geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Expression of gamma-glutamyl transpeptidase in mouse perivascular astrocytes and in a protoplasmic-like astroglial cell clone


, : Expression of gamma-glutamyl transpeptidase in mouse perivascular astrocytes and in a protoplasmic-like astroglial cell clone. Brain Research 852(1): 191-197

gamma-Glutamyl transpeptidase (GGT) is known to be present in the central nervous system (CNS) but its cellular localization is still subject to controversy. In this report, we have investigated, with a specific antiserum, the immunolabelling pattern of GGT in the adult mouse CNS at the light and electron microscopic (EM) levels. At the optical level, GGT immunoreactivity ensheathes the majority of vessels in the grey matter. Immunoelectron microscopy shows that labelling is essentially due to the presence of GGT in the astrocytic endfeet which surround vessels. In addition, some pericytes and periendothelial cells are also clearly labelled. We then investigated GGT activity in astroglial cell clones which may represent the in vitro counterpart of the main astroglial cell types. The striking result is that a protoplasmic-like astroglial cell clone shows a noticeable GGT activity, while, in contrast, no activity was detected in the fibrous and the Golgi-Bergmann-like astroglial clones. Taken together, these data indicate that, in the mouse CNS, GGT is essentially present in protoplasmic astrocytes.

US$29.90

PMID: 10661512


Other references

Lebrun J P.; Gaston A., 1986: Third supplement to the catalogue of vascular plants of southern chad. 41 species were newly recorded from southern Chad, the flora of which now amounts to 1547 species.

Taglialatela, G.; Navarra, D.; Olivi, A.; Ramacci, M.T.; Werrbach-Perez, K.; Perez-Polo, J.R.; Angelucci, L., 1995: Neurite outgrowth in PC12 cells stimulated by acetyl-L-carnitine arginine amide. Senescence of the central nervous system is characterized by a progressive loss of neurons that can result in physiological and behavioral impairments. Reduction in the levels of central neurotrophic factors or of neurotrophin receptors may be one...

Ivandini, T.A.; Honda, K.; Rao, T.N.; Fujishima, A.; Einaga, Y., 2007: Simultaneous detection of purine and pyrimidine at highly boron-doped diamond electrodes by using liquid chromatography. Highly boron-doped diamond (BDD) electrode, have been examined for simultaneous detection of purine and pyrimidine bases in mild acidic media by using HPLC with amperometric detection. Cyclic voltammetry at as-deposited (AD) and anodically oxidiz...

Zucker, L.M., 1947: Evaluation of slopes and intercepts of straight lines. Many scientifically useful quantities are estimated not by direct measurement but must be calculated from the slope or intercept of a straight line fitted to a set of points. Assuming that errors are essentially random and have a normal Gaussian p...

McNeill, C., 1996: Vive la différence. Journal of Orofacial Pain 10(3): 197-198

Pattison, T.L.; Lindsay, D.; Von Holy, A., 2003: In vitro growth response of bread-spoilage Bacillus strains to selected natural antimicrobials. This study assessed the in vitro responses of Bacillus (B.) strains isolated from ropey bread to natural antimicrobials under optimum growth conditions. The responses of six Bacillus strains [B. subtilis (2), B. licheniformis (2) and B. pumilus (2...

Glassberg, J., 1997: Method for forensic analysis. Official Gazette of the United States Patent & Trademark Office Patents 1194(2): 1186, Jan 14

Kitajima, E.W.; Martins, M.C.; Barros, T.S.L.; Bedendo, I.P.; Frare, P.M., 1999: Occurrence of chayote witches' broom at Amparo county, SP, Brazil. This note reports the occurrence of chayote (Sechium edulis) witches' broom at Amparo county, State of Sao Paulo, Brazil. Based on electron microscopy and PCR assay, the causal agent was identified as a phytoplasma. RFLP analysis of the PCR-a...

Wells, C.R.; Goldschmidt, P.J.; Coleman, L.D.; Coombs, V.J.; Gerstenblith, G.; Brinker, J.A.; Resar, J.R., 1997: Platelet aggregation and fibrinogen binding in patients treated with integrelin and abciximab during coronary angioplasty. Journal of the American College of Cardiology 29(2 SUPPL A): 241A

Anonymous, 2010: Proceedings from the Paris Dermatology Meeting, 10 December 2008. Annales de Dermatologie et de Venereologie 136 Suppl 2: S21-S32