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Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss)

, : Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss). Environmental Toxicology and Chemistry 21(3): 557-560

As part of an effort to validate the use of heat shock proteins (HSPs) as biomarkers of exposure to and effects of contaminants, we evaluated the effect of two handling regimens on the induction of HSP 60 and 70 in rainbow trout (Oncorhynchus mykiss). Fish were acclimated to laboratory conditions for several weeks before the beginning of the experiment. Fish were then captured by net, placed in a cooler for 1 h while being transported in a truck, returned to their original tanks, then sacrificed 6 to 8 h later. Tricaine methane sulfonate (MS‐222) was used during different phases of handling to reduce handling stress. Heat‐stressed fish were included in the experiment as a positive control. Muscle, liver, gills, and heart were analyzed for HSP 60 and 70 by immunoblotting. We found no effect of any handling regimen on the induction of HSPs. These findings suggest that the capture and transport of fish for environmental monitoring purposes should not interfere with the use of stress proteins as biomarkers.


PMID: 11878469

DOI: 10.1002/etc.5620210313

Other references

Sen, N.K.; Chakravarti, J.K., 1953: Corchorgenin-a cardiac principle from Corchorus olitorius Linn. Sci And Culture 19(2): 102-103

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Axelsson, J., 1931: Undersokningar rorande honsaggens Klackningsvalitet. Acta Univ: 1-44

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Uskokovic M.R.; Baggiolini E.; Batcho A.; Shiuey S J.; Wovkulich P.; Horst R.L., 1988: Synthesis of 26 27 hexadeutero and epimeric 26 trideutero 1 25 dihydroxycholecalciferols. Norman, A W , Et Al (Ed ) Vitamin D: Molecular, Cellular And Clinical Endocrinology; Seventh Workshop on Vitamin D, Rancho Mirage, California, Usa, April 24-29, Xxxix+1071p Walter De Gruyter And Co : Berlin, West Germany; New York, New York, Usa Illus 22-24