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Expression of CD44 isoforms in normal salivary gland tissue: An immunohistochemical and ultrastructural study


, : Expression of CD44 isoforms in normal salivary gland tissue: An immunohistochemical and ultrastructural study. Histochemistry & Cell Biology 114(6): 483-488, December

We studied the expression of CD44 isoforms immunoreactivity in normal human salivary gland tissue, aiming at its full characterisation in normal epithelial and myoepithelial cell types. Optical immunohistochemistry techniques using monoclonal antibodies anti-CD44v3, CD44v4/5 and, for CD44v6, together with immunoelectron microscopy, were performed in serous, seromucinous and mucinous glands. Normal human breast and a case of lactating breast adenoma were used for comparative purposes and as controls. CD44v3 was positive in acinar and myoepithelial cells and was absent in mucin-producing cells from the different gland types. CD44v4/5 was consistently negative in all types of salivary tissue. CD44v6 was constantly positive in serous acinar cells, focally positive in basal cells of ducts, and myoepithelial cells consistently expressed it. At the ultrastructural level, CD44v6 was localised to the inter-digitating processes of acinar cells, whenever they were not covered by basal lamina and to the cell membrane facing myoepithelial cells. In myoepithelial cells, immunolabelling was found at the membranes facing the acinar cells and in caveolae present at this interface. No labelling was found at cell membranes of both acinar and myoepithelial cells in contact with basal lamina or at the luminal aspect of the former. The finding of CD44v3 and v6 in myoepithelium of normal salivary glands may argue in favour of the role of these molecules in the regulation of growth and renewal of normal tissues and, potentially, on the morphogenesis of salivary gland neoplasms.

US$19.90

DOI: 10.1007/s004180000220


Other references

Wong, T.T.Y.; Madsen, H.F., 1967: Laboratory and field studies on the seasonal forms of pear psylla in northern California. The pear psylla, Psylla pyricola Foerster, has 2 forms. The summer form is generally of light color, and is observed in the fields from the beginning of spring until autumn; the winter form is darker and larger, and is present from autumn to sprin...

Darialashvili, A.A., 1962: Some data on the composition of the sediment in the colored urine precipitation test. Studies of O2, N2 and Ag content in the silver precipitate before and after treatment with charcoal showed that this precipitate contains both nitrogenous and non-nitrogenous organic compounds, regardless of the color or specific gravity of the ur...

Takagi, H.; Furuya, N.; Kojima, N., 2007: Preferential production of IL-12 by peritoneal macrophages activated by liposomes prepared from neoglycolipids containing oligomannose residues. The present study demonstrates that liposomes coated with synthesized neoglycolipids constructed from mannotriose and dipalmitoylphosphatidylethanolamine (Man3-DPPE) activate peritoneal macrophages (PEMs) to up-regulate expression of co-stimulator...

Yilmaz, H.Baki.; Celebi, S.; Sahin-Yilmaz, A.; Oysu, C., 2014: The role of mometasone furoate nasal spray in the treatment of adenoidal hypertrophy in the adolescents: a prospective, randomized, cross-over study. Aim of this work is to find out whether the symptoms attributable to adenoid hypertrophy in adolescents may be treated with intranasal mometasone furoate (MF) application. To learn if adenoid hypertrophy in adolescents may decrease in size with in...

Van Der Weyden, M.B., 2003: Researchers as guinea pigs. Medical Journal of Australia 178(2): 52-53

Nortjé, C.J., 2014: Maxillo-facial radiology case 118. Oculodentodigital dysplasia. Sadj 69(2): 76-76

Otero, G.; Berkovich, A.; Clerch, L.; Massaro, D.; Notario, V., 1997: Radiation-initiated, immortal Syrian hamster embryo fibroblasts release elevated levels of H2O2 and show divergent MnSOD and catalase activities. To characterize molecular events associated with the neoplastic conversion of primary cells by ionizing radiation, we studied the activities and mRNA expression of manganese superoxide dismutase (MnSOD) and catalase (CAT) in Syrian hamster embryo...

Djaldetti M.; Gilgal R.; Creter D.; Zahavi I., 1988: The in vitro effect of pirprofen on the surface ultrastructure and function of human platelets. The in vitro effect of pirprofen (Rengasil), an antiinflammatory agent, on the surface ultrastructure and function of human platelets was examined and compared with that of acetylsalicylic acid (Aspirin, ASA), and diclofenac sodium (Voltaren, DS)....

Bailey, C.A.; Railkar, A.; Tarantino, R., 1996: Along the development superhighway At the interface between discovery and development of small molecule therapeutics. Abstracts of Papers American Chemical Society 211(1-2): BTEC 12

Spruill, W.A.; White, M.G.; Steiner, A.L.; Tres, L.L.; Kierszenbaum, A.L., 1981: Temporal sequence of cell shape changes in cultured rat sertoli cells after experimental elevation of intracellular cAMP. Experimental Cell Research 131(1): 131-148