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Disabled-2 small interfering RNA modulates cellular adhesive function and MAPK activity during megakaryocytic differentiation of K562 cells


, : Disabled-2 small interfering RNA modulates cellular adhesive function and MAPK activity during megakaryocytic differentiation of K562 cells. FEBS Letters 541(1-3): 21-27, 24 April

Previous studies have shown that Disabled-2 (DAB2) is up-regulated during megakaryocytic differentiation of human K562 cells. To delineate the consequences of DAB2 induction, a DNA vector-based small interfering RNA (siRNA) was designed to intervene in DAB2 expression. We found that DAB2 siRNA specifically inhibited DAB2 induction, resulting in the modulation of cell-cell adhesion and mitogen-activated protein kinase (MAPK) phosphorylation. The morphological changes and beta3 integrin expression associated with megakaryocytic differentiation were not affected. Since the MAPK pathway has been shown to involve DAB2 induction [Tseng et al., Biochem. Biophys. Res. Commun. 285 (2001) 129-135], our results suggest a reciprocal regulation between DAB2 and MAPK in the differentiation of K562 cells. In addition, we have demonstrated for the first time that DAB2 siRNA is a valuable tool for unveiling the biological consequences of DAB2 expression.

US$19.90

PMID: 12706813

DOI: 10.1016/s0014-5793(03)00281-3


Other references

Krenzelok, E.P.; Jacobsen, T.D.; Aronis, J.M., 1995: Botanical scoundrels and emergency department visits. Journal of Toxicology Clinical Toxicology 33(5): 543-544

Zakrzewski S.F., 1970: Metabolism of folic acid in a mutant strain 8081 s of pediococcus cerevisiae 8081. Federation Proceedings 29(2): 943

Kaczmarek, M.M.; Krawczynski, K.; Blitek, A.; Kiewisz, J.; Schams, D.; Ziecik, A.J., 2011: Seminal plasma affects prostaglandin synthesis in the porcine oviduct. Seminal fluids introduced to the female reproductive tract at mating can affect subsequent events, such as ovulation, fertilization, conception, and pregnancy. Bioactive molecules present in seminal plasma can modify the cellular composition, stru...

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Revelante, N.; Gilmartin, M., 1978: Characteristics of the micro plankton and nano plankton communities of an australian coastal plain estuary. The relative importance of micro- and nanoplankton was evaluated in the Parramatta Estuary and Sydney Harbor, a culturally eutrophicated southern hemisphere estuarial complex, during a non-upwelling period following an extended drought. Nanoplankt...

Balech, E., 1978: Estructura de Amphisolenia bidentata Schroder (Dinoflagellata). Physis Seccion A los Oceanos y sus Organismos, 3793: 25-32

Lyon, M.E.; Lyon, A.W., 2011: Analysis of the performance of the CONTOUR® TS Blood Glucose Monitoring System: when regulatory performance criteria are met, should we have confidence to use a medical device with all patients?. The article entitled, Performance of the CONTOUR® TS Blood Glucose Monitoring System, by Frank and colleagues in this issue of Journal of Diabetes Science and Technology, demonstrates that the CONTOUR® TS glucose meter exceeds current regulatory...

Zhou, T.S.; Zhou, R.H., 1993: Macrological identification of original and commercial Chinese drug gusuibu (Rhizoma drynariae). Twelve original species and 7 commercial species of Chinese drug Gusuibu were identified and discussed based on macrological observation and comparison. The survey of 65 samples collected from 23 provinces throughout the country showed that at the...

Gruden, K.; Kuipers, A.G.J.; Guncar, G.; Slapar, N.; Strukelj, B.; Jongsma, M.A., 2004: Molecular basis of Colorado potato beetle adaptation to potato plant defence at the level of digestive cysteine proteinases. Potato synthesizes high levels of proteinase inhibitors in response to insect attack. This can adversely affect protein digestion in the insects, leading to reduced growth, delayed development and lowered fecundity. Colorado potato beetle overcome...