+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Ectopic expression of negative ARNT2 factor disrupts fish development

, : Ectopic expression of negative ARNT2 factor disrupts fish development. Biochemical and Biophysical Research Communications 282(2): 487-492

ARNT factors are a cluster of bHLH-PAS factors that heterodimerize with other specific bHLH-PAS factors to mediate a wide range of biological responses. Previously, we obtained a truncated form of ARNT2-like factor, ARNT2A, from zebrafish, which encompasses the basic-helix-loop-helix and PAS A/B domains, but lacks a transactivation domain at its carboxyl end. Herein, we report another truncated ARNT2-like factor, ARNT2X, in zebrafish, which differs from ARNT2A at its N-terminal region. In cultured ZLE cells, transiently expressed ARNT2X and ARNT2A inhibited 2,3,7,8-TCDD-activated cyp1a1 transcription with different efficiencies. In the developing embryo, arnt2X mRNA was consistently expressed in the retinal and neural tube regions until the hatching stages, but it exhibited a more specific pattern at larval stages, including expression in the brain, eyes, hypothalamus, pharyngeal skeleton, heart, liver, pronephros duct, pectoral fin, and epithelial cells of the swim bladder. In contrast, arnt2A transcription diminished after hatching. Microinjecting a recombinant arnt2X-expression vector into fertilized eggs before cleavage stages caused severe defects in brain, eyes, pectoral fin, heart, and gut development. This suggests that the ARNT-mediated signal transduction pathways play important roles in fish tissue development.


PMID: 11401485

DOI: 10.1006/bbrc.2001.4525

Other references

Lian, C., 1965: Exclusively maximal arterial hypertension of atherosclerotic origin must not be treated with hypotensive drugs. Actualites Cardiologiques et Angeiologiques Internationales 14(2): 73-74

Gerber, S.; Rozet, J.-Michel; Takezawa, S.-Ichiro; Dos-Santos, L.-Coutinho; Lopes, L.; Gribouval, O.; Penet, C.; Perrault, I.; Ducroq, D.; Souied, E.; Jeanpierre, M.; Romana, S.; Frezal, J.; Ferraz, F.; Yu-Umesono, R.; Munnich, A.; Kaplan, J., 2000: The photoreceptor cell-specific nuclear receptor gene (PNR) accounts for retinitis pigmentosa in the Crypto-Jews from Portugal (Marranos), survivors from the Spanish Inquisition. The last Crypto-Jews (Marranos) are the survivors of Spanish Jews who were persecuted in the late fifteenth century, escaped to Portugal and were forced to convert to save their lives. Isolated groups still exist in mountainous areas such as Belmo...

Cecere, R.; Bertini, C.; Maier, M.E.; Làdavas, E., 2015: Unseen fearful faces influence face encoding: evidence from ERPs in hemianopic patients. Visual threat-related signals are not only processed via a cortical geniculo-striatal pathway to the amygdala but also via a subcortical colliculo-pulvinar-amygdala pathway, which presumably mediates implicit processing of fearful stimuli. Indeed,...

Neufeld, D.S.; Wright, S.H., 1994: Basolateral taurine transport in isolated, perfused Mytilus gill. FASEB Journal 8(4-5): A70

Rummukainen, U., 1971: Control of snow blight due to P. infestans by dusting with quintozene. In Finland the heaviest losses of Scots pine (Pinus sylvestris) in the nursery and in plantations are caused by P. infestans, 90-100% of the trees being killed in the worst cases. Dusting (20% a.i., 20-50 kg/ha) reduced incidence from 20.9 to 2.1%...

Provorov N.A., 1991: Origin and evolution of legume rhizobial symbiosis. Different ways of origin of root nodule bacteria have been analyzed. Evolution of legume-rhizobial symbiosis is a result of coevolution of the nodule bacteria and host plants. Host plant plays the leading part in coevolution. Coevolution mechanism...

Takov, R.; Boshnakova, D.; Viiachki, I.; Sedloev, S.; Khristov, I., 1977: Description of a case of esophageal achalasia. Khirurgiia 30(5): 432-435

Zardini, V., 1966: Obscure giant-cell syndrome with adeno-hepato-splenomegaly and general involvement in an infant, with restitutio ad integrum. Minerva Pediatrica 18(31): 1884-1890

Orr Tade; Osterkamp W.R., 1999: Vigil network data now online. Eos, Transactions, American Geophysical Union 80(31): 346

SHAPIRO.M.Ia.; DZHAGANOV.I.K., 1953: Formalin therapy following radical surgery of the middle ear. Vestnik Otorinolaringologii 15(6): 80-80