+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Expression of the rfa, LPS biosynthesis promoter in Salmonella typhimurium during invasion of intestinal epithelial cells

, : Expression of the rfa, LPS biosynthesis promoter in Salmonella typhimurium during invasion of intestinal epithelial cells. Current Microbiology 41(3): 172-176, September

Salmonella regulates transcription of many of its genes in response to environmental conditions encountered inside or outside the eukaryotic cells it infects. In this paper, we examined Salmonella typhimurium gene expression within epithelial cells, by using bacterial luciferase as a reporter. We focused on gene expression controlled by Salmonella rfa promoter, using lac promoter as a control. We observed down regulation for both promoters during the initial 2 h of invasion. The decreased levels of luciferase activity appeared to be due to metabolic changes, since we observed similar results with tissue culture medium alone. Gene expression stabilized to a new steady state for the Salmonella rfa promoter, while a lac promoter activity steadily decreased. Bacterial luciferase activity was a good indicator of intracellular numbers and allowed us to detect as few as 1000 bacterial cells/infected monolayer. Both promoters were not dependent on host protein synthesis for expression.


PMID: 10915202

DOI: 10.1007/s002840010113

Other references

Kim, J.Heon.; Lee, B.; Chung, B.I., 2017: Re: Philipp Mandel, Felix Preisser, Markus Graefen, et al. High Chance of Late Recovery of Urinary and Erectile Function Beyond 12 Months After Radical Prostatectomy. Eur Urol 2017;71:848-50. European Urology 72(6): E175-E175

Kotze J.P.; Steinberg D., 1971: Effects of free fatty acid infusion on glucose metabolism in diabetic dogs. Circulation Supplement 44(4): II-19

Treus, M., 1970: Chemical composition and biological properties of eland milk. Acidity in eland milk increased much more slowly than in cow's milk. The milk fat was 9 to 14%, fat constant 25.2%, the iodine number 40.5, total protein 8 to 9%, soluble proteins 1 to 2%, ratio of fat to protein 1.4, casein content 7 to 8%,...

Anderson, P.A.M., 2013: Giving feedback on clinical skills: are we starving our young?. Feedback to clinical learners about their performance is crucial to their development into competent clinicians. Most feedback is provided by clinicians who have little or no formal training for this aspect of their teaching role. Narrative review...

Dobrovol'skii G.V.; Orlov D.S.; Grishina L.A., 1983: Principles and goals of soil monitoring. Problems of soil protection are considered as part of environment protection in global and national aspects. The necessity of soil monitoring is substantiated for our country, and principles of its development and immediate goals are formulated.

Neagu T., 1992: White chalk foraminiferal fauna from southern Dobrogea (Romania); III, Calcareous benthic foraminifera. Revista Espanola de Micropaleontologia 24(1): 509-565

Tarakanov, P., 1991: Genetic variability and evaluation of heritability of cocksfoot hybrids according to dry matter yield. In tests of 24 F1 hybrids during 1987-88, genetic variation for yield was low in the first year and was associated with a high dry matter yield, but increased in the second year. Heritability estimates showed the same pattern as genetic variation....

Uzé, M.; Potrykus, I.; Sautter, C., 1999: Single-stranded DNA in the genetic transformation of wheat (Triticum aestivum L.): transformation frequency and integration pattern. Two non-linked marker genes (gus and bar) were co-introduced by microprojectile bombardment into wheat cells. Four different DNA structures were compared with respect to ability to integrate into the wheat genome: circular or linear (1) DNA as a s...

Ardoin P., 1973: Tenebrionidae coleoptera from sardinia. Annales de la Societe Entomologique de France 9(2): 257-307

Schmidt, L., 2010: Physiotherapy in rheumatic diseases; the choice of suitable methods at various stages. British Journal of Physical Medicine 9: 104-110