geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Formation of DNA adducts by microsomal and peroxidase activation of p-cresol: Role of quinone methide in DNA adduct formation


, : Formation of DNA adducts by microsomal and peroxidase activation of p-cresol: Role of quinone methide in DNA adduct formation. Chemico-Biological Interactions 138(3): 217-229, December 21

We have investigated the activation of p-cresol to form DNA adducts using horseradish peroxidase, rat liver microsomes and MnO(2). In vitro activation of p-cresol with horseradish peroxidase produced six DNA adducts with a relative adduct level of 8.03+/-0.43 x 10(-7). The formation of DNA adducts by oxidation of p-cresol with horseradish peroxidase was inhibited 65 and 95% by the addition of either 250 or 500 microM ascorbic acid to the incubation. Activation of p-cresol with phenobarbital-induced rat liver microsomes with NADPH as the cofactor; resulted in the formation of a single DNA adduct with a relative adduct level of 0.28+/-0.08 x 10(-7). Similar incubations of p-cresol with microsomes and cumene hydroperoxide yielded three DNA adducts with a relative adduct level of 0.35+/-0.03 x 10(-7). p-Cresol was oxidized with MnO(2) to a quinone methide. Reaction of p-cresol (QM) with DNA produced five major adducts and a relative adduct level of 20.38+/-1.16 x 10(-7). DNA adducts 1,2 and 3 formed by activation of p-cresol with either horseradish peroxidase or microsomes, are the same as that produced by p-cresol (QM). This observation suggests that p-cresol is activated to a quinone methide intermediate by these activation systems. Incubation of deoxyguanosine-3'-phosphate with p-cresol (QM) resulted in a adduct pattern similar to that observed with DNA; suggesting that guanine is the principal site for modification. Taken together these results demonstrate that oxidation of p-cresol to the quinone methide intermediate results in the formation of DNA adducts. We propose that the DNA adducts formed by p-cresol may be used as molecular biomarkers of occupational exposure to toluene.

US$19.90

PMID: 11714480

DOI: 10.1016/s0009-2797(01)00274-5


Other references

Rao, G.K.; Balasubramanian, K.A.; Sathyanarayana, 1989: Contribution of some prime plant characters in relation to sorghum charcoal rot disease. Reduced leaf area, fewer number of leaves and taller plant stature were all clearly associated with charcoal rot (Macrophomina phaseolina) resistance when combined mean values of 2 resistant and 2 susceptible genotypes were compared.

Aldrees, T.; Alanazi, A.; Fatani, H.A.; Samman, A.; Aldhahri, S.F., 2016: Adenoid cystic carcinoma of the upper airway mimicking a thyroid tumor: A case report. NlmCategory="UNASSIGNED">Adenoid cystic carcinoma (ACC) is a rare malignant tumor, reportedly representing <1% of all head and neck cancers. There have been few reported cases of ACC of the upper airway presenting as a midline mass...

Candeias, J.A.; Himelfarb, L., 1966: Detection of complement fixing antibodies against respiratory syncytial virus in inhabitants of the city of São Paulo. Arquivos Da Faculdade de Higiene E Saude Publica Da Universidade de Sao Paulo 20(2): 207-213

Skopouli, E.N., 2004: Do clinical studies add valuable information to a syndrome described 70 years ago?. Autoimmunity Reviews 3 Suppl 1: S60-S61

Clowes, M.S.J., 1980: Ripening activity of the glyphosate salts Mon 8000 and Roundup. Mon 8000 (600 g a.e./kg) and Roundup (359 g a.e./litre) produced similar ripening responses (mainly on cv. N:Co.376) when compared at 1 kg and 1.5 litres/ha or 0.67 kg and 1 litre/ha, respectively. Improvement in cane quality persisted for up to 2...

Sanchez-Castro; Bedell, 1991: Two-component Fermi liquids and the induced-interaction model. Physical Review. B, Condensed Matter 43(16): 12874-12887

Crissman, J.D.; Henson, S.L., 1978: Malignant fibrous histiocytoma of the maxillary sinus. Malignant fibrous histiocytomas are an unusual neoplasm in the head and neck, and only six cases involving the maxillary sinus have been reported. The combination of an anaplastic storiform "fibroblastic" stroma with xanthomatous tumor g...

Tani, H.; Fukuma, A.; Fukushi, S.; Taniguchi, S.; Yoshikawa, T.; Iwata-Yoshikawa, N.; Sato, Y.; Suzuki, T.; Nagata, N.; Hasegawa, H.; Kawai, Y.; Uda, A.; Morikawa, S.; Shimojima, M.; Watanabe, H.; Saijo, M., 2016: Efficacy of T-705 (Favipiravir) in the Treatment of Infections with Lethal Severe Fever with Thrombocytopenia Syndrome Virus. Severe fever with thrombocytopenia syndrome virus (SFTSV) is the causative agent of SFTS, an emerging hemorrhagic fever. This disease has a high case fatality rate and is endemic to China, South Korea, and Japan. Because there are currently no eff...

Kerkhof, P.L.M.; Yasha Kresh, J.; Li, J.K-J.; Heyndrickx, G.R., 2013: Left ventricular volume regulation in heart failure with preserved ejection fraction. Ejection Fraction (EF) has attained the recognition as indicator of global ventricular performance. Remarkably, precise historical origins promoting the apparent importance of EF are scant. During early utilization EF has been declared a gold stan...

Gono, T.; Yazaki, M.; Agematsu, K.; Matsuda, M.; Yasui, K.; Yamaura, M.; Hidaka, F.; Mizukami, T.; Nunoi, H.; Kubota, T.; Ikeda, S-Ichi., 2008: Adult onset X-linked chronic granulomatous disease in a woman patient caused by a de novo mutation in paternal-origin CYBB gene and skewed inactivation of normal maternal X chromosome. We report a 28-year-old woman patient suffering from refractory subcutaneous abscess. Stimuli-induced microbicidal reactive oxygen metabolites formation test of the patient's neutrophils revealed that only 9.6% of the neutrophils produced H2O...