geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of solution polarity and viscosity on peptide deamidation


, : Effects of solution polarity and viscosity on peptide deamidation. Journal of Peptide Research 56(5): 326-334, November

Deamidation kinetics were measured for a model hexapeptide (L-Val-L-Tyr-L-Pro-L-Asn-Gly-L-Ala, 0.02 mg/mL) in aqueous solutions containing glycerol (0-50% w/w) and poly(vinyl pyrrolidone) (PVP, 0-20% w/w) at 37degreeC and pH 10 to determine the effects of solution polarity and viscosity on reactivity. The observed pseudo-first order deamidation rate constants, kobs, decreased markedly when the viscosity increased from 0.7 to 13 cp, but showed no significant change at viscosities >13 cp. Values of kobs also increased with increasing dielectric constant and decreasing refractive index. Molecular dynamics simulations indicated that the free energy associated with Asn side-chain motion is insensitive to changes in dielectric constant, suggesting that the observed dielectric constant dependence is instead related primarily to the height of the transition state energy barrier. An empirical model was proposed to describe the effects of the viscosity, refractive index and dielectric constant on kobs. Analysis of the regression coefficients suggested that both permanent and induced dipoles of the medium affect the deamidation rate constant, but that solution viscosity is relatively unimportant in the range studied.

US$19.90

PMID: 11095186

DOI: 10.1034/j.1399-3011.2000.00783.x


Other references

Chilton, S.J.P.; Moreland, C.F., 1954: Experiments on the flowering of sugarcane. Some plants of the varieties CP 29-116, 34-120, 36-13, 36-105 and 44-101 and Co. 290 were subjected in May to dark periods which were gradually lengthened and other plants were similarly treated in August. Floral initials were formed by the plants...

Walker, B.A.; Mehr, M.; Brummel-Smith, K.; Kahn, E., 1982: Effects of behavioral science orientations on attitudes of first-year residents in family practice. Journal of Medical Education 57(11): 877-879

Mihăilă, I., 1981: Ergonomics as a cybernetics system of the "man - production" relationships. La Sante Publique 24(2-3): 297-301

Evans, D.Gareth.R.; Lennard, F.; Pointon, L.J.; Ramus, S.J.; Gayther, S.A.; Sodha, N.; Kwan-Lim, G.E.; Leach, M.O.; Warren, R.; Thompson, D.; Easton, D.F.; Eeles, R., 2009: Eligibility for magnetic resonance imaging screening in the United Kingdom: effect of strict selection criteria and anonymous DNA testing on breast cancer incidence in the MARIBS Study. A UK multicenter study compared the performance of contrast enhanced-magnetic resonance imaging with X-Ray Mammography in women at high-risk of breast cancer commencing in 1997. Selection criteria were used to identify women with at least 0.9% ann...

Farrerons J., 1987: Will recent advances in the field of osteoporosis change our therapeutic approach?. Revista Espanola de Reumatologia 14(6): 237-239

Ratcliffe, Ej, 1977: Traffic casualties. Barn owls on the decline. Wildlife, Lond9(8): 362-363,illust.

Bianchi, V.; Premaschi, S.; Raspagni, A.; Secco, S.; Vidali, M., 2016: A comparison between serum carbohydrate-deficient transferrin and hair ethyl glucuronide in detecting chronic alcohol consumption in routine. In heavy alcohol consumption laboratory tests represent an objective evidence. In this study we compared older and newer biomarkers in blood and in hair. Carbohydrate-deficient transferrin (CDT), ethyl glucuronide (EtG), AST, ALT, GGT, MCV were me...

Jones, K.W.; Cannon, J.B.; Saavedra, F.A.; Kampf, S.K.; Addington, R.N.; Cheng, A.S.; MacDonald, L.H.; Wilson, C.; Wolk, B., 2017: Return on investment from fuel treatments to reduce severe wildfire and erosion in a watershed investment program in Colorado. A small but growing number of watershed investment programs in the western United States focus on wildfire risk reduction to municipal water supplies. This paper used return on investment (ROI) analysis to quantify how the amounts and placement of...

Venzlaff, U., 1967: Suicide as a human, medical, and health-political problem. Der Landarzt 43(21): 989-998

Warnock, D.W., 1992: Fungal infections. Fungal infections remain important opportunistic pathogens, particularly in immunocompromised individuals. A variety of fungal pathogens have been identified that cause infection in the growing population of immunocompromised patients. Over the la...