geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effect of 17beta-estradiol and testosterone on the expression of flavin-containing monooxygenase and the toxicity of aldicarb to Japanese medaka, Oryzias latipes


, : Effect of 17beta-estradiol and testosterone on the expression of flavin-containing monooxygenase and the toxicity of aldicarb to Japanese medaka, Oryzias latipes. Toxicological Sciences 68(2): 381-388

Previous studies in our laboratory indicated gender differences in salinity-enhanced acute toxicity of aldicarb in Japanese medaka with females being more susceptible. In the current study, the effects of the sex steroids, 17beta estradiol (E2) and testosterone (T) on aldicarb toxicity was examined. Adult Japanese medaka were separated by sex and exposed to 100 microg/l E2 or T for 6 days followed by exposure to the 96-h LC50 (0.5 mg/l) of aldicarb. The toxicity of aldicarb to adult males was significantly lowered by E2 and T whereby the mortality percentage was reduced to 23.3 +/- 5.8% and 3.3 +/- 5.8%, respectively, compared to the fish not receiving steroids (46.7 +/- 5.8% mortality). In females, T caused significant reduction in aldicarb toxicity to 16.7 +/- 5.8%, while E2 significantly enhanced the toxicity to 96.7 +/- 5.8% mortality. Since the flavin-containing monooxygenase (FMO) enzyme system had been shown to play a critical role in aldicarb toxicity, the effect of E2 and T on FMO expression was examined. Gill FMO activity showed a direct correlation with the overall toxicity of aldicarb in both male and female medaka. Expression of FMO1-like protein was significantly reduced by T in male livers and gills, and T did not affect the expression of FMOs in female tissues. In contrast, E2 significantly reduced FMO1-like protein expression in male gills and female livers, as well as FMO3 expression in both male and female livers, but significantly increased gill FMO1 expression in females. Since aldicarb acts by inhibiting the enzyme cholinesterase (ChE), the effect of sex hormones on the activity of this enzyme was also examined. In both male and female medaka, T counteracted the inhibitory effect of aldicarb on muscle ChE. In male fish, E2 had similar effects but did not seem to counteract the ChE inhibition in females. In conclusion, E2 and T modulation of aldicarb toxicity in Japanese medaka seems to be mediated via alteration of gill FMO and ChE actitivies.

US$19.90

PMID: 12151634

DOI: 10.1093/toxsci/68.2.381


Other references

Gorbachkova E.A., 1970: A study of the mechanism of the effect of strophanthin k on interferonogenesis in a cellular culture. Byulleten' Eksperimental'Noi Biologii I Meditsiny: 66-69

Misra, M.; Ashworth, J.; Reaveley, D.A.; Muller, B.; Brown, E.A., 1996: Nutritional effects of amino acid dialysate (Nutrineal) in CAPD patients. The use of amino acid dialysate (AAD) has been shown to improve the nutritional status of malnourished continuous ambulatory peritoneal dialysis (CAPD) patients. We report on a randomized, prospective, cross-over study evaluating the effects of a...

Bertrand, Bît.; Stefan, L.; Pirrotta, M.; Monchaud, D.; Bodio, E.; Richard, P.; L.G.ndre, P.; Warmerdam, E.; de Jager, M.H.; Groothuis, G.M.M.; Picquet, M.; Casini, A., 2014: Caffeine-based gold(I) N-heterocyclic carbenes as possible anticancer agents: synthesis and biological properties. A new series of gold(I) N-heterocyclic carbene (NHC) complexes based on xanthine ligands have been synthesized and characterized by mass spectrometry, NMR, and X-ray diffraction. The compounds have been tested for their antiproliferative propertie...

Huang, J-Fu., 2013: Pragmatic solution for organ donation in response to challenges faced by the Chinese society: summary for the National Donation after Circulatory Death Pilot Program. Chinese Medical Journal 126(3): 569-573

Bebneva, L.B.; Boichenko, A.E.; Gul'yants, E.S., 1978: Histo pathology of the cardio vascular system during acute hemorrhage as affected by intra arterial re infusion. The significance of the pressor factor in the development of histopathological changes in the cardiovascular system during acute hemorrhage was studied in 20 dogs in a model of acute hemorrhage and intraarterial reinfusion. The morphofunctional in...

Amagasaki, T.; Momita, S.; Suzuyama, J.; Yamada, Y.; Ikeda, S.; Kinoshita, K.; Ichimaru, M., 1985: Morphological examination of lymphocytes from human T-cell leukemia virus (HTLV) carriers. Japanese Journal of Clinical Hematology 26(9): 1430-1435

Mayer, V.; Kozuch, O., 1969: Study of the virulence of tick-borne encephalitis virus. XI. Genetic heterogeneity of the virus from naturally infectious bodes ricinus ticks. The genetic heterogeneity of virus strains freshly isolated from ticks was in contrast to the homogeneity of strains maintained in the lab. The effect of a single passage through mouse brain was studied by means of a genetic marker analysis techni...

Serra G.; Lartaud G.; Marchand D., 1973: Use of tridemorph against cereal mildew erysiphe graminis in france. Mededelingen van de Faculteit Landbouwwetenschappen Universiteit Gent 38(3 (PART 2)): 1585-1594

Ladurner, R.; Traub, F.; Schenk, M.; Königsrainer, A.; Glatzle, Jörg., 2009: Cellular liver regeneration after extended hepatic resection in pigs. The liver has an enormous capacity to regenerate itself. The aim of this study was to evaluate whether the regeneration is due to hypertrophy or hyperplasia of the remnant liver after extended resection and whether a portosystemic shunt is benefic...

Dunahay, T.G.; Jarvis, E.E.; Dais, S.S.; Roessler, P.G., 1996: Manipulation of microalgal lipid production using genetic engineering. Genetic transformation of two species of diatoms has been accomplished by introducing chimeric plasmid vectors containing a bacterial antibiotic resistance gene driven by regulatory sequences from the acetyl-CoA carboxylase (ACCase) gene from the...