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Extraction and purification of soil microbial total DNA

, : Extraction and purification of soil microbial total DNA. Weishengwu Xuebao 43(2): 276-282, April

A method of extracting soil total DNA was developed, and it can extract DNA from G+ bacteria. The soil total DNA was extracted from 9 different soils, the DNA yeild was from 3.30mug to 13.41mug. The crude soil DNA was purified by dialysis bag, the recovery rate is 65.34%. The extracted rate in the 9 soils was 60.51%apprx93.45%. The traget gene could be amplified from the soil which was added 362 target cell/g dry soil.


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