+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Ethanol-mediated CYP1A1/2 induction in rat skeletal muscle tissue

, : Ethanol-mediated CYP1A1/2 induction in rat skeletal muscle tissue. Experimental and Molecular Pathology 69(3): 223-232

The causes of non-trauma-mediated rhabdomyolysis are not well understood. It has been speculated that ethanol-associated rhabdomyolysis may be attributed to ethanol induction of skeletal muscle cytochrome P450(s), causing drugs such as acetaminophen or cocaine to be metabolized to myotoxic compounds. To examine this possibility, the hypothesis that feeding ethanol induces cytochrome P450 in skeletal muscle was tested. To this end, rats were fed an ethanol-containing diet and skeletal muscle tissue was assessed for induction of CYP2E1 and CYP1A1/2 by immunohistochemical procedures; liver was examined as a positive control tissue. Enzymatic assays and Western blot analyses were also performed on these tissues. In one feeding system, ethanol-containing diets induced CYP1A1/2 in soleus, plantaris, and diaphragm muscles, with immunohistochemical staining predominantly localized to capillaries surrounding myofibers. Antibodies to CYP2E1 did not react with skeletal muscle tissue from animals receiving a control or ethanol-containing diet. However, neither skeletal muscle CYP1A1/2 nor CYP2E1 was induced when ethanol diets were administered by a different feeding system. Ethanol consumption can induce some cytochrome P450 isoforms in skeletal muscle tissue; however, the mechanism of CYP induction is apparently complex and appears to involve factors in addition to ethanol, per se.


PMID: 11115363

DOI: 10.1006/exmp.2000.2328

Other references

Singh, M.; Venkatasubramanian, R.; Balasubramanian, T.K.; Athalye, V.V., 1994: Setting up of an isotope analyser for quantitative determination of percentage abundance of 15 N in nitrogen gas. Analytical Letters 27(12): 2369-2376

Greenway A.M.; Rankine Jones A.I., 1992: Elemental concentrations in coastal sediments from hellshire jamaica. Twenty-four elements in the coarse and fine fractions (< 63 microns) of 37 coastal sediments have been determined by instrumental neutron activation analysis. The study was conducted in 50 km2 of coastal area stretching south south-west from th...

Ishikawa, D.; Hayashi, Y.; Takizawa, H., 2008: Preparation of platinum nanoparticles in heterogeneous solid-liquid system by ultrasound and microwave irradiation. Pt nanoparticles and Pt/spherical carbon (SC) were prepared in a heterogeneous (PtO2/SC, water/ethanol) solid-liquid system without dispersant using ultrasound and microwave irradiation. The Pt nanoparticles and Pt/SC were characterized using X-ra...

O.G.ady, J.; Sedano-Balbás, S.; Maher, M.; Smith, T.; Barry, T., 2007: Rapid real-time PCR detection of Listeria monocytogenes in enriched food samples based on the ssrA gene, a novel diagnostic target. A real-time PCR assay was designed to detect a 162-bp fragment of the ssrA gene in Listeria monocytogenes. The specificity of the assay for L. monocytogenes was confirmed against a panel of 6 Listeria species and 26 other bacterial species. A dete...

Humplik, B.I.; Sandrock, D.; Aurisch, R.; Richter, W-St.; Ewert, R.; Munz, D.L., 2005: Scintigraphic results in patients with lung transplants: a prospective comparative study. We addressed the feasibility of scintigraphy in the postoperative monitoring of lung transplants. 37 patients (22 women, 15 men, 37 +/- 15 years) in good clinical condition were examined after lung transplantation. Scintigraphic procedures for ass...

Ramos-Vara, J.A.; Webster, J.D., 2015: Special focus on investigative techniques. Veterinary Pathology 51(1): 5-6

Vose, J.M., 2008: Current status of rituximab maintenance therapy. Clinical Advances in Hematology & Oncology 5(12): 968-9,1006

V.P.Shcherbakov, 1982: On the theory of low-temperature oxidation of titanomagnetite grains. It is shown that the space-time distribution of vacancies in the process of formation of cation-deficient spinels is determined mainly by the boundary kinetics. At low rates of vacancy appearance in the surface layer (e.g., owing to oxygen deficit...

Baer; Drees; Godbole; Gunion; Tata, 1991: Phenomenology of light top squarks at the Fermilab Tevatron. Physical Review. D, Particles and Fields 44(3): 725-740

Gabe, Eduardo, D., 2005: Tiempo operatorio optimo en las valvulopatias. Cuando es tarde para la intervencion?. In this study we attemped to determine prospectively guidelines for the optimal management of patient with valvular heart disease.At present; most of valvular heart diseases are susceptible for surgical management It is suitable to perform the sur...