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Efeito de diferentes concentracoes do inibidor da sintese de oxido nitrico na maturacao nuclear in vitro de oocitos bovinos


, : Efeito de diferentes concentracoes do inibidor da sintese de oxido nitrico na maturacao nuclear in vitro de oocitos bovinos. Revista Brasileira de Reproducao Animal 26(3): 149-151

The aim of the present study was to evaluate the effects of different concentration of NO synthesis inhibitor (Nomega-nitro-L-arginina metil ester, L-NAME) on the nuclear maturation bovine oocyte in vitro The cumulus oocyte complexes were cultured with L-NAME (0.01, 0.5 and 1.0 mM) for 24 hours. After this period, the oocytes were fixed and stained with orcein 2% to determine nuclear maturation stage.

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