geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Editing site recognition and nucleotide insertion are separable processes in Physarum mitochondria


, : Editing site recognition and nucleotide insertion are separable processes in Physarum mitochondria. EMBO (European Molecular Biology Organization) Journal 21(22): 6154-6161, November 15

Insertional RNA editing in Physarum polycephalum is a complex process involving the specific addition of non-templated nucleotides to nascent mitochondrial transcripts. Since all four ribonucleotides are substrates for the editing activity(s), both the site of insertion and the identity of the nucleotide to be added at a particular position must be specified, but the signals for these events have yet to be elucidated. Here we report the occurrence of sporadic errors in RNAs synthesized in vitro. These mistakes, which include omission of encoded nucleotides as well as misinsertions, occur only on templates that support editing. The pattern of these misediting events indicates that editing site recognition and nucleotide addition are separable events, and that the recognition step involves features of the mitochondrial template that are required for editing. The larger deletions lack all templated nucleotides between editing sites, suggesting that the transcription/editing apparatus can "jump" from one insertion site to another, perhaps mediated by interactions with editing determinants, while smaller omissions most likely reflect misalignment of the transcript upon resumption of templated RNA synthesis.

US$19.90

PMID: 12426387

DOI: 10.1093/emboj/cdf610


Other references

Mink, J.H.; Richardson, A.; Grant, T.T., 1979: Evaluation of glenoid labrum by double-contrast shoulder arthrography. Double-contrast shoulder arthrography was used to define the normal and abnormal arthrographic anatomy of the glenoid labrum. A retrospective study of 12 patients suspected of having labral abnormalities or who had unexplained shoulder disability...

Placzek, M.; Dale, K., 1999: Tissue recombinations in collagen gels. Methods in Molecular Biology 97: 293-304

Tokuz R.Y., 1987: Response of phthalate esters acclimated activated sludge to 2 4 dinitrophenol. Water Science & Technology 19(3-4): 461-470

Fried, L.C., 1973: Atlanto-axial fracture-dislocations. Failure of posterior C.1 to C.2 fusion. Journal of Bone and Joint Surgery. British Volume 55(3): 490-496

Giacoma, T.; Ingersoll, G.L.; Williams, M., 1999: Teaching video effect on renal transplant patient outcomes. A study was undertaken to test the effect of a teaching video on renal transplant recipient outcomes. A quasi-experimental, pretest/posttest design was used. Fifty-nine adults undergoing renal transplantation were studied. Subjects were randomly a...

Camero, M.; Marinaro, M.; Losurdo, M.; Larocca, V.; Bodnar, L.; Patruno, G.; Buonavoglia, C.; Tempesta, M., 2016: Caprine herpesvirus 1 (CpHV-1) vaginal infection of goats: clinical efficacy of fig latex. The latex of Ficus carica Linn. (Moraceae) has been shown to interfere with the replication of caprine herpesvirus (CpHV)-1 in vitro. The present study was undertaken to determine the efficacy of vaginal administration of fig latex in goats experi...

Danel, I.; Graham, W.; Stupp, P.; Castillo, P., 1996: Applying the sisterhood method for estimating maternal mortality to a health facility-based sample: a comparison with results from a household-based sample. Background: The sisterhood method is an indirect technique used to estimate maternal mortality in developing countries, where maternal deaths are often poorly registered in official statistics. It has been used successfully in many community-based...

Natrajan, P.K.; Muldoon, T.G.; Greenblatt, R.B.; Mahesh, V.B., 1981: Estradiol and progesterone receptors in estrogen-primed endometrium. Estradiol and progesterone receptor levels were measured in endometrial samples obtained from patients who were on different dosages of estradiol therapy and from women in the late proliferative phase of a normal menstrual cycle. Samples of blood...

Sarmehmetoglu, O.; Bumn, A.; Gonenc, B., 2004: Diagnosis of secondary hydatid cysts in white mice by ultrasonography and Doppler examination. Experimental hydatid cyst formation via inoculation of laboratory animals with Echinococcus protoscoleces is a widely used technique because it eliminates the dangers of accidental infection risks during working with Echinococcus eggs. It is easy...

Paschke, A.; Schwab, K.; Brümmer, J.; Schüürmann, G.; Paschke, H.; Popp, P., 2006: Rapid semi-continuous calibration and field test of membrane-enclosed silicone collector as passive water sampler. The new membrane-enclosed silicone collector (MESCO) was, in two different configurations with respect to the thickness of low-density polyethylene membrane used, subject to serial batch extraction tests to obtain (preliminary) sampling rates for...