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Differential expression of sphingolipids in MRP1 overexpressing HT29 cells


, : Differential expression of sphingolipids in MRP1 overexpressing HT29 cells. International Journal of Cancer 87(2): 172-178, 15 July

We have obtained a novel multidrug resistant cell line, derived from HT29 G(+) human colon carcinoma cells, by selection with gradually increasing concentrations of the anti-mitotic, microtubule-disrupting agent colchicine. This HT29(col) cell line displayed a 25-fold increase in colchicine resistance and exhibited cross-resistance to doxorubicin, VP16, vincristine and taxol. Immunoblotting, combined with RT-PCR showed that the multidrug resistance phenotype was conferred by specific overexpression of the multidrug resistance protein 1. Confocal scanning laser microscopy revealed that multidrug resistance protein 1 specifically localized in the plasma membrane of HT29(col) cells. In a functional assay, using the fluorescent multidrug resistance protein 1 substrate 5-carboxyfluorescein, an increased efflux activity of HT29(col) cells was measured, as compared to the wild-type HT29 G(+) cells. MK571, a specific inhibitor of multidrug resistance protein 1, blocked the 5-carboxyfluorescein efflux, but only partially reversed resistance to colchicine, indicating that additional multidrug resistance mechanisms operate in HT29(col) cells. In conclusion, these results show for the first time overexpression of a functional multidrug resistance protein 1 under colchicine pressure, indicating that colchicine is not a P-glycoprotein-specific substrate. Colchicine-induced overexpression of multidrug resistance protein 1 is accompanied by a changed sphingolipid composition, i.e., enhanced levels of glucosylceramide and galactosylceramide. In addition, ceramide, a lipid messenger molecule involved in apoptosis-related signal transduction processes, was much more abundant in HT29(col) cells, which is indicative of a stress response.

US$19.90

PMID: 10861470

DOI: 10.1002/1097-0215(20000715)87:2<172::aid-ijc3>3.0.co;2-k


Other references

Rubtsov, M.I.; Yartiev, A.G., 1976: Genetic resources of fodder crops and their use in breeding. At the All-Union Institute of Fodder Crops, USSR, between 650 and 750 forms are studied annually, including 15 species of clover, 20 of lucerne, over 30 of fescue, 20 species each of brome grass and Agropyron, and also species of Melilotus, Lotus,...

Kadota, R.P.; Bowie, E.J.; Emslander, H.C.; Fass, D.N.; Ilstrup, D.M., 1987: The capillary thrombometer revisited. A modified capillary thrombometer was constructed to study the rate of thrombus formation using heparinized whole blood (2 U/ml) obtained from normal adults (n = 22) and children (n = 20) and patients with hemophilia A (n = 10) or von Willebrand&a...

Nolthenius, A.C.T., 1944: Canine teeth in Chital Axis axis stags. Journal of the Bombay Natural History Society, 451: 83-84

de Araújo-Jorge, T.C.; de Souza, W., 1986: Interaction of Trypanosoma cruzi with macrophages: effect of previous incubation of the parasites or the host cells with lectins. The effect of incubation with lectins of the macrophages or two evolutive stages of Trypanosoma cruzi (noninfective epimastigotes and infective trypomastigotes) on the ingestion of the parasites by mouse peritoneal macrophages was studied. Lectins...

Eufinger, H.; Wehmöller, M.; Machtens, E., 1998: Individual prostheses and resection templates for mandibular resection and reconstruction. This new technique uses helical computed tomography data and computer-aided design and manufacturing for preoperative fabrication of individual mandibular prostheses together with corresponding resection templates. Coherent 3D geometries for compu...

Barua, S.C., 1963: Growth rate of the tea leaf as determined by shade and nutrients. Six jat populations of tea[long dash]Camellia sinensis L. var. assamica (Masters) Kitamura[long dash]were studied. Growth rate of the leaf was defined as change in dry weight in 10 days, as the leaf position passed from the third ("initial&qu...

Khamidova M.Kh; Akhmedova F.A.; Gitin I.Ya; Kholmatov B.Kh, 1975: The soviet beta blocking agent anapriline in the treatment of ischemic heart disease patients. Meditsinskii Zhurnal Uzbekistana 11: 8-10

Kim, J.H.; Song, C.S., 1965: The L-ascorbic acid absorption. When about 300 mg of L-ascorbic acid was given intravenously to the adults saturated with the vitamin, the increased blood level returned to almost the original level in 3 hr. Also the amounts of the vitamin absorbed by the tissue cells and metabo...

Newcom, S.R.; Kadin, M.E., 1979: Prednisone in treatment of allergen-associated angio-immunoblastic lymphadenopathy. Angio-immunoblastic lymphadenopathy with dysproteinaemia (A.I.L.D.) is a lymphoproliferative disorder associated in one third of the patients with exposure to an allergen. In four patients with allergen-associated A.I.L.D. single cytotoxic drugs w...

Akimova, G.P.; Rodchenko, O.P., 1977: Lignification of root cells of maize with reduced growth at low temperature. During the cell elongation stage, lignification of root cell walls was greater in maize grown at 10 deg C than in that grown at 27 deg (control). It was suggested that cell walls are lignified before they reach their full size and that lignificati...