geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Enhanced proliferation of lymphoblasts from patients with Alzheimer dementia associated with calmodulin-dependent activation of the na+/H+ exchanger


, : Enhanced proliferation of lymphoblasts from patients with Alzheimer dementia associated with calmodulin-dependent activation of the na+/H+ exchanger. Neurobiology of Disease 8(2): 289-298

We have recently reported that lymphoblasts from late onset Alzheimer's disease (AD) patients show distinct intracellular pH homeostatic features than those obtained from age-matched healthy donors. Here we report that another distinct feature of AD lymphoblasts is their increased rate of proliferation in serum containing medium, suggesting a different responsiveness of AD cells to serum activators. The increased proliferation of AD cells was accompanied by intracellular alkalinization and was prevented by blockers of the plasma membrane Na+/H+ antiporter (NHE), indicating that the exchanger had to be activated to elicit the cellular responses. The activity of this exchanger can be controlled through several signaling pathways, but only the inhibition of calmodulin activity impeded the serum-induced intracellular alkalinization and enhanced proliferation of AD cells. In contrast, the inhibition of calmodulin did not alter the rate of proliferation of normal cells. Thus, it seems plausible to conclude that the enhanced proliferation of AD cells is the result of a surface receptor-mediated activation of the Ca(2+)-calmodulin signaling pathway. Our observations add further support in favor that AD may be considered a systemic disease which underlying etiopathogenic mechanism may be an altered responsiveness to cell activating agents. Thus, the use of lymphoblastoid cells from AD patients may be a useful model to investigate cell biochemical aspects of this disease.

US$19.90

PMID: 11300724

DOI: 10.1006/nbdi.2000.0381


Other references

Kachalova, L.M., 1983: Role of the pallidum in the formation of different types of adaptive behavior. Neuroscience and Behavioral Physiology 13(5): 337-345

Zhu, J.; Ding, Y.; Gao, F.; Wu, T.; Zhang, C.W.H.; Tien, P.; Rao, Z.; Gao, G.F., 2003: Crystallization and preliminary X-ray crystallographic analysis of the trimer core from measles virus fusion protein. Two heptad-repeat regions (HR1 and HR2) are highly conserved in paramyxovirus fusion proteins and form a stable helical trimer of heterodimers [(HR1-HR2)(3)] after the fusion between viral and cellular membranes. In this study, two HR regions of t...

Barker, A.K.; Troll, V.R.; Ellam, R.M.; Hansteen, T.H.; Harris, C.; Stillman, C.J.; Andersson, A., 2012: Magmatic evolution of the Cadamosto Seamount, Cape Verde; beyond the spatial extent of EM1. The Cadamosto Seamount is an unusual volcanic centre from Cape Verde, characterised by dominantly evolved volcanics, in contrast to the typically mafic volcanic centres at Cape Verde that exhibit only minor volumes of evolved volcanics. The magmat...

Dizdaroglu, M.; Simic, M.G.; Schott, H., 1980: Separation and sequencing of the sequence isomers of pyrimidine deoxypentanucleoside tetraphosphates by high-performance liquid chromatography. Chemical synthesis of DNA fragments with a defined sequence is extremely long and complex. A simpler alternative is isolation of fragments from partially hydrolysed DNA. Mixtures of sequence isomers of pyrimidine oligonucleotides were recently iso...

Hilderbrand, R.M.; Green, J.G., 1982: Resuscitating victims of head-neck carcinoma or trauma. Dental Assistant 51(3): 17-19

Wu, J-Qing.; Zhao, W-Hong.; Li, Y.; Zhu, B.; Yin, K-Sheng., 2007: Adeno-associated virus mediated gene transfer into lung cancer cells promoting CD40 ligand-based immunotherapy. Expression of the CD40 ligand (CD40L) on tumors can activate host immune systems and produce antitumor effects against the tumors. To deliver the CD40L gene efficiently, we evaluated the efficiency of transduction of different serotypes of adeno-a...

Anonymous, 1973: Reproductive function in the human male. Report of a WHO scientific group. World Health Organization Technical Report Series 520: 1-34

Springer, T.L., 1995: Seeding clovers into bermudagrass sod in the hill-lands of the western mid-south. Arkansas Agricultural Experiment Station Research Series 0(442): 33-37

Greenberg R.; Chapman C.R., 1981: Origin and derivation of stony-iron meteorites. Meteoritics 16(4): 320

Tjia, B.B.xton, J., 1973: Height control of poinsettias with A-Rest. Florists' Rev: 152 (3498) 24-25