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Ethanol ingestion increases activation of matrix metalloproteinases in rat lungs during acute endotoxemia

, : Ethanol ingestion increases activation of matrix metalloproteinases in rat lungs during acute endotoxemia. American Journal of Respiratory and Critical Care Medicine 160(4): 1354-1360

Previously we reported that alcohol abuse increases the incidence of the acute respiratory distress syndrome (ARDS) in septic patients, and that chronic ethanol ingestion in rats depletes alveolar epithelial glutathione and increases endotoxin-mediated lung edema. In this study we examined a potential mechanism by which ethanol-induced glutathione depletion could predispose to acute lung injury. We hypothesized that glutathione depletion activates matrix metalloproteinases (MMPs), thereby increasing degradation of the alveolar extracellular matrix (ECM) during sepsis. Ethanol-fed rats (20% vol/vol in water for 6 wk) were given endotoxin (2 mg/kg, intraperitoneally) followed 2 h later by lung isolation and ex vivo perfusion with n-formyl-methionyl-leucyl-phenylalanine (fMLP) (10(-)(7) M). Ethanol ingestion increased (p < 0.05) MMP-9 and MMP-2 activity, as determined by zymography, in the lung tissue and lavage fluid compared with control-fed rats, and increased (p < 0.05) levels of the 7S fragment of type IV collagen in the lung lavage fluid. Ethanol ingestion increased activation, but not production, of the MMP-9 and MMP-2 zymogens. Finally, although concomitant ingestion of N-acetylcysteine had no effect (p > 0.05) on MMP production, it increased (p > 0.05) lung glutathione levels, blocked (p < 0.05) MMP-9 and MMP-2 activation, and decreased (p < 0.05) levels of the 7S fragment of type IV collagen. We conclude that chronic ethanol ingestion, via glutathione depletion, activates MMPs during sepsis, thereby increasing degradation of the alveolar epithelial ECM. Lois M, Brown LAS, Moss IM, Roman J, Guidot DM. Ethanol ingestion increases activation of matrix metalloproteinases in rat lungs during acute endotoxemia.


PMID: 10508828

DOI: 10.1164/ajrccm.160.4.9811060

Other references

Ginsburg, I.; Borinski, R.; Pabst, M., 1985: NADPH and "cocktails" containing polyarginine reactivate superoxide generation in leukocytes lysed by membrane-damaging agents. Human blood leukocytes generated large amounts of superoxide (O2-) following stimulation by certain "cocktails" of soluble agents consisting of poly-L-arginine (PARG), phytohemagglutinin, the chemotactic peptide formyl-methionyl-leucyl-p...

Lim, T.L.W.; Tan, K.H.; Tee, C.S.; Yeo, G.S.H., 2005: Investigating stillbirths using a simplified obstetric events-based protocol. Introduction: A stillbirth remains a distressing enigma to parents and clinicians alike as the cause often remains elusive. Few papers describe a protocol for the investigation of stillbirths. We evaluate the first obstetric events-based protocol...

Fredin, R.A., 1964: Ocean mortality and maturity schedules of Karluk river sockeye salmon and some comparisons of marine growth and mortality rates. Four population models representing different temporal distributions of natural mortality during the ocean life of Karluk sockeye salmon (Onchorhynchus nerka) are developed and discussed. The first model simulates a constant monthly instantaneous...

Burin, P.; Mayer, G., 1962: Role of variations in sex steroid and corticosteroid levels in the appearance of the decidual reaction in the female rat. Comptes Rendus des Seances de la Societe de Biologie et de Ses Filiales 156: 1370-1372

Midgley, A.R.; Weiser, V.L., 1937: Effect of superphosphates in conserving nitrogen in cow manure. Grades of super. containing much gypsum are superior to those containing little. The effectiveness of the super. is enhanced by thorough mixture with the manure.

Kapitanovic, S.; Radosevic, S.; Slade, N.; Kapitanovic, M.; Andelinovic, S.; Ferencic, Z.; Tavassoli, M.; Spaventi, S.; Pavelic, K.; Spaventi, R., 2000: Expression of erbB-3 protein in colorectal adenocarcinoma: Correlation with poor survival. Background/aims: The family of erbB receptors includes four transmembrane glycoproteins with tyrosine kinase activity. These receptors are widely expressed in normal tissues, but they also have been implicated in the development of several human a...

Izawa Kiyoji; Tachibana Eizaburo; Inoue Yutaka, 1998: Evaluation of base-isolated effect in building structure for earthquake excitations; Part 1, Behavior of the base-isolated building for the Hyogo-Ken Nanbu earthquake. The seismic response of a base-isolated building structure is evaluated through the investigations of the observed records of the Hyogo-ken Nambu Earthquake. The 3-story base-isolated RC building and the ordinary building of 3-story steel frame st...

Kraevskaya L.N., 1936: Nekotoryye dannyye o poleznykh iskopayemykh Kurayskogo khrebta. Describes mineral resources, principally copper, of the Kurai range in the Altai mountains, Siberia, Russia.

Hay Roe, K.K., 1946: The helicopter in woodlands operations. A paper read by a director of the Bell Aircraft Corporation. It is claimed that the Bell Model 47 2-passenger helicopter, now in production, can make a gentle landing in a woodland clearing just large enough to give allround clearance to the rotor...

Frick O.L., 1969: Mediators of atopic and anaphylactic reactions rev human. Pediatric Clinics of North America 16(1): 95-107