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Disabled-2 small interfering RNA modulates cellular adhesive function and MAPK activity during megakaryocytic differentiation of K562 cells

, : Disabled-2 small interfering RNA modulates cellular adhesive function and MAPK activity during megakaryocytic differentiation of K562 cells. FEBS Letters 541(1-3): 21-27, 24 April

Previous studies have shown that Disabled-2 (DAB2) is up-regulated during megakaryocytic differentiation of human K562 cells. To delineate the consequences of DAB2 induction, a DNA vector-based small interfering RNA (siRNA) was designed to intervene in DAB2 expression. We found that DAB2 siRNA specifically inhibited DAB2 induction, resulting in the modulation of cell-cell adhesion and mitogen-activated protein kinase (MAPK) phosphorylation. The morphological changes and beta3 integrin expression associated with megakaryocytic differentiation were not affected. Since the MAPK pathway has been shown to involve DAB2 induction [Tseng et al., Biochem. Biophys. Res. Commun. 285 (2001) 129-135], our results suggest a reciprocal regulation between DAB2 and MAPK in the differentiation of K562 cells. In addition, we have demonstrated for the first time that DAB2 siRNA is a valuable tool for unveiling the biological consequences of DAB2 expression.


PMID: 12706813

DOI: 10.1016/s0014-5793(03)00281-3

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Krenzelok, E.P.; Jacobsen, T.D.; Aronis, J.M., 1995: Botanical scoundrels and emergency department visits. Journal of Toxicology Clinical Toxicology 33(5): 543-544

Zakrzewski S.F., 1970: Metabolism of folic acid in a mutant strain 8081 s of pediococcus cerevisiae 8081. Federation Proceedings 29(2): 943

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