+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Ectopic expression of protein kinase CbetaII, -delta, and -epsilon, but not -betaI or -zeta, provide for insulin stimulation of glucose uptake in NIH-3T3 cells

, : Ectopic expression of protein kinase CbetaII, -delta, and -epsilon, but not -betaI or -zeta, provide for insulin stimulation of glucose uptake in NIH-3T3 cells. Archives of Biochemistry and Biophysics 372(1): 69-79

Insulin regulates a diverse array of signaling pathways involved in the control of growth, differentiation, proliferation, and metabolism. Insulin increases in glucose uptake via a protein kinase C-dependent pathway in target tissues such as fat and muscle are well documented. Insulin-regulated events, however, occur in all cells. The utilization of glucose as a preferred energy source is a ubiquitous event in eukaryotic cells. In NIH-3T3 fibroblasts, insulin treatment increased levels of the cPKC and nPKC activator, diacylglycerol. Insulin-responsive 2-[(3)H]deoxyglucose uptake was stimulated in a dose-dependent manner. The overexpression of protein kinase C (PKC)betaI, -betaII, -delta, -epsilon, and -zeta was used to investigate the specificity of PKC isozymes for insulin-sensitive glucose uptake. The stable overexpression of PKCbetaII, -delta, and -epsilon resulted in increases in insulin-stimulated 2-[(3)H]deoxyglucose uptake compared to vector control cells, while basal 2-deoxyglucose uptake levels were not elevated. Overexpression of PKCbetaI and PKCzeta isozymes had no further effect on basal or insulin-stimulated 2-deoxyglucose uptake. The PKC-specific inhibitor, CGP41251, blocked insulin effects on 2-deoxyglucose uptake but not its effects on tyrosine phosphorylation of cellular substrates. Insulin-stimulated 3-O-methylglucose uptake was also greater in cells overexpressing PKCbetaII, -delta, and -epsilon, compared to control cells. The increased responsiveness was not accompanied by conversion of 3T3 cells to the adipocyte phenotype or the increased expression of insulin receptors or glucose transporters (GLUT1-type). Insulin-stimulated recruitment of GLUT1 to plasma membranes of cells overexpressing PKCbetaII, -delta, and -epsilon, was greater than that in control cells. The data suggest that more than one PKC isozyme is involved in insulin signaling pathways in fibroblasts, resulting in increased GLUT1 transporter recruitment to cell membranes.


PMID: 10562418

DOI: 10.1006/abbi.1999.1472

Other references

Nizankowska, E.; Soja, J.; Pinis, G.; Bochenek, G.; Sładek, K.; Domagała, B.; Pajak, A.; Szczeklik, A., 1995: Treatment of steroid-dependent bronchial asthma with cyclosporin. The treatment of chronic severe asthma is unsatisfactory for many patients. The aim of the study was to determine the effects of treatment of steroid-dependent asthma with cyclosporin. We performed a double-blind, placebo-controlled, randomized, p...

Orimo, H.; Ito, H., 1976: Relationship between diabetes mellitus and impairment of carbohydrate metabolism of the aged. Nihon Ronen Igakkai Zasshi. Japanese Journal of Geriatrics 13(3): 157-162

Anonymous, 1960: Geology, Wolf Lake, Yukon Territory. Preliminary series geological map of about 4,300 sq. mi. just E. of Teslin and bordering the Yukon/British Columbia boundary. All Paleozoic systems are probably represented, but extensive metamorphism limits accurate system dating, although there...

Kuster, T.M.; Schleppi, P.; Hu, B.; Schulin, R.; Günthardt-Goerg, M.S., 2013: Nitrogen dynamics in oak model ecosystems subjected to air warming and drought on two different soils. Being tolerant to heat and drought, oaks are promising candidates for future forestry in view of climate change in Central Europe. Air warming is expected to increase, and drought decrease soil N availability and thus N supply to trees. Here, we c...

Avilion, A.A.; Bell, D.M.; Lovell-Badge, R., 2000: Micro-capillary tube in situ hybridisation: a novel method for processing small individual samples. We have developed a strategy to individually analyse large numbers of small tissue samples by RNA in situ hybridisation. Samples of approximately 0.4 mm X 0.5 mm are processed in rectangular capillary tubes fitted with nylon mesh and glass beads u...

Ji-Xian, G.; Handa, S.S.; Pezzuto, J.M.; Kinghorn, A.D.; Farnsworth, N.R., 1984: Plant Anticancer Agents XXXIII. Constituents of Passerina vulgaris1. Two lignans of known structure, (+)-syringaresinol, a cytotoxic agent, and (+)-nortrachelogenin, a compound with demonstrated antileukemic activity, were isolated from a biologically active extract of the stems of PASSERINA VULGARIS.

Alexander Van Geen; Edward A.B.yle; Willard S.M.ore, 1991: Trace metal enrichments in waters of the Gulf of Cadiz, Spain. Dissolved Cu, Cd, and Zn concentrations in continental shelf waters of the Gulf of Cadiz are much higher than reported for other coastal areas. Direct observations of the metal enrichments in this region were made in March–April 1986 and October...

Heise, U., 1971: Eine Wochenstube der Kleinen Hufeisennase, Rhinolophus hipposideros, and weitere Nachweise aus dem Eichsfeld. Hercynia: 307-311

Xu, L.; Deng, X., 2004: Tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone induces phosphorylation of mu- and m-calpain in association with increased secretion, cell migration, and invasion. Mounting evidence indicates that cigarette smoking not only promotes tumorigenesis but also may increase the spread of cancer cells in the body. However, the intracellular mechanism(s) by which cigarette smoking promotes metastasis of human lung c...

Zhang, Y.; Collier, F.; Naselli, G.; Saffery, R.; Tang, M.L.K.; Allen, K.J.; Ponsonby, A-Louise.; Harrison, L.C.; Vuillermin, P.; Molloy, J.; Burgner, D.; Ranganathan, S.; Dwyer, T.; O'Hely, M.; Richie, M., 2016: Cord blood monocyte-derived inflammatory cytokines suppress IL-2 and induce nonclassic "T(H)2-type" immunity associated with development of food allergy. Food allergy is a major health burden in early childhood. Infants who develop food allergy display a proinflammatory immune profile in cord blood, but how this is related to interleukin-4 (IL-4)/T helper 2 (T(H)2)-type immunity characteristic of a...