geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effect of 17beta-estradiol on intracellular Ca(2+) levels in renal tubular cells


, : Effect of 17beta-estradiol on intracellular Ca(2+) levels in renal tubular cells. Pharmacology 64(2): 84-90

The effect of 17beta-estradiol on intracellular Ca(2+) concentrations ([Ca(2+)](i)) in Madin Darby canine kidney cells was investigated by using the fluorescent dye fura-2. 17Beta-estradiol (5-100 micromol/l) induced instantaneous increases in [Ca(2+)](i) in a concentration-dependent manner. Ca(2+) removal inhibited 45 +/- 15% of the Ca(2+) signal. In Ca(2+)-free medium, pretreatment with 50 micromol/l 17beta-estradiol abolished the [Ca(2+)](i) increases induced by 2 micromol/l carbonylcyanide m-chlorophenylhydrazone (CCCP; a mitochondrial uncoupler), 1 micromol/l thapsigargin (an endoplasmic reticulum Ca(2+) pump inhibitor) and 50 micromol/l brefeldin A (an antibiotic which disperses the Golgi complex), but pretreatment with brefeldin A, CCCP and thapsigargin only partly inhibited the 17beta-estradiol-induced [Ca(2+)](i) signal. Adding 3 mmol/l Ca(2+) increased [Ca(2+)](i) in cells pretreated with 5-100 micromol/l 17beta-estradiol in Ca(2+)-free medium. Pretreatment with 1 micromol/l U73122 to abolish the formation of inositol-1,4,5-trisphosphate inhibited 50% of the Ca(2+) release induced by 50 micromol/l 17beta-estradiol. 17Beta-estradiol (20 micromol/l) also increased [Ca(2+)](i) in human bladder cancer cells and prostate cancer cells. Collectively, this study shows that 17beta-estradiol evoked a significant internal Ca(2+) release and external Ca(2+) entry possibly in a nongenomic manner.

US$29.90

PMID: 11803248


Other references

Ichikawa, M.; Ide, N.; Ono, K., 2006: Changes in organosulfur compounds in garlic cloves during storage. We determined the changes in the contents of three (Sd(B-glutamyl peptides and four sulfoxides in garlic cloves during storage at -3, 4, and 23 degrees C for 150 days using a validated high-performance liquid chromatography method that we reported...

Park, S.; Hong, S.M.; Lee, J.E.; Sung, S.R.; Kim, S.H., 2008: Chlorpromazine attenuates pancreatic beta-cell function and mass through IRS2 degradation, while exercise partially reverses the attenuation. We investigated the effect and mechanism of exercise and chlorpromazine (CPZ), a conventional anti-psychotic drug, on beta-cell function and mass in 90% pancreatectomized (Px) male rats. The diabetic Px rats were divided into two groups, one of wh...

Morris, K.R.; Lutz, R.D.; Bai, X.; McGibney, M.T.; Cook, D.; Ordway, D.; Chan, E.D., 2010: Suppression of IFNgamma+mycobacterial lipoarabinomannan-induced NO by IL-4 is due to decreased IRF-1 expression. In mice, and possibly in humans, nitric oxide (NO) is an important host-defense molecule against Mycobacterium tuberculosis. Inducible nitric oxide synthase (iNOS) and NO are upregulated in murine macrophages stimulated with interferon-gamma (IFNg...

Shenfield, G.M.; Hodson, M.E.; Clarke, S.W.; Paterson, J.W., 1975: Patterns of response to isoprenaline in asthmatic patients. Six asthmatic patients whose airway obstruction improved significantly during a trial of prednisone have been studied in detail. Serial measurements of forced expiratory volume in i second (FEV1) were made at specific times each day before and aft...

Gröning, F.; Fagan, M.J.; O'Higgins, P., 2011: The effects of the periodontal ligament on mandibular stiffness: a study combining finite element analysis and geometric morphometrics. It is generally accepted that the periodontal ligament (PDL) plays a crucial role in transferring occlusal forces from the teeth to the alveolar bone. Studies using finite element analysis (FEA) have helped to better understand this role and show...

Bal, J.; Kundalgurki, S., 1999: Tooth sensitivity prevention and treatment. Oral Health 89(2): 33-4, 37-8, 41

Blake, A.M.; Langham, J.; Collins, L.M.; Reynolds, E.O., 1970: Clinical assessment of apnoea-alarm mattress for newborn infants. Lancet 2(7665): 183-185

Caselli, R., 1975: Nutritional factors in swine raising. Suinicoltura 16 (10) 41-54

Hull, R., 1987: Letter from Amsterdam: Flood or pestilence. British Medical Journal 295(6597): 539-540

Zelante, L.; Notarangelo, A.; Croce, A.I.; Piemontese, M.R.; Gasparini, P., 1994: Cytogenetic and molecular analysis of trisomy 9. Case report and review. A newborn infant with the full manifestations of trisomy 9 syndrome is reported. Cytogenetic analysis reveled an homogeneous aneuploidy. Molecular studies using polymorphic microsatellites of chromosome 9 showed that non disjunction occurred at ma...