+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Diurnal variation in the renal excretion of modified RNA catabolites in humans

, : Diurnal variation in the renal excretion of modified RNA catabolites in humans. Clinical Science 105(2): 195-202

Renally excreted modified RNA catabolites [pseudouridine (psi), N (2), N (2)-dimethylguanosine (m(2)(2)G) and N (6)-threoninocarbonyladenosine (t(6)A)] are markers of whole-body rates of degradation of rRNA and tRNA, and are thought to be potential indicators of the resting metabolic rate. To investigate diurnal variations of these RNA catabolites, the amounts of psi, m(2)(2)G and t(6)A excreted were determined by HPLC of the urine from eight healthy male adults collected over 47-h periods, which were subdivided into the morning (06.00 or 09.00 to 12.00 hours), the afternoon (12.00 to 18.00 hours), the evening (18.00 to 24.00 hours) and the night (00.00 to 06.00 or 08.00 hours), under two different nutritional regimens with 100 or 50 g of protein/day. Furthermore, rates of degradation of rRNA and tRNA were calculated using values for these RNA catabolites. For comparison, the corresponding excretion of creatinine, which originates from the energy metabolism of muscle, and of 3-methylhistidine (m(3)His), which is an indicator of muscle protein degradation, was determined. Differences in excretion during the collection periods were tested using the Friedmann test. The excretion of psi, creatinine and m(3)His (micromol x h(-1) x kg(-1)) altered significantly (P <0.001) during the day. Medians were: for psi, 0.21 (morning), 0.19 (afternoon), 0.19 (evening) and 0.18 (night); for creatinine, 8.8, 8.4, 8.0 and 7.3 respectively; for m(3)His, 0.13, 0.11, 0.12 and 0.10 respectively. The excretion rates of m(2)(2)G and t(6)A (nmol x h(-1) x kg(-1)) altered, but not significantly, during the day; corresponding medians were: for m(2)(2)G, 9.0, 8.4, 8.0 and 8.4 respectively; for t(6)A, 4.3, 4.1, 3.9 and 3.9 respectively. From these results it can be concluded that, in order to assess the average daily rates of degradation of tRNA and rRNA using modified RNA catabolites, urine collection should be carried out quantitatively over 24 h periods. Likewise, the catabolites creatinine and m(3)His must be determined using 24 h urine samples when average daily excretion values are required.


PMID: 12708967

DOI: 10.1042/CS20020253

Other references

Debowski, P., 2004: Fish assemblages in the Slupia River system (northern Poland). Electrofishing was carried out at 66 sites in the Slupia River system. Twenty-five fish species were noted. The most frequent were trout (Salmo trutta), three-spined stickleback (Gasterosteus aculeatus) and bullhead (Cottus gobio). Three groups of...

Lochner, W.; Mercker, H.; Nasseri, M., 1959: On the anaerobic energy production of the homotherm heart in situ in cyanide poisoning. Naunyn-Schmiedebergs Archiv für Experimentelle Pathologie und Pharmakologie 236: 365-381

Bove, E.L.; Ohye, R.G.; Devaney, E.J.; Kurosawa, H.; Shin'oka, T.; Ikeda, A.; Nakanishi, T., 2007: Anatomic correction of congenitally corrected transposition and its close cousins. Cardiology in the Young 16 Suppl 3: 85-90

Gahl, W.A.; Dalakas, M.C.; Charnas, L.; Chen, K.T.; Pezeshkpour, G.H.; Kuwabara, T.; Davis, S.L.; Chesney, R.W.; Fink, J.; Hutchison, H.T., 1988: Myopathy and cystine storage in muscles in a patient with nephropathic cystinosis. We describe a case of recurrent hypoglycemia apparently caused by secretion of insulin-like growth factor II (IGF-II) by a leiomyosarcoma. A 67-year-old woman presented with recurrent severe hypoglycemia and a large mass in the thorax. During hypo...

Jarvis, A.; Guarino, L.; Williams, D.; Williams, K.; Vargas, I.; Hyman, G., 2002: Spatial analysis of wild peanut distributions and the implications for plant genetic resources conservation. Domesticated crop plants and their related wild species represent the biological foundation upon which world food security is based, yet these important components of biological diversity are imminently threatened by genetic erosion in situ. Spati...

Cope Louis W.; Jonson David C. (compiler), 2004: Greenland; a potential trove of minerals. Mining Engineering 56(10): 12-18

Napier J.A.L., 1991: Energy changes in a rockmass containing multiple discontinuities. Journal of the South African Institute of Mining and Metallurgy 91(5): 145-157

Brown, T.; Galea, V.; Mccomas, A., 1997: Loss of twitch torque following muscle compression. With the elbow flexed, compression of the human biceps brachii has been found to reduce twitch torque, with an approximately linear relationship being observed between the loss of torque and the applied pressure (up to 45 kPa). The decline in torq...

Asano, K.; Wu, Z.; Srinontong, P.; Ikeda, T.; Nagano, I.; Morita, H.; Maekawa, Y., 2016: Nonencapsulated Trichinella pseudospiralis Infection Impairs Follicular Helper T Cell Differentiation with Subclass-Selective Decreases in Antibody Responses. Infection and Immunity 84(12): 3550-3556

Goto, H.; Inaba, M., 2005: Ibandronate as a new therapeutic agent for osteoporosis. Ibandronate, a second generation amino-bisphosphonate has a high potency as much as the third generation bisphosphonates. Therefore this compound can be characteristically administrated by means of bolus intravenous injection. Moreover it is the f...