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Engagement of CD99 induces apoptosis through a calcineurin-independent pathway in Ewing's sarcoma cells


, : Engagement of CD99 induces apoptosis through a calcineurin-independent pathway in Ewing's sarcoma cells. American Journal of Pathology 153(6): 1937-1945

Programmed cell death (PCD) is a prominent feature of the development of the immune and nervous systems. In both systems, widespread PCD occurs in primitive progenitor cells during development. In this study, we demonstrated that Ewing's sarcoma (ES) cells, undifferentiated neural precursors, underwent apoptosis upon engagement of CD99 with anti-CD99 monoclonal antibody. Apoptosis via CD99 occurred only in the undifferentiated state of ES cells, but not in differentiated ES cells. CD99-induced apoptosis in ES cells appeared to require de novo synthesis of RNA and protein as well as caspase activation. Cyclosporin A, known to be a potent inhibitor of both calcineurin activation and mitochondrial permeability transition pore opening, inhibited CD99-mediated apoptosis, whereas FK-506, a specific calcineurin inhibitor, did not, indicating the induction of CD99-mediated apoptosis through a calcineurin-independent pathway. Furthermore, the dying cells displayed the reduction of mitochondrial transmembrane potential (delta psi m). These results suggest that CD99 engagement induce CsA-inhibitable mitochondrial permeability transition pore opening, followed by a reduction of delta psi m and caspase activation, thereby leading to apoptosis. Based on these results, we suggest the possible involvement of CD99 in the apoptotic processes that occur during nervous system development and also its application in immunotherapeutic trials for ES cases.

US$19.90

PMID: 9846983

DOI: 10.1016/S0002-9440(10)65707-0


Other references

Dresner, Edgar, 1953: Observations on the biology and habits of pupal parasites of the oriental fruit fly. Several species of Spalangia, Dirhinus, Psilus, Pachycrepoideus, and Trichopria were sent to Hawaii from Africa, the Orient and the Pacific for trial as parasites of the oriental fruit fly. Exposures of puparia parasitized by Oplus vandenboschi fa...

Richter H.P., 1987: Vitellogenesis in xenopus and bufo ultrastructure of yolk platelet membranes. Cell Differentiationppl: 54s

Lehmann, F., 1931: Fat metabolism. This review of fat metabolism, one of the most important subjects in animal nutrition, gives a historical survey of the theories of fat formation in the animal body. Of all foodstuffs, protein is the most doubtful precursor, but need not be comple...

Lee, J., 2013: Growth spurt. As patents expire, focus is on high-priced drugs. Modern Healthcare 43(4): 7, 16-7, 16

Duncan, B.; Schmidt, M.; Pankow, J.; Ballantyne, C.; Couper, D.; Vigo, A.; Hoogeveen, R.; Folsom, A.; Heiss, G., 2003: Low-grade systemic inflammation and the development of type 2 diabetes mellitus The ARIC Study. Diabetes 52(Supplement 1): A58

Aarons, D.H.; Rossi, G.V.; Orzechowski, R.F., 1977: Cardio vascular actions of 3 harmala alkaloids harmine harmaline and harmalol. Each of 3 harmala alkaloids [Peganum harmala] harmine, harmaline and harmalol, decreased heart rate and increased pulse pressure, peak aortic flow and myocardial contractile force in intact normotensive anesthetized dogs. Harmine reduced systemic...

Miller, G.A., 1933: Number of Operators of Prime Power Orders Contained in a Group. Proceedings of the National Academy of Sciences of the United States of America 19(8): 780-783

Flynn, M.D.; O'Brien, I.A.; Corrall, R.J., 1995: The prevalence of autonomic and peripheral neuropathy in insulin-treated diabetic subjects. The prevalence of autonomic and peripheral neuropathy was examined in 506 diabetic subjects treated with insulin, mean age 43 years, diabetes duration 15 (range 1-54) years. Autonomic neuropathy was present if two or more (of four) cardiovascular...

McAnulty, G.R.; Hall, G.M., 2003: Anaesthesia for the diabetic patient. British Journal of Anaesthesia 90(4): 428-429

Stamm, W.E.; Colella, J.J.; Anderson, R.L.; Dixon, R.E., 1975: Indwelling arterial catheters as a source of nosocomial bacteremia. An outbreak caused by Flavobacterium Species. Between mid-May and mid-October, 1973, 49 blood cultures from 14 patients in an intensive care unit were positive for flavobacterium species, Group II-b. We conducted an investigation to determine how patients were being infected with this unusual...