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Effect of 1-chloro-2,4-dinitrobenzene on K+ transport in normal and sickle human red blood cells


, : Effect of 1-chloro-2,4-dinitrobenzene on K+ transport in normal and sickle human red blood cells. Journal of Physiology (Cambridge) 547(3): 903-911, 15 March

1-Chloro-2,4-dinitrobenzene (CDNB), which causes oxidative stress through depletion of reduced glutathione (GSH), increases the passive K+ permeability of red cells. In this paper, we investigated the effects of CDNB (1 mM) on the activities of the K+-Cl- cotransporter (KCC; measured as Cl--dependent K+ influx) and the Gardos channel (taken as clotrimazole-sensitive K+ influx, 5 microM) in human red cells, using 86Rb+ as a K+ congener. 45Ca2+ was used to study passive Ca2+ entry and active Ca2+ efflux via the plasma membrane Ca2+ pump. Both the Gardos channel and KCC were stimulated in both normal and sickle red cells. In sickle cells, stimulation of KCC was similar in oxygenated and deoxygenated cells; that of the Gardos channel was greater in deoxygenated cells. In normal red cells, stimulation of both pathways was greater in oxygenated cells (by 4 +/- 1-fold; all means +/- S.E.M., n = 3). The effects on the Gardos channel were dependent on extracellular Ca2+ and were associated with inhibition of the plasma membrane Ca2+ pump (by 29 +/- 3 %, P < 0.01) and increased Ca2+ sensitivity of the channel (EC50 for [Ca2+]i reduced from 260 +/- 26 to 175 +/- 15 nM; P < 0.05). Cell volume, pHi, ATP levels and passive Ca2+ entry were not affected by CDNB. The effects on KCC were inhibited (93 +/- 6 %) by prior treatment with the protein phosphatase inhibitor calyculin A (100 nM) and were not additive with stimulation by N-ethylmaleimide (1 mM), regardless of the order of addition. These findings are therefore consistent with inhibition of a regulatory protein kinase, although stimulation of the conjugate protein phosphatase(s) may also occur. KCC stimulation was also Ca2+ dependent. These findings are important for understanding how GSH depletion alters membrane permeability and how to protect against red cell dehydration.

US$19.90

PMID: 12576491

DOI: 10.1113/jphysiol.2002.036467


Other references

Kotilainen, Mauno J., 1944: Promemoria Fennoskand-ian kasvimaantieteellisesta kaakkoisrajasta ja samalla Aunuk-sen Karjalan etelarajasta Aunuksen kaunaksella. Memoranda Soc Fauna Et Flora Fennica 19: 180-192, 1943 ( )

Charteris, D.G., 1997: Surgical management of HIV related retinal detachment. British Journal of Ophthalmology 81(3): 177-178

Swank, R.T.; Burris, R.H., 1969: Purification and properties of cytochromes c of Azotobacter vinelandii. 1.1. An improved method for extraction and purification of Azotobacter vinelandii cytochromes c by chromatography with DEAE-cellulose and Sephadex and by isoelectric focusing is described. Yields of purified cytochromes c4 and c5 were approximatel...

Miyoshi, K., 1995: Influences of tooth contact patterns during mandibular retrusion on the terminal jaw relation of habitual closures. Influence of tooth contact patterns during mandibular retrusion on the terminal jaw relation (TJR) of habitual closures were investigated on five subjects. A maxillary stabilization splint with steep retrusive contacts was fabricated and applied t...

Lloyd, R.E.; Badia, E.; Fazeli, A.; Watson, P.F.; Holt, W.V., 2008: Temporal dynamics of ram sperm binding and survival during 48-h coculture with oviducal epithelial cells. Following insemination, ram spermatozoa bind to oviducal epithelial cells (OEC) <i>in vivo</i> and remain viable for several hours before fertilisation. In the present study, we investigated whether OEC monolayers reproduce this effect...

Marchetti, M.; Tolomelli, B.; Formiggini, G.; Bovina, C.; Barbiroli, B., 1980: Distribution of pteroylglutamates in rat liver during regeneration after partial hepatectomy. 1. Liver pteroylpolyglutamate distribution was studied during regeneration after partial hepatectomy in rats maintained under controlled feeding conditions. 2. Pteroylhexaglutamate, pteroylpentaglutamate and pteroyltetraglutamate concentrations de...

Brown, J.M., 1997: Re-extraction HBO: empiricism or science?. Journal of Oral and Maxillofacial Surgery 55(10): 1193-1193

Venturi, A.; Simoni, A., 1995: Energy production due to swine manure processes. Equipment and methodology for measuring swine manure energy production are described. Energy unit production is sensible and can be considered useful for energy-balance in buildings for straw-litter swine breeding.

Debruck, J.; Vomel, A., 1972: Utilization of nutrients in sewage sludge. In field and pot experiments, 15-30% of the N in sewage sludge was utilized by field crops, but grassland utilized <15%. K was utilized completely.

Boddie, J.; Whitcomb, C.E., 1978: Plant modification with a mutagenic compound. Seeds of Lagerstroemia indica and Maclura pomifera treated with ethyl methanesulfonate produced some mutants of possible ornamental value.