquid chromatography

, : Detection of endogenous agmatine content in rat brain and NG108-15 cells using high performance liquid chromatography. Zhongguo Yaolixue Yu Dulixue Zazhi 16(6): 459-463, December

AIM: To develop a method to determine endogenous agmatine (Agm) content in rat brains and cultured NG108-15 cells. METHODS: High performance liquid chromatography (HPLC) with fluorescence detector was used to determine the endogenous Agm concentration in different regions (frontal cortex, thalamus, hippocampus, striatum and cerebellum) of rat brain and NG108-15 cells. RESULTS: The concentration of endogenous Agm was 0.8-1.71 mugcntdotg-1 wet tissues in different rat brain regions, and 0.044 mgcntdotg-1 protein in NG108-15 cells, respectively. CONCLUSION: This gradient HPLC procedure is applicable for routine detection of Agm in vertebrate tissues and cells.


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