geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effect of 1,25-dihydroxyvitamin D3 on cultured mesencephalic dopaminergic neurons to the combined toxicity caused by L-buthionine sulfoximine and 1-methyl-4-phenylpyridine


, : Effect of 1,25-dihydroxyvitamin D3 on cultured mesencephalic dopaminergic neurons to the combined toxicity caused by L-buthionine sulfoximine and 1-methyl-4-phenylpyridine. Journal of Neuroscience Research 62(3): 374-382, November 1

A decrease in intracellular glutathione content may be related to the primary event in Parkinson's disease, so increasing the glutathione level may have a therapeutic benefit. The biologically active form of vitamin D, 1,25-dihydroxyvitamin D(3) [1, 25-(OH)(2)D(3)] has been recently reported to enhance the intracellular glutathione concentration in the central nervous system. Exposing rat cultured mesencephalic neurons for 24 hr to a mixture of L-buthionine sulfoximine (BSO) and 1-methyl-4-phenylpyridium ions (MPP(+)) resulted in a relatively selective damage to dopaminergic neurons. This damage has been accompanied by a reduction of intracellular glutathione levels. Low doses, i.e., 1-100 nM, of 1,25-(OH)(2)D(3) protect cultured dopaminergic neurons against this toxicity, although higher concentrations of this active form of vitamin D have been found to enhance the toxic effect. Generation of reactive oxygen species (ROS) by this toxicity has been attenuated in cultures being pretreated with low concentrations of 1,25-(OH)(2)D(3). Because the hormone increases the intracellular glutathione content in cultures, determining how this hormone suppresses ROS generation may involve the enhancement of the antioxidative system. These data suggest that low doses of 1,25-(OH)(2)D(3) are able to protect mesencephalic dopaminergic neurons against BSO/MPP(+)-induced toxicity that causes a depletion in glutathione content.

US$19.90

PMID: 11054806

DOI: 10.1002/1097-4547(20001101)62:3<374::aid-jnr7>3.0.co;2-7


Other references

Melton, D.W.; McEwan, C.; McKie, A.B.; Reid, A.M., 1986: Expression of the mouse HPRT gene: deletional analysis of the promoter region of an X-chromosome linked housekeeping gene. The mouse hypoxanthine phosphoribosyltransferase gene, like several other housekeeping genes, lacks many of the features associated with promoters of RNA polymerase II-transcribed genes. HPRT transcripts have multiple initiation sites and an HPRT...

Chang, C.F.ng; Jeng, S.R.; Lin, M.N.n; Tin, Y.Y.en, 1996: Purification and characterization of vitellin from the mature ovaries of prawn, Penaeus chinensis. Purification and the characterization of ovarian vitellin of mature female prawn, Penaeus chinensis, were the objectives of this study. Mature ovaries were homogenized and purified with gel filtration, hydroxylapatite, polyacrylamide gel electroph...

White J.D.; Perkins D.W.; Taylor S.I., 1973: Biosynthesis of ergosta 4 6 8 14 22 tetraen 3 one a novel oxygenative pathway. Bioorganic Chemistry 2(2): 163-175

Schwartz, D.T., 2009: Sensitivity of computed tomography for subarachnoid hemorrhage. Annals of Emergency Medicine 53(1): 160-1; Author Reply 162

Kuźnicki, J., 1986: Myosin phosphorylation in vivo and in vitro. II. Muscle myosin. Postepy Biochemii 32(1-2): 35-57

Butlin, J.A., 1971: The effect of Canadian business cycles on the adoption of technological innovations in Canadian agriculture 1926/1967. This paper is concerned with inter-relationships between the farm and non-farm sectors of advanced economies particularly the timing of adoption of innovations in non-farm-produced agricultural inputs relative to conditions in the non-farm economy...

Hammersen, F.; Amon, T., 1968: Elektronenmikroskopische Beobachtungen an Dotterplattchee in verschiedenen Zelltypen von Kaulquappen (Rana temporaria). Verhandlungen der Anatomischen Gesellschaft, 121: 441 -447

Jones, R.M.; Searle, J.B., 2003: Mapping the course of the Oxford-Hermitage chromosomal hybrid zone in the common shrew Sorex araneus: A GIS approach. The Oxford and Hermitage chromosomal races occupy relatively exclusive areas in Britain, and it is of interest to understand the factors that determine the location of the hybrid zone that lies between them. Within a Geographic Information System...

Cifuentes Delatte, L., 1974: History of prostatectomy. A brief developmental scheme. Anales de la Real Academia Nacional de Medicina 91(1): 43-62

Meirovitch E.; Luz Z.; Kalb A.J., 1974: Esr spectroscopy of aqueous solutions of concanavalin a. Journal of the American Chemical Society 96(24): 7542-7546