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Functional protective role for mucin glycosylated repetitive domains

, : Functional protective role for mucin glycosylated repetitive domains. European Journal of Biochemistry 266(1): 105-111, Nov

Mucins carry out a number of protective roles, some of which are more easily studied than others. One mucin function is believed to be the protection of the mucosal epithelium against acidic and proteolytic damage in the stomach and intestines. In the present work, a portion of stomach mucin tandem repeat sequence (Muc6) was joined to the catalytic domain of a reporter enzyme (human milk cholesterol esterase (CE)) to determine whether the former can protect the latter protein from damage. This Muc6 domain replaced a unique series of glycosylated C-terminal repeats normally present in CE. The chimeric protein (CE/Muc6) was expressed in two different cell lines and its properties compared to recombinant full-length CE and a truncated version of CE which contained only the catalytic domain (CE/trunc). Results showed that both CE and CE/Muc6 were resistant to denaturation by acid and to proteolysis by pepsin at low pH values or by pancreatic proteases compared to CE/trunc. Thus, a stomach Muc6 domain is sufficient to confer stability on the CE catalytic domain, demonstrating a protective effect by a glycosylated mucin sequence.


PMID: 10542055

DOI: 10.1046/j.1432-1327.1999.00824.x

Other references

Zamboni G.; Tagliaro F.; Mantovanelli F.; Tato L., 1990: Monomeric calcitonin in infants and children. It has been recently that the determination of extracted calcitonin provides major specificity than immunoreactive calcitonin for the evaluation of calcitonin secretion in physiological and pathological conditions. In this study we evaluated blood...

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Ramsay, K.; Bergmann, M.; Veale, L.; Richardson, C.; Kaiser, M.; Vize, S.; Feist, S., 2001: Damage, autotomy and arm regeneration in starfish caught by towed demersal fishing gears. Arm damage and loss were examined in the starfish Asterias rubens that had been caught in a variety of towed commercial fishing gears deployed on different sea bed types. Between 7% and 38% of the starfish in each catch lost one or more arms, and...

Durie, P.R.; Bell, L.; Linton, W.; Corey, M.L.; Forstner, G.G., 1980: Effect of cimetidine and sodium bicarbonate on pancreatic replacement therapy in cystic fibrosis. Fifteen patients with cystic fibrosis and pancreatic insufficiency were studied during four randomised seven day treatment periods in which they received only pancreatic supplement (Pancrelipase, 27 capsules per day) or supplement plus cimetidine...

Mueller H.J., 1985: On the duration of the parapause in mocydia crocea homoptera auchenorrhyncha and its ecological significance. The monovoltine leafhopper Mocydia crocea winters with an endogenous ovarial dormancy, a photoperiodic conditioned parapause, for termination requiring short days until midwinter, afterwards governed only by thermic quiescence until spring. In fol...

Holstrom S.E., 1989: Feline dentistry ii. Journal of the American Veterinary Medical Association 194(12): 1812

Ryou, M.; Hasegawa, K.; Kowa, H.; Yagishita, S., 1996: Does promoting aging process exist in suffering from motor neuron disease?. Nakano, I [Editor], Hirano, A [Editor] International Congress Series; Amyotrophic lateral sclerosis: Progress and perspectives in basic research and clinical application 87-92

Anonymous, 1986: Eightieth birthday of academican of the Academy of Sciences of the Ukrainian SSR, S.M.Gershenzon. Piopolimery i kletka 2(2): 112

Skaug, M.J.; Lacasta, A.M.; Ramirez-Piscina, L.; Sancho, J.M.; Lindenberg, K.; Schwartz, D.K., 2014: Single-molecule diffusion in a periodic potential at a solid-liquid interface. We used single-molecule tracking experiments to observe the motion of small hydrophobic fluorescent molecules at the interface between water and a solid surface that exhibited periodic chemical patterns. The dynamics were characterized by non-ergo...

Bicknell, R.J.; Young, P.W.; Schofield, J.G.; Albano, J., 1977: Mechanism of action of somatostatin: growth-hormone release [45Ca]calcium ion efflux and cyclic nucleotide metabolism of bovine anterior-pituitary slices in the presence of prostaglandin E2 and 1-methyl-3-isobutylxanthine. Biochemical Society Transactions 5(1): 219-222