+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Ectodermal Wnt3/beta-catenin signaling is required for the establishment and maintenance of the apical ectodermal ridge

, : Ectodermal Wnt3/beta-catenin signaling is required for the establishment and maintenance of the apical ectodermal ridge. Genes & Development 17(3): 394-409

The formation of the apical ectodermal ridge (AER) is critical for the distal outgrowth and patterning of the vertebrate limb. Recent work in the chick has demonstrated that interplay between the Wnt and Fgf signaling pathways is essential in the limb mesenchyme and ectoderm in the establishment and perhaps the maintenance of the AER. In the mouse, whereas a role for Fgfs for AER establishment and function has been clearly demonstrated, the role of Wnt/beta-catenin signaling, although known to be important, is obscure. In this study, we demonstrate that Wnt3, which is expressed ubiquitously throughout the limb ectoderm, is essential for normal limb development and plays a critical role in the establishment of the AER. We also show that the conditional removal of beta-catenin in the ventral ectodermal cells is sufficient to elicit the mutant limb phenotype. In addition, removing beta-catenin after the induction of the ridge results in the disappearance of the AER, demonstrating the requirement for continued beta-catenin signaling for the maintenance of this structure. Finally, we demonstrate that Wnt/beta-catenin signaling lies upstream of the Bmp signaling pathway in establishment of the AER and regulation of the dorsoventral polarity of the limb.


PMID: 12569130

DOI: 10.1101/gad.1044903

Other references

Rosenberg D.J., 1987: Cardiotomy reservoir us patent 4662906 may 5 1987. Official Gazette Of The United States Patent & Trademark Office Patents1: 332-333

Treacy, R.; Yates, J.R.W.; Whittaker, J.L., 2003: Tuberous sclerosis mutation analysis Evaluation of a clinical questionnaire. Journal of Medical Genetics 40(Supplement 1): S37, September

Becker, H.; McDougall, G.J.; Douglas, N.E.; Arheart, K.L., 2008: Comparing the efficiency of eight-session versus four-session memory intervention for older adults. Evidence that reduced treatment achieves similar outcomes is beneficial because shorter interventions may be more cost-effective and more acceptable to participants. We examined the effects of shortening a memory intervention for elders from eight...

Schütz, M.; Haas, N.P., 2002: LISS--internal plate fixator. The LISS DF (less invasive stabilisation system) is a new internal fixator for the operative treatment of distal femur fractures following the principles of minimally invasive surgery. The fixation of the implant is based on screws providing angul...

Stark D., 1976: Lease of federal geothermal energy; rocky road. AAPG Bulletin 60(8, New concepts of exploration in Rockies(AAPG-SEPM meeting): 1410

Bossola, M.; Sanguinetti, M.; Scribano, D.; Zuppi, C.; Giungi, S.; Luciani, G.; Torelli, R.; Posteraro, B.; Fadda, G.; Tazza, L., 2009: Circulating bacterial-derived DNA fragments and markers of inflammation in chronic hemodialysis patients. Bacterial-derived DNA fragments (BDNAs) have been shown to be present in dialysis fluid, to pass through dialyzer membranes, and to induce IL-6 (IL-6) in mononuclear cells. The present study aimed at assessing the eventual presence of BDNAs in the...

Wolf, S., 1991: Suicidal crisis. Zeitschrift für Arztliche Fortbildung 85(10): 465-6, 468

Nelson, J.; Valentino, L.; Iacono, L.; Ropollo, P.; Cineas, N.; Stuart, S., 2016: Measuring workload of nurses on a neurosurgical care unit. The aim of this study was to create a model of workload that could be used to manage workload and increase satisfaction of workload for nurses on a neuroscience care unit. No study was found that delineated a model of workload that could be used t...

Pick, U.; Weiss, M., 1991: Polyphosphate Hydrolysis within Acidic Vacuoles in Response to Amine-Induced Alkaline Stress in the Halotolerant Alga Dunaliella salina. The location and mobilization of polyphosphates in response to an amine-induced alkaline stress were studied in the halotolerant alga Dunaliella salina. The following observations suggest that polyphosphates accumulate in acidic vacuoles: (a) Accu...

Laguens, G.; D.G.rolamo, V.; Spinelli, O.; Laguens, R.P., 1996: Identification of a tumor antigen associated to the estrogen receptor in axillary lymph nodes of breast cancer patients. Sixteen axillary lymph nodes were incubated with sera from patients with mammary carcinoma. Using immunofluorescence staining sera recognized antigenic determinants on follicular dendritic cells (FDC) within the follicle centers. These results wer...