+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effect of methyl-beta-cyclodextrin and fertilization promoting peptide on capacitation of boar spermatozoa in a protein-free medium

, : Effect of methyl-beta-cyclodextrin and fertilization promoting peptide on capacitation of boar spermatozoa in a protein-free medium. Journal of Reproduction & Development 48(1): 57-63, February

The present study was undertaken to examine the effect of methyl-beta-cyclodextrin (MBCD) and fertilization promoting peptide (FPP; pGlu-Glu-ProNH2) on the capacitation and acrosome reaction of boar spermatozoa in a protein-free medium. After washing with a protein-free medium, ejaculated spermatozoa (resuspended at 1 X 106 cells/ml) were cultured in 2 ml of modified Medium-199 containing polyvinylalcohol (mM199-PVA) supplemented or not supplemented with MBCD and/or FPP for 2 h in an atmosphere of 5% CO2 in air at 39 C. Effects of MBCD and FPP on boar spermatozoa were determined by chlortetracycline fluorescence assessment. When spermatozoa were cultured in various MBCD concentrations, capacitation was induced in a concentration-dependent manner, but spontaneous acrosome reaction was not affected. When the effect of FPP in mM199-PVA was determined, sperm capacitation was induced and spontaneous acrosome reaction was inhibited in 100 nM FPP, regardless of the absence or presence of BSA. In the last experiment, spermatozoa were cultured in mM199-PVA supplemented with various concentrations of MBCD and/or FPP. An accelerative effect of MBCD on sperm capacitation was observed in a concentration-dependent manner even in the presence of FPP, whereas 1-2 mM of MBCD neutralized the stimulatory effect of FPP on capacitation. Furthermore, FPP inhibited spontaneous acrosome reaction even in the presence of MBCD. These results demonstrate that MBCD and PFF can induce capacitation of boar spermatozoa in a protein-free medium and that FPP alone also inhibits spontaneous acrosome reaction.


Other references

Gaeke, R.; Gaeke, M.E., 1979: Filing systems for medical literature. JAMA 242(7): 615-616

Xu, Y-Tong.; Hon, P-Ming.; Jiang, R-Wang.; Cheng, L.; Li, S-Hong.; Chan, Y-Pong.; Xu, H-Xi.; Shaw, P-Chui.; But, P.Pui-Hay., 2006: Antitussive effects of Stemona tuberosa with different chemical profiles. The root tubers of Stemona tuberosa, Stemona japonica and Stemona sessilifolia are recognized by the Pharmacopoeia of the People's Republic of China as authentic sources of the herb Radix Stemonae (Baibu). Careful anatomical analyses of these...

Kelsey, K.T.; Caggana, M.; Mauch, P.M.; Coleman, C.N.; Clark, J.R.; Liber, H.L., 1993: Mutagenesis after cancer therapy. A subset of Hodgkin's disease (HD) and breast cancer patients have been reported to have elevated hprt mutant frequencies in peripheral blood lymphocytes after cessation of therapy. A subset of these patients are also known to develop second...

Harvey H.R.dger; Patton John S., 1983: Lipid composition and bacterial distribution in sediments of the Venezuela Basin. Eos, Transactions, American Geophysical Union 64(52): 1085

McGinnis Thomas L.; Weisbrod Noam; Selker John S.; Niemet Michael R., 2000: Infiltration of highly concentrated solutions in layered unsaturated sand. Eos, Transactions, American Geophysical Union 81(48, Suppl

Park, T.S.; Alejar, A.A.; Vergara, B.S., 1992: Flag leaf photosynthesis and senescence of the upper three leaves during spikelet filling in rice. Murata, N [Editor] Research in photosynthesis, Vol IV : 707-708

Asselbergs, E., 1912: Description des Fossiles decouverts par M. J. Duvigneaud aux environs de Neufchateau. Bulletin Soc Geol Paleont Hydr Bruxelles, 26 189-215

Zekâi ?en, 1980: Regional drought and flood frequency analysis; theoretical consideration. The problem of regional drought and flood analysis has been approached theoretically on the basis of random fields. Necessary formulations for the regional drought descriptors such as the deficit area, the total areal deficit and the maximum defic...

Juurlink, B.H.J., 1998: The multiple sclerosis lesion: Initiated by a localized hypoperfusion in a central nervous system where mechanisms allowing leukocyte infiltration are readily upregulated?. A mechanism is proposed that may explain the factors that initiate a multiple sclerosis (MS) lesion. It is based upon the following two hypotheses: (i) there is a lower stimulus threshold for upregulating the mechanisms that result in leukocyte in...

Gerdeaux, D.; Jestin, J-M., 1979: Exemple d'application du chalut electrifie dans un milieu tempere tres mineralise. Annales de Limnologie. 1978 ; 143: 281-287