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Determination of terbutaline enantiomers in human urine by capillary electrophoresis using hydroxypropyl-beta-cyclodextrin as a chiral selector


, : Determination of terbutaline enantiomers in human urine by capillary electrophoresis using hydroxypropyl-beta-cyclodextrin as a chiral selector. Archives of Pharmacal Research 26(2): 120-123

A method for the determination of terbutaline enantiomers in human urine by capillary electrophoresis has been developed. Optimum resolution was achieved using 50 mM phosphate buffer, pH 2.5, containing 15 mM of hydroxypropyl-beta-cyclodextrin as a chiral selector. Urine samples were prepared by solid-phase extraction with Sep-pak silica, followed by CE. The assay was linear between 2-250 ng/mL (R = 0.9998 for (S)-(+)-terbutaline and R = 0.9999 for (R)-(-)terbutaline) and detection limit was 0.8 ng/mL. The intra-day variation ranged between 6.3 and 14.5% in relation to the measured concentration and the inter-day variation was 8.2-20.1%. It has been applied to the determination of (S)-(+)terbutaline and (R)-(-)-terbutaline in urine from healthy volunteer dosed with racemic terbutaline sulfate.

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PMID: 12643586


Other references

Curfman, G.D.; Morrissey, S.; Drazen, J.M., 2009: Tobacco, public health, and the FDA. New England Journal of Medicine 361(4): 402-403

Cambridge, P., 1950: Hascot, a New Cragpit. Transactions of the Suffolk Naturalists' Society, 7(2): 66-67

Karavaev, V.M., 2011: Peculiarities of combined blunt head injuries in children and adolescents. The objective of the present study was to evaluate the frequency and peculiar features of combined head injuries in children and adolescents who suffered the fatal blunt trauma. A total of 101 corpses and 188 archive records were available for the...

Dotti A., 1970: Clinical trial on the anti tussive action of codeine phenyl toloxamine combination. Giornale Italiano delle Malattie del Torace 24(3): 147-157

Zapparoli, Marzio, 1996: Lithobius nuragicus n.sp., a new Lithobius from a Sardinian cave (Chilopoda Lithobiomorpha). A new species of Lithobius from a Sardinian cave (Cagliari, Santadi, loc. Su Benatzu, Grotta "Pirosu", 631 Sa/Ca, m 270) is described. Lithobius nuragicus n. sp. belongs to the subgenus Lithobius s. str. and is related to Lithobius varie...

Bolzoni, G.; Boni, P.; Magnino, S., 1991: Somatic cell count in milk: use of a freeze-dried standard in milk to verify opto-fluorometric instruments. Somatic cell count in milk is a very important indicator of mammary efficiency on dairy farms: it is used in all quality-payment schemes and is generally carried out with opto-fluorometric instruments. For this analysis, apart from direct microsco...

Remezova, E.S.; Aĭrapetova, E.S.; Andreeva, L.A.; Gorlina, I.S., 1984: Clinical picture and treatment of epilepsy and epileptic syndromes in children (according to the results of an epidemiologic study). On the basis of clinical-epidemiological studies, the authors present data on the prevalence of the convulsive syndrome in children and on the characteristics of major clinical manifestations in patients with epileptic attacks: the premorbid state...

Imperato, C.; Giovannelli, G.; Battistini, F.D.; Ghirardini, G., 1959: The metabolism of circulating plasma albumins in the dystrophic child, investigated with 1131-labeled albumin. La Pediatria 67: 549-592

Savoie, J.M.; Gourbiere, F., 1988: Enzyme patterns of the fungal species degrading abies alba mill. needles. The screening of enzymatic abilities in a fungal succession allowed to establish some bonds between the biochemical partitioning of the resources and the spatial- and temporal-structure of this fungal community.

Mihich, E.; Simpson, C.L.; Mulhern, A.I., 1965: Kethoxal bis(thiosemicarbazone). II. Toxic and pathologic effects. Cancer Research 25(9): 1417-1431