geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Estimation of the abundance of the cadmium resistance gene cadA in microbial communities in polluted estuary water


, : Estimation of the abundance of the cadmium resistance gene cadA in microbial communities in polluted estuary water. Research in Microbiology 152(7): 671-678, September

We describe herein a molecular method for estimating the abundance of the cadA gene, which encodes a Cd2+/ATPase protein transporter, in bacterial DNA extracted from samples of environmental water. Competitive polymerase chain reaction (cPCR) may be the most appropriate technique for assessing the prevalence of the cadA gene in microbial communities in highly heterogeneous and polluted environments, such as the Seine estuary (France). We describe the development of this method: (i) the choice of two specific primers, based on the sequences encoding the cadmium binding site and the ion channel domains; (ii) the construction of a competitor sequence and assessment of its amplification efficiency; and (iii) the estimation of the copy number of the cadA gene. The cadA content in the bacterial community is expressed as the number of gene copies per ng of total DNA extracted, which is independent of the DNA extraction yield. This molecular procedure was improved to analyze cadA levels in bacterial DNA extracted from estuary water accidentally contaminated with cadmium. Results revealed a subsequent increase in the copy number of the cadA gene in the microbial community.

US$19.90

PMID: 11605987

DOI: 10.1016/s0923-2508(01)01246-3


Other references

Dalianis, C.D., 1980: Effect of temperature and seed size on speed of germination, seedling elongation and emergence of berseem and Persian clovers (Trifolium alexandrinum and T. resupinatum). Opt. temp. for rapid germination, emergence and seedling elongation of berseem and Persian clover were determined in conjunction with studies of the effect of seed size and sowing depth. Opt. temp. ranges for rapid mobilization of seed reserves an...

Taverner, P.A., 1918: The Hawks of the Canadian Prairie Provinces in theis relation to agriculture. Ottawa Museum Bulletin, 28 1-14

Wadsworth, F.B.; Vasseur, Jérémie.; Llewellin, E.W.; Dingwell, D.B., 2017: Sintering of polydisperse viscous droplets. Sintering-or coalescence-of compacts of viscous droplets is driven by the interfacial tension between the droplets and the interstitial gas phase. The process, which occurs in a range of industrial and natural settings, such as the manufacture of...

McKee, M., 1994: On advising politicians. Bmj 308(6940): 1313-1314

White, K.; Teal, S.B.; Potter, J.E., 2015: Contraception after delivery and short interpregnancy intervals among women in the United States. To investigate women's patterns of contraceptive use after delivery and the association between method use and risk of pregnancy within 18 months. We used the 2006-2010 National Survey of Family Growth to examine women's contraceptive us...

Van Niekerk, B.D.H.; Basson, W.D.; Mulder, A.M., 1967: Influence of frequency of feeding and of a supplement of urea on sheep in conditions of drought. A group of 10 mature wethers in one trial of 9 weeks and of 6 ewes in another of 7 weeks, all about 70 lb liveweight at the start, grazing on Karoo veld during drought, were given 0.57 lb yellow maize daily per head. Similar groups had the same to...

Speirs, A.D.; Beaulé, P.E.; Rakhra, K.S.; Schweitzer, M.E.; Frei, H., 2013: Increased acetabular subchondral bone density is associated with cam-type femoroacetabular impingement. Femoro-acetabular impingement (FAI) has been associated with significant acetabular cartilage damage and subsequent degenerative arthritis. Subchondral bone, often neglected in osteoarthritis studies, may play an important role in the degenerative...

Brack A., 1986: Emergence and survival of protein like peptides in water. Origins of Life 16(3-4): 486

Shaymardanova, G.F.; Mukhamedshina, Y.O.; Salafutdinov, I.I.; Rizvanov, A.A.; Chelyshev, Y.A., 2013: Usage of plasmid vector carrying vegf and fgf2 genes after spinal cord injury in rats. Using rat model of spinal cord contusion injury at TVIII, we compared the effectiveness of immediate single transplantation of human mononuclear umbilical cord blood cells transfected with pBud-VEGF-FGF2 plasmid and immediate direct injection of t...

Pompe, M.; Veber, M., 2001: Prediction of rate constants for the reaction of O3 with different organic compounds. Volatile organic compounds (VOCs) of anthropogenic and biogenic origin play an important role in different photochemical processes in the atmosphere. In order to evaluate the lifetime of individual VOCs in the atmosphere and their impact on the ph...