+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Editing and diagonal peak suppression in three-dimensional HCCH protein NMR correlation experiments

, : Editing and diagonal peak suppression in three-dimensional HCCH protein NMR correlation experiments. Journal of Biomolecular NMR 19(1): 69-73, January

A novel three-dimensional (3D) HCCH NMR experiment is introduced. It involves 13C-13C COSY or TOCSY coherence transfer plus two independent editing steps according to the number of protons attached to the individual carbons before and after the 13C-13C homonuclear mixing. This double editing leads to simplification of HCCH protein side chain spectra that otherwise are prone to spectral overlap. Another interesting feature is amino acid selectivity, i.e. that the presence of certain correlations in a doubly edited HCCH subspectrum gives a clue as to assignment to a particular subgroup of amino acids or segments thereof. Finally, the selection of two different multiplicities in the two editing steps leads to diagonal peak suppression in the 1H-1H (3D spectrum recorded with two 1H and one 13C dimension) or the 13C-13C (3D spectrum recorded with one 1H and two 13C dimensions) two-dimensional projection. The new experiment is demonstrated using a 13C,15N-labeled protein sample, chymotrypsin inhibitor 2, at 500 MHz.


PMID: 11246856

DOI: 10.1023/a:1008359912748

Other references

Anonymous, 2009: Winningham and preusser's critical thinking cases in nursing Barbara A Preusser Winningham and preusser's critical thinking cases in nursing Fourth Elsevier 720pp £24.99 978 0 323 05359 4 0323053599 [Formula: see text. NlmCategory="UNASSIGNED">At 720pages, this is a weighty book, offering 148 case studies in a range of clinical specialties. And at £24.99, it may appear excellent value for money, especially for impoverished students.

Fert Soc Agric Group (Engl), 1977: Symposium of the fertilizer society and agriculture group on phosphorus london england february 15 1977. Journal Of The Science Of Food & Agriculture: 654-659

Pasnik, Grzegorz, 2001: Six new species of Aleocharinae from Australia (Coleoptera: Staphylinidae). Six new species of Aleocharinae from Australia are described and illustrated: Placusa (s. str.) fauveli sp. n., P. (s. str.) tasmanica sp. n., P. (s. str.) australiensis sp. n., P. (Calpusa) quadripunctata sp. n., Leucocraspedum longisetosum sp. n...

Skriabin, V.V., 1966: Method for amplitude-frequency analysis of rheoencephalograms. Biulleten' Eksperimental'noi Biologii i Meditsiny 62(8): 123-125

Charlet J.M.; Dupuis C.; Quinif Y.; Bouko P.; Lair P., 1982: Natural thermoluminescence of quartz and feldspars, application of a new method in uranium exploration. Pages 545-556 1982

Robbie, Ta, 1971: Queen Elizabeth Forest Park. Winter 72, 51 (1) 231-237

Näslund, I.; Ottosson, J., 2016: A better follow-up of obesity surgery needed. Lakartidningen 112: -

Kita, M.; Ito, K., 2013: The caregiving process of the family unit caring for a frail older family member at home: a grounded theory study. To explore the caregiving process of family units caring for a frail older family member at home. Previous research on family caregiving has demonstrated the interdependence between individual members' health and family status and suggested t...

Gärtner, H.V.; Wehner, H.; Bohle, A., 1978: The immunohistological findings in various forms of glomerulonephritis: a comparative investigation based on 335 renal biopsies. Part I: Endocapillary glomerulonephritis, mesangioproliferative glomerulonephritis, mesangioproliferative glomerulonephritis with focal crescents, minimal proliferating intercapillary glomerulonephritis (without nephrotic syndrome). Pathology, Research and Practice 162(2): 178-197

Schwab, M., 2009: Intrauterine programming of disorders of brain function in later life. Fetal malnutrition or prenatally enhanced stress hormone levels have the potential to program brain function in later life. Even modest malnutrition has direct effects on brain development mainly via modulation of the insulin-like growth factor sy...