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Exhaled 8-isoprostane as an in vivo biomarker of lung oxidative stress in patients with COPD and healthy smokers

, : Exhaled 8-isoprostane as an in vivo biomarker of lung oxidative stress in patients with COPD and healthy smokers. American Journal of Respiratory and Critical Care Medicine 162(3 Pt 1): 1175-1177

Most of the studies linking chronic obstructive pulmonary disease (COPD) with oxidative stress are in vitro, using invasive techniques, or measuring systemic oxidative stress. The aim of this study was to quantify oxidative stress in the lungs in patients with COPD and in healthy smokers, as reflected by 8-isoprostane concentrations in breath condensate. This is a noninvasive method to collect airway secretions. 8-Isoprostane is a prostaglandin-F(2alpha) isomer that is formed in vivo by free radical-catalyzed peroxidation of arachidonic acid. We also studied the acute effect of smoking on exhaled 8-isoprostane in healthy smokers. Exhaled 8-isoprostane was measured by a specific enzyme immunoassay in 10 healthy nonsmokers and 12 smokers, 25 COPD ex-smokers, and 15 COPD current smokers. 8-Isoprostane concentrations were similar in COPD ex-smokers (40 +/- 3.1 pg/ml) and current smokers (45 +/- 3.6 pg/ ml) and were increased about 1.8-fold compared with healthy smokers (24 +/- 2.6 pg/ml, p < 0.001), who had 2.2-fold higher 8-isoprostane than healthy nonsmokers (10.8 +/- 0.8 pg/ml, p < 0.05). Smoking caused an acute increase in exhaled 8-isoprostane by about 50%. Our study shows that free radical production is increased in patients with COPD and that smoking causes an acute increase in oxidative stress.


PMID: 10988150

DOI: 10.1164/ajrccm.162.3.2001063

Other references

Galloway, D.A., 1993: Coping with a mentally and physically impaired infant: a self-analysis. Rehabilitation Nursing 18(1): 34-36

Deparis, M.; Auzepy, P.; Ferrane, J., 1964: Defense And Illustration Of Acute Pulmonary Congestion: Apropos Of 69 Cases. La Revue du Praticien 14: 1235-1259

Dziadek, M.; Richter, H.; Schachner, M.; Timpl, R., 1983: Monoclonal antibodies used as probes for the structural organization of the central region of fibronectin. Two monoclonal mouse antibodies against human plasma fibronectin were compared in their reactivity for proteolytic fragments of the antigen by enzyme immunoassay and immunoblotting. These antibodies were shown to react with two different structure...

Arboleda,C.; Mejia,A.I.; Franco-Molano,A.E.; Jimenez,G.A.; Penninckx,M.J., 2008: Autochthonous white rot fungi from the tropical forest of Colombia for dye decolourisation and ligninolytic enzymes production. Nineteen different strains of white rot fungi, originating from the tropical forest in Colombia were screened for their ability to decolourise Azure B and Coomassie Blue included in solid media. <i>Collybia plectophyla</i>, <i>Pl...

Cheong, J.Kong.; Gunaratnam, L.; Hsu, S.I-Hong., 2008: CRM1-mediated nuclear export is required for 26 S proteasome-dependent degradation of the TRIP-Br2 proto-oncoprotein. Overexpression of the proto-oncogene TRIP-Br2 (SERTAD2) has been shown to induce E2F activity and promote tumorigenesis, whereas ablation of TRIP-Br2 arrests cell proliferation. Timely degradation of many cell cycle regulators is fundamental to t...

Wu, Z.; Wei, G.; Lian, G.; Yu, B., 2010: Synthesis of mangiferin, isomangiferin, and homomangiferin. Mangiferin, isomangiferin, and homomangiferin, the xanthone C-glycosides with a wide spectrum of pharmacological effects, were synthesized concisely, featuring a C-glycosylation of a xanthene derivative with perbenzylglucopyranosyl N-phenyltrifluo...

Hobbs William Herbert, 1901: The river system of Connecticut. Journal of Geology Pages 469-485

Johnson P., 1966: Tuna fishing. Great Britain Ministry of Agriculture Fisheries & Food Directorate of Fisheries Research Laboratory Leaflet 14: 1-24

Liu, M.; Ruan, M.; Chen, L., 2015: Update on the molecular diagnosis and targeted therapy of thyroid cancer. Thyroid cancer (TC) is the most common endocrine malignancy with steadily increasing incidence over the past few decades. Although standard strategies for the management of TC offer optimal outcomes in TC patients with favorable histological types...

Monnier, J.F.; Raynaud, E.; Brun, J.F.; Orsetti, A., 1997: Influence of meal consumption and exercise on the assessment of body composition by bioelectrical impedance. The influence of both breakfast intake and exercise on the assessment of body composition by a multifrequency (1 to 100 kHz) device for bioelectrical impedance analysis was investigated in 11 professional football players. While the breakfast only...