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Determination of apparent binding constants for aroma compounds with beta-lactoglobulin by dynamic coupled column liquid chromatography

, : Determination of apparent binding constants for aroma compounds with beta-lactoglobulin by dynamic coupled column liquid chromatography. Journal of Agricultural and Food Chemistry 48(11): 5396-5400

Apparent binding constants of aroma compounds limonene, alpha- and beta-ionone, and terpenyl acetate, with beta-lactoglobulin (BLG), were determined, using dynamic coupled column liquid chromatography, for pH values varying from 3 to 11. K(a) values varied from 2.61 to 3.21 x 10(3) M(-1) for limonene, indicating a strong interaction with BLG. Similarly, significant and close apparent binding constants were obtained for alpha- and beta-ionone, 1.7 x 10(2) and 4.5 to 5.4 x 10(2) M(-1), respectively. These data indicated that a similar mechanism is involved for the binding of these two molecules. The weaker values obtained at low pH, for alpha-ionone relative to beta-ionone, can be explained by the existence of steric hindrance. An increase of the apparent binding constant was observed, for all the compounds studied, when the pH was increased from 3 to 9. At this pH, an apparent binding constant was obtained for terpenyl acetate (1.04 x 10(2) M(-1)), whereas this determination was not possible at pH 3 and 6. The apparent binding constant increase was in agreement with the decrease of aroma compound relative activity coefficient in the presence of BLG, previously observed at this pH. It indicated a best accessibility to the same binding site. The binding constants of all the aroma compounds studied decreased at pH 11 as a result of the important release of the BLG structure previously reported.


PMID: 11087491

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Van de Water, P.K.; Keever, T.; Main, C.E.; Levetin, E., 2003: An assessment of predictive forecasting of Juniperus ashei pollen movement in the Southern Great Plains, USA. Juniperus ashei pollen, a significant aeroallergen, has been recorded during December and January in Tulsa, Oklahoma, over the past 20 years. The nearest upwind source for this pollen is populations growing in southern Oklahoma and central Texas,...

Behrensmeyer Anna K., 1981: Vertebrate paleoecology in a Recent East African ecosystem. Pages 591-615 1981

Seress L., 1987: A golgi electron microscopic study of fusiform cells in the hilus of the hippocampal dentate gyrus. Anatomical Record: 113a-114a

Hu-Tsai, M.; Winter, J.; Woolf, C.J., 1992: Regional differences in the distribution of capsaicin-sensitive target-identified adult rat dorsal root ganglion neurons. A retrograde labelling technique combined with a cobalt uptake assay in cultured adult rat dorsal root ganglion (DRG) neurons were applied to study the distribution of capsaicin sensitivity in relation to different peripheral targets. This study s...

Malin, S.A.; Jeromin, A.; Nerbonne, J.M., 2001: Modulation of neuronal K+ currents by calcium sensors. This study was designed to investigate the role of Neuronal Ca++-Sensor (NCS) proteins in the regulation of neuronal excitability. In rat SCG neurons, NCS-1 is readily detected in association with plasma membranes. Previously, we have shown that S...

Grennan D.M.; Dyer P.A.; Clague R.; Dodds W.; Smeaton I.; Harris R., 1983: Family studies in rheumatoid arthritis the importance of hla dr 4 and of genes for auto immune thyroid disease. The segregation of HLA haplotypes with rheumatoid arthritis (RA) was studied in 13 selected, multicase families. HLA-DR4 was present in 77% of RA Probands (32% controls). No single haplotype was associated with RA in different families. Analysis o...

D.C.; Conklin J.L.; Christensen J., 1987: Origin of slow waves in the cat proximal colon. Gastroenterology 92(5 PART 2): 1376

Anderson, M.; Mendelsohn, J., 1972: A rapid salt-curing technique. Journal of food science: 37 (4) 627-628

Sarata, B.P.; Jeppesen, J.C., 1977: Job design and staff satisfaction in human service settings. It was hypothesized that job design is systematically related to employee satisfaction. The job design variables included were: variety, task-identity, feedback, autonomy participation, learning, and information. Subjects were 137 professional and...

Alleyne, G.A., 1968: Concentrations of metabolic intermediates in kidneys of rats with metabolic acidosis. Nature 217(5131): 847-848