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Effect of (auto)phosphorylation on the kinetic behavior of the Ca2+/calmodulin-dependent protein kinase II from horse brain

, : Effect of (auto)phosphorylation on the kinetic behavior of the Ca2+/calmodulin-dependent protein kinase II from horse brain. Biochimie 84(7): 605-610

After extraction and purification, the kinetic behavior of the Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) from horse brain was investigated as a function of ATP and a synthetic substrate, syntide-2. Both phospho- and dephospho- forms of the enzyme obey a bi-bi random mechanism. The KMs for ATP (KM,ATP) and syntide-2 (KM,syntide-2) were determined as equal to 80 and 30 muM, respectively.


PMID: 12453632

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